Effect of Sodium Sulphate on Tissue Cultures of Brassica napus cv. Westar and Brassica campestris L. cv. Tobin

Chandler, Stephen F.; Mandal, B. B.; Thorpe, Trevor A.

doi:10.1016/s0176-1617(86)80223-1

Cited by 16 publications

(3 citation statements)

References 24 publications

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“…6 and 7. Similar results were reported by Chandler et al (1986), Raldugina and Peskova (1988) and Mandal et al (1989) Data are transformed according to Steel & Torrie (1980). …”

Section: In Vitro Selection Of Salt Tolerate Callisupporting

confidence: 81%

Devolopment of Some Canola Genotypes to Salinity Tolerance Using Tissue Culture Technique

Abdrabou¹,

Fergani²,

Azzam³

et al. 2017

Egypt. J. Agron.

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, to study the variation among canola varieties in response to salt tolerance through tissue culture propagation. This work was aimed to establish a regeneration protocol for canola genotypes, production of salt-tolerance plants through selection of salttolerance callus via somaclonal variation and subsequent regeneration of plantlets from such callus. Four genotypes of canola were used for establishment a regeneration protocol, Bingo and Torpe were uesed as highly salt tolerant genotypes while, Conny and Siberian as susceptible genotypes. The results indicated that MS medium supplemented with 2 mg/l 2,4-D was the successive medium for callus formation and shoot regeneration was obtained from MS medium supplemented with 5 mg/l BA + 0.05 NAA, while, 1mg/l IBA was the better growth regulator for rooting.For in vitro selection of salt tolerant calli, Torpe (genotype overcomes the others in calli survival percentages under 8000, 12000 and 16000 ppm NaCl concentrations. The results of shoot frequency from tolerant calli cleared that Torpe genotype recorded the highest value of shoot percentage followed by Bingo genotype, whereas, Conny followed by Siberian genotypes were recorded the lowest values. At 16000 ppm NaCl concentration, only tolerant calli of Torpe genotype was able to initiat shoot under this concentration. For acclamatization after regeneration from the tolerant calli, only five salt tolerant plantlets developed from Torpe genotype and eight salt tolerant plantlets developed from Siberian genotype that succeeded to complete their life cycle and reached maturity stage and produced seeds. These regenerated genotypes were referred as T1, T2, T3, T4 and T5, the five regenerated plants developed from Torpe genotype that tolerate NaCl concentration up to 16000 ppm, as well as, S1, S2, S3, S4, S5, S6, S7 and S8, the eight regenerated plants developed from Siberian genotype that tolerate NaCl concentration up to 12000 ppm.

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“…6 and 7. Similar results were reported by Chandler et al (1986), Raldugina and Peskova (1988) and Mandal et al (1989) Data are transformed according to Steel & Torrie (1980). …”

Section: In Vitro Selection Of Salt Tolerate Callisupporting

confidence: 81%

Devolopment of Some Canola Genotypes to Salinity Tolerance Using Tissue Culture Technique

Abdrabou¹,

Fergani²,

Azzam³

et al. 2017

Egypt. J. Agron.

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show abstract

“…If sodium sulfate does act in this way, the results also indicate that induction was only possible in young callus, as shoots could not be induced in established control callus. Finally, our observations do not appear to be confined to tobacco, as indicated by a number of recent reports where water and salt stress have been shown to promote organogenesis in vitro [1,5,7,8]. However, the mechanism for this ~ phenomenon remains to be determined.…”

Section: Discussionmentioning

confidence: 50%

Some morphogenic effects of sodium sulfate on tobacco callus

Chandler

Ragolsky

Pua

et al. 1987

Plant Cell Tiss Organ Cult

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Callus cultures of Nicotiana tabacum L cv. Wisconsin 38 were initiated and grown on shoot-forming (SF) and callus proliferation (CP) medium with or without Na2SO 4. Two cultures were maintained on SF medium with 0, 0.75, 1 or 1.5% Na2SO 4 for 2.5 and 3.5 years. In the older culture only callus grown on salt formed shoots throughout the maintenance period, while in the younger culture the control responded best and Na 2 SO 4 was inhibitory. Callus from the older culture which had been grown on salt continued to form shoots in the absence of salt. Na 2 SO 4 caused adventitious shoot formation in three cultures on CP medium. These shoots were present for 7 subcultures after removal of Na2 SO4; but established, control callus, did not form shoots when transferred to Na 2 SOn. Callus initiated and maintained on NaC1 or mannitol showed a slight increase in shoot initiation. On NaC1, Na 2 SO4 or mannitol, the tissue osmotic potential became more negative and proline concentration increased.

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“…In vitro culture is a valid and rapid tool for studying the behavior of plants in response to salt stress, while other factors (nutrients, lighting, temperature) are held constant and controlled in an optimal manner. This approach has been successfully used for the evaluation of salt stress in brassica [8], tomato [9][10][11][12][13][14][15][16], mulberry [17,18], potato [19], rice [20][21][22], poplar [23,24], strawberry [25], eucalyptus [26][27][28], grapevine [29,30], citrus [31,32], and pistacia [33].…”

Section: Introductionmentioning

confidence: 99%

Alfalfa (Medicago sativa L.) clones tolerant to salt stress: in vitro selection

et al. 2013

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In order to quickly and efficiently evaluate the salt tolerance of alfalfa, salinity tests were conducted on Medicago sativa L. var. australis, var. icon, var. loi, and var. gea, under in vitro conditions. Pregerminated seeds of four varieties were subjected to five different NaCl concentrations (0, 50, 100, 150, 200 mM). The influence of saline stress was estimated on the basis of survival percentage, growth parameters, and electrolyte leakage. The seedlings surviving on the medium enriched with salt at the highest concentration were presumed to be tolerant and represented the mother plants for the production of in vitro clones. In the following step, the clones were evaluated in vitro to confirm the salt tolerance. The influence of mild salt stress (75 mM NaCl) on the growth parameters of selected clones was examined. At the end of this trial, the proline accumulation and sodium content in alfalfa shoots were also quantified. The results suggest an increased level of proline promotes salt tolerance. Medicago sativa L. var. icon is highly tolerant in comparison with the other varieties tested. In vitro selection of M. sativa L. varieties on salt-containing media allowed us to obtain clones with increased salinity tolerance.

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Effect of Sodium Sulphate on Tissue Cultures of Brassica napus cv. Westar and Brassica campestris L. cv. Tobin

Cited by 16 publications

References 24 publications

Devolopment of Some Canola Genotypes to Salinity Tolerance Using Tissue Culture Technique

Devolopment of Some Canola Genotypes to Salinity Tolerance Using Tissue Culture Technique

Some morphogenic effects of sodium sulfate on tobacco callus

Alfalfa (Medicago sativa L.) clones tolerant to salt stress: in vitro selection

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