EFFECT OF SINGLE ASD REPEATED DOSES OF GOSSYPOL OK THE RAT<sup>1</sup>

Eagle, Edward; Bialer, Henry F.

doi:10.1111/j.1365-2621.1950.tb16470.x

Cited by 21 publications

(9 citation statements)

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“…Autopsies on deceased rats revealed either severe affections of lung, liver, kidney and gastrointestinal tract or the apparently normal status of the organs. Very similar organ damages were numerously reported from rats that had died during gossypol treatment (Eagle and Bialek -1950;El-Nockrashy et al -1963;Abou-Donia -1980). It is assumed that gossypol, besides other effects, might interfere with the availability of free iron (which then is bound to gossypol) and promote nitrogen loss via urinary excretion leading to edema and eventually to death (Bressani -1980).…”

Section: Discussionsupporting

confidence: 72%

Toxicity of Gossypol at Antifertility Dosages in Male Rats: Statistical Analysis of Lethal Rates and Body Weight Responses

Weinbauer

Rovan

Frick³

2009

Andrologia

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Section: Discussionsupporting

confidence: 72%

Toxicity of Gossypol at Antifertility Dosages in Male Rats: Statistical Analysis of Lethal Rates and Body Weight Responses

Weinbauer

Rovan

Frick³

2009

Andrologia

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“…All 3 samples of cottonseed pigment glands, however, despite their low gossypol content, were more toxic to the rat than any of the 5 samples of gossypol combination products containing 7.2 to 68.6% gossypol (A, B, C, D, F) or the sample of pure gossypol ( E ) . These data offer further evidence of lack of correlation between analyzed gossypol content and acute oral toxicity as was originally pointed out for the rat by Eagle et al (3,4,5 ) and subsequently confirmed by Ambrose and Robbins (1).…”

Section: 2supporting

confidence: 78%

Toxicity and Body Weight‐depressing Effects in the Rat of Water‐soluble Combination Products of Gossypol, Gossypol, and Cottonseed Pigment Glands

Eagle

Bialek

1952

Journal of Food Science

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I n a recent paper Castillon and Altschul ( 2 ) described experiments on the toxicity of some new water-soluble combination products of gossypol to aquarium fish. The present investigation deals with the acute oral toxicity and effects of the body weight of the rat of identical and similar combinations of gossypol with glycine, dextrose, and peanut protein. The results of incorporating in the diet various levels of pure gossypol and cottonseed pigment glands are also reported. An understanding of the physiological effects bears direct application to the improvement of the food of man and animals. METHODSSamples of untreated cottonseed pigment glands, pure gossypol, and combination products with gossypol were prepared as described (8,5 ) . Individually caged male rats (150-220 g.) were fasted for 18 hours, with water ad libitum, in an air-conditioned animal room maintained a t 80" 2 1°F. and ca. 45% relative humidity. Single doses of the test materials were administered by stomach tube as 10% solutions or suspensions in distilled water. All rats had free access to stock diet and water after intubation. The per cent composition of the control and basal ration was: whole ground wheat 21.0, meat and bone scraps 18.0, skim milk 15.0, soybean oil meal 15.0, yellow corn 13.0, lard 10.0, yeast and salt mixture 2.5, liver and glandular meal 2.0, alfalfa leaf meal 2.0, wheat germ oil 1.0, vitamin A and II oil (2250 U.S.P. A, 300 U.S.P. Ds per g.) 0.5. The surviving rats were weighed daily or 3 times per week for periods of 2 weeks or more. Calculation of the LDso value was made after 1 week by the method of Reed and Muench (7). All gossypol analyses reported in this study were made a t the Southern Regional Laboratory by the method of Pons and Guthrie (6). I n many cases 4 or more individual analyses were made and the average of these is shown as the gossypol content.I n the 4 subacute toxicity experiments, levels of 0, 0.05, 0.075, 0.10, 0.15, 0.20, 0.30, 0.40, 0.50, 0.536, and 1.34 g. of pure gossypol and levels of 1.34 g. of cottonseed pigment glands containing 21.85% gossypol and 0.6 and 0.9 g. of cottonseed pigment glands containing 33.22% gossypol were added per 100 g. of stork ration and fed ad Zibtfum to weanling or young adult male rats. These rats were weighed daily for the first 14 days, and at least thrice weekly thereafter. RESULTSThe samples of gossypol, untreated cottonseed pigment glands, and gossypol combination products are listed in Table 1 in the order of increasing acute oral toxicity. The LD,, value recorded for pure gossypol administered as an aqueous suspension was not significantly different from the LD,, value for pure gossypol dissolved in soybean oil. There was poor correlation between the gossypol content of the various samples and their LD,, values. For example, Sample F, which contained 68.6% extractable gossypol, was more toxic than 100% gossypol, and Sample D, containing 543 TABLE 1

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“…In rats these include intestinal dilation and impaction, hemorrhagic congestion of the gastrointestinal tract, pulmonary and renal congestion, and degeneration of the seminiferous tubules. The LD50 value for gossypol in rats is between 2500 and 3300 mg/kg [3]. Of the domestic species, swine appear to be more sensitive than others in the development of toxic signs.…”

Section: Discussionmentioning

confidence: 99%

Absence of Histopathology in Somatic Tissues of Rats Made Infertile with Gossypol

Engler

Hess

Hoffer

1984

Archives of Andrology

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Although the effect of gossypol on the testis and epididymis has been well documented, its effect on the somatic tissues of animals made infertile with the drug has been less well studied. In this study rats were treated daily with gossypol at either 20 mg/kg or 30 mg/kg for 6 weeks and at 10 mg/kg for 7 months. Complete tissue sets from control and gossypol-treated rats, including 26 organs, were subjected to histological examination. No significant histopathological differences were noted at the light microscope level between the control and infertile groups.

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EFFECT OF SINGLE ASD REPEATED DOSES OF GOSSYPOL OK THE RAT¹

Cited by 21 publications

References 5 publications

Toxicity of Gossypol at Antifertility Dosages in Male Rats: Statistical Analysis of Lethal Rates and Body Weight Responses

Toxicity of Gossypol at Antifertility Dosages in Male Rats: Statistical Analysis of Lethal Rates and Body Weight Responses

Toxicity and Body Weight‐depressing Effects in the Rat of Water‐soluble Combination Products of Gossypol, Gossypol, and Cottonseed Pigment Glands

Absence of Histopathology in Somatic Tissues of Rats Made Infertile with Gossypol

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EFFECT OF SINGLE ASD REPEATED DOSES OF GOSSYPOL OK THE RAT1

Cited by 21 publications

References 5 publications

Toxicity of Gossypol at Antifertility Dosages in Male Rats: Statistical Analysis of Lethal Rates and Body Weight Responses

Toxicity of Gossypol at Antifertility Dosages in Male Rats: Statistical Analysis of Lethal Rates and Body Weight Responses

Toxicity and Body Weight‐