Effect of sodium chloride-induced stress on growth, proline, glycine betaine accumulation, antioxidative defence and bacoside A content in in vitro regenerated shoots of Bacopa monnieri (L.) Pennell

Ahire, Mahendra L.; Walunj, P. R.; Kishor, P. B. Kavi; Nikam, Tukaram D.

doi:10.1007/s11738-013-1233-x

Cited by 36 publications

(23 citation statements)

References 78 publications

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“…These authors observed that tomato cultivars grown in vitro under salt stress showed an increase in leaf proline content, with maximum peaks observed with 160 mM NaCl. Increased proline content was also found in species grown in vitro under salt stress such as Populus euphratica (Watanabe et al 2000), tomato and cauliflower (Wahid et al, 2014), and Bacopa monnieri (Ahire et al, 2013). Miranda et al (2014) observed that leaves of P. peruviana showed higher proline levels as the exposure time of the plants to NaCl increased.…”

Section: Discussionmentioning

confidence: 90%

Effects of silicon on antioxidant enzymes, CO2, proline and biological activity of in vitro-grown cape gooseberry under salinity stress

Rezende¹,

Soares²,

Santos³

et al. 2017

Aust J Crop Sci

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Cape gooseberry (Physalis peruviana L.) cannot tolerate high levels of salinity. Salt stress is one of the most damaging abiotic stresses that affects plant development. However, there are some evidences that silicon (Si) can act as a mitigating agent of environmental stresses. Towards understanding the stress using tissue culture, we investigated the effect of in vitro NaCl-induced salt stress in cape gooseberry and the potential mitigating effect provided by Si. Nodal segments were inoculated in MS medium with different combinations of NaCl concentrations (0.5 and 1.0% NaCl) and silicic acid (0.0, 0.5, and 1.0 g L -1 ). The experimental design was completely randomized, in a factorial design with a control treatment [(2x3) + 1], totalizing 7 treatments. The control treatment consisted of an additional treatment without the addition of any of the factors. Isoenzyme activity, proline content, CO 2 level and biological activity were assessed after 30 d. We found an increase in all antioxidant enzymes (SOD, CAT and POD) activities when salt stress was imposed, indicating that the plant has an efficient system to protect itself against stress. Among these enzymes, Si played a mitigating role only in POD activity. In relation to other evaluated enzymes, NaCl enhanced the EST and MDH activities, followed by a subsequent decrease in MDH. Si showed different performances according to the concentration of NaCl. Electrophoretic analysis represented by bands illustrated these behaviors. Proline content increased as salinity increased, and Si effect was observed by increasing the levels of this amino acid. High CO 2 level was found at the concentration of 1.0% NaCl, and Si contributed to decrease this variable to normal levels. The reduction in the respiratory rates may contribute to the allocation of carbon to other chemical reactions such as the synthesis of new tissues. Salt stress also increased the biological activity of leaves and there was no effect of Si for this variable. We conclude that salt stress causes a great damage to in vitro-grown cape gooseberry plants and the addition of 1.0 g L -1 Si can ameliorate that damage for some characteristics. However, more studies are necessary since the physiological changes played by Si have been poorly understood for dicots.

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Section: Discussionmentioning

confidence: 90%

Effects of silicon on antioxidant enzymes, CO2, proline and biological activity of in vitro-grown cape gooseberry under salinity stress

Rezende¹,

Soares²,

Santos³

et al. 2017

Aust J Crop Sci

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“…The higher accumulation of proline in salinity-stressed plants at elevated CO 2 in Bermudagrass in this study might be involved in elevated CO 2 -induced improvement in salinity tolerance, although the mechanism of its increases under elevated CO 2 is unknown. Salinity stress also causes the accumulation of glycine betaine at ambient CO 2 concentration in various plant species, such as Suaeda maritima (Genard et al, 1991), Bacopa monnieri (Ahire et al, 2013), Triticum aestivum (Liang et al, 2009), and Salicornia europaea (Moghaieb et al, 2004). However, the accumulation of GB associated with elevated CO 2 under salinity stress has not been well documented, and has been reported in only a few cases (Han et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Physiological factors involved in positive effects of elevated carbon dioxide concentration on Bermudagrass tolerance to salinity stress

