Effect of sodium chloride on the thermodynamic, rheological, and microstructural properties of field pea protein isolate/chitosan complex coacervates

Zhang, Qing; Jeganathan, Brasathe; Dong, Hongmin; Chen, Lingyun; Vasanthan, Thava

doi:10.1016/j.foodchem.2020.128569

Cited by 22 publications

(15 citation statements)

References 54 publications

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“…When the value of pH was greater than 7.0, the turbidity increased rapidly, forming insoluble aggregates and causing light scattering. This is consistent with the previous results suggesting that turbidity values of CS solutions increase after pH exceeds 7.0 [ 38 ]. For the aqueous STI solution, the turbidity was low when the pH was less than 4.0 or greater than 6.5.…”

Section: Resultssupporting

confidence: 94%

Interactions between Soybean Trypsin Inhibitor and Chitosan in an Aqueous Solution

Zhang

Liu

et al. 2023

Polymers

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Supramolecular structures obtained from protein–polysaccharide association may be applied to encapsulate bioactive compounds or to improve the physical stability and texture properties of colloid–based products. In this study, the interaction of 0.1 wt% soybean trypsin inhibitor (STI) with different concentrations of chitosan (CS) in aqueous solutions was investigated under different pH by the analysis of state diagram, turbidity, zeta potential, spectroscopy, and microstructure; the protective effect of STI–CS complex coacervates on STI stability in simulated gastric juice was also discussed. The results suggested that interactions between STI and CS could form soluble/insoluble complexes mainly through hydrophobic interactions (pH 4.0) or electrostatic interactions (pH 6.0). The CD spectra showed that the secondary structure of STI did not change significantly when CS with the same charge was mixed with STI, and the secondary structure of STI was slightly changed when CS with the opposite charge was mixed with STI. Simulated gastric digestion experiments showed that the complex formed by non-covalent bonding had a protective effect on the active protein. This study provides information about the effect of different CS concentrations and pH values on the formation of complexes of CS and STI in an aqueous solution and provides theoretical references for the construction of supramolecular-structured carrier substances based on CS and STI.

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Section: Resultssupporting

confidence: 94%

Interactions between Soybean Trypsin Inhibitor and Chitosan in an Aqueous Solution

Zhang

Liu

et al. 2023

Polymers

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“…The complex formation through electrostatic interaction is strongly influenced by the ionic strength of the medium. , With a higher ionic strength, more dissociation of the complexes was observed, namely, the electrostatic shielding effect. When NaCl was added into the systems, i.e., CS/ssDNA complexes and CS-PHEMA/ssDNA complexes, and the concentrations were tuned from 50 to 500 mM, the decrease in the zeta potential from positive to negative was observed (Figure S7).…”

Section: Resultsmentioning

confidence: 99%

Brush-Structured Chitosan/PolyHEMA with Thymine and Its Synergistic Effect on the Specific Interaction with ssDNA and Cellular Uptake

2022

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Cationic polymers are known to attach on an anionic cell surface and favor gene transportation/transfection into the cells. However, when the positive charges accumulate, they tend to cause cell damage and delivery failure. Chitosan (CS) is a potential cationic bio-derived polymer whose chemical structures can be modified to fine-tune the charges as well as the add-on functions. The present work demonstrates (i) the decoration of a nucleic acid sequence-like brush structure on CS to allow the specific interaction with DNA and (ii) delivery into the cell. By simply applying mercaptoacetic acid as the chain transfer agent, the grafting of poly(hydroxyethyl methacrylate) (PHEMA) containing Thy (P(HEMA-Thy)) on CS is possible. The brush-like P(HEMA-Thy) leads Thy moieties to be in sequences. The Thy sequences perform as poly [T] for the specific interaction with ssDNA. The synergistic effect of CS and Thy sequences, i.e., electrostatic and base pairing interactions, results in an effective and efficient binding with ssDNA as well as significant delivery, especially in cellular uptake and cell viability. The use of CS in combination with Thy sequences in brush-like structures on CS is a model for other polysaccharides to be conjugated with the as-designed nucleic acid sequences for potential gene delivery.

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“…[ 45,47,[58][59][60][61][62][63][64][65][66][67][68][69][70][71][72] Entropy gain dominated complex coacervation (ΔH > 0, ΔS > 0)…”

Section: Underlying Mechanismmentioning

confidence: 99%

New insights into protein–polysaccharide complex coacervation: Dynamics, molecular parameters, and applications

Zheng,

Van der Meeren,

Sun

2023

Aggregate

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For more than a decade, the discovery of liquid–liquid phase separation within living organisms has prompted colloid scientists to understand the connection between coacervate functionality, phase behavior, and dynamics at a multidisciplinary level. Although the protein–polysaccharide was the first system in which the coacervation phenomenon was discovered and is widely used in food systems, the phase state and relaxation dynamics of protein–polysaccharide complex coacervates (PPCC) have rarely been discussed previously. Consequently, this review aims to unravel the relationship between PPCC dynamics, thermodynamics, molecular architecture, applications, and phase states in past studies. Looking ahead, solving the way molecular architecture spreads to macro‐functionality, that is, establishing the relationship between molecular architecture–dynamics–application, will catalyze novel advancements in PPCC research within the field of foods and biomaterials.

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Effect of sodium chloride on the thermodynamic, rheological, and microstructural properties of field pea protein isolate/chitosan complex coacervates

Cited by 22 publications

References 54 publications

Interactions between Soybean Trypsin Inhibitor and Chitosan in an Aqueous Solution

Interactions between Soybean Trypsin Inhibitor and Chitosan in an Aqueous Solution

Brush-Structured Chitosan/PolyHEMA with Thymine and Its Synergistic Effect on the Specific Interaction with ssDNA and Cellular Uptake

New insights into protein–polysaccharide complex coacervation: Dynamics, molecular parameters, and applications

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