This study solidifies the aeolian sand by microbial induced carbonate precipitation (MICP) technique. The effects of cementation solution with different concentrations, particle size, and grouting batches are examined via the bender element, unconfined compressive test and scanning electron microscope (SEM). The bender element results show that the wave speed of loose aeolian sand is 200m/s; however, after solidify the aeolian sand, the speed of P-wave is about 450-600m/s and S-wave is about 350-500m/s. Additionally, the unconfined compressive strength (UCS) results indicate that when the concentration of cementation solution is 0.75mol/L, the UCS of bio-solidified sand sample is the highest. Then, compared with the aeolian sand with original grade, the particles ranging from 0.1-0.4mm have a better cementation effect. Moreover, the UCS of bio-solidified sand samples increases along with the grouting batch. From the SEM images, it can be seen that when the grouting batch reaches to five times, the particles are almost completely covered by CaCO 3 crystals compared with the three batches and four batches.