Sun

Fan

et al. 2015

Environmental and Experimental Botany

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“…The lowest accumulation rate was estimated in the biomass from the medium with addition of L-tryptophan (15.5 mg/g DW). However, even this value is much higher than in the case of the application of methyl jasmonate (8.81 mg/g DW bacosides) (Sharma et al 2013) or sodium chloride (1.32 mg/g DW bacosides) (Ahire et al 2013) into the medium for B. monnieri in vitro culture. For the combination of methyl jasmonate and salicylic acid, the content of bacoside A in biomass of B. monnieri was 59.18 mg/g DW (Largia et al 2015).…”

Section: Bacosidesmentioning

confidence: 82%

“…It was found that MS medium without growth regulators did not determine optimal growth. According to other researchers (Ceasar et al 2010;Ahire et al 2013), optimal growth is obtained on MS medium supplemented with growth regulators such as BAP and NAA. The increases in biomass are shown in Figure 1.…”

Section: Resultsmentioning

confidence: 99%

In vitrocultures ofBacopa monnieriand an analysis of selected groups of biologically active metabolites in their biomass

Muszyńska

Łojewski

Sułkowska-Ziaja

et al. 2016

Pharmaceutical Biology

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Context: Bacopa monnieri L. Pennell (Scrophulariaceae) is one of the most important plants in the system of Indian medicine (Ayurveda). Objective: This paper studies the optimal growth of B. monnieri for effective accumulation of metabolites. Biomass growth of this plant could be accomplished in liquid cultures on Murashige & Skoog medium. Materials and methods: Powdered shoots of in vitro cultures of B. monnieri were extracted by methanol for indole compounds, phenolic compounds and bacosides for RP-HPLC analysis. Fatty acid analysis was performed via gas chromatography. Anti-inflammatory effect of B. monnieri extracts was evaluated in the A549 cells. COX-2 and cPGES expression was analyzed using Western blots. Results: L-Tryptophan and serotonin were found in biomass from in vitro cultures of B. monnieri on MS medium and in biomass from the MS mediums enriched with the different additions such as of 0.1 g/L magnesium sulphate, 0.1 g/L zinc hydroaspartate, 0.1 g/L L-tryptophan, 0.25 g/L serine, 0.5 g/L serine and 0.5 mg/L anthranilic acid. The content of L-tryptophan and serotonin compounds was significant in biomass from medium with the addition of 0.1 g/L zinc hydroaspartate (0.72 mg/g dry weight and 1.19, respectively). Phenolic compounds identified in biomass from the same variants of MS medium were chlorogenic acid (ranging from 0.20 to 0.70 mg/g dry weight), neochlorogenic acid (ranging from 0.11 to 0.40 mg/g dry weight) and caffeic acid (ranging from 0.01 to 0.04 mg/g dry weight). The main group of fatty acids in biomass was saturated fatty acids (53.4%). The predominant fatty acid was palmitic acid. A significant decrease of COX-2 and cPGES expression was observed in the A549 cells activated with LPS and treated with B. monnieri extracts. Discussion and conclusions: As far as we know, this is the first analysis of indole compounds and phenolic acids in this plant. The multi-therapeutic effect of B. monnieri is expressed by the activity of bacosides. Information about the presence of indole and phenolic compounds, and fatty acids in this plant is limited, but the content of these compounds might participate in the physiological activity of B. monnieri. ARTICLE HISTORY

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Effect of sodium chloride-induced stress on growth, proline, glycine betaine accumulation, antioxidative defence and bacoside A content in in vitro regenerated shoots of Bacopa monnieri (L.) Pennell

Cited by 36 publications

References 78 publications

Effects of silicon on antioxidant enzymes, CO2, proline and biological activity of in vitro-grown cape gooseberry under salinity stress

Effects of silicon on antioxidant enzymes, CO2, proline and biological activity of in vitro-grown cape gooseberry under salinity stress

Physiological factors involved in positive effects of elevated carbon dioxide concentration on Bermudagrass tolerance to salinity stress

In vitrocultures ofBacopa monnieriand an analysis of selected groups of biologically active metabolites in their biomass

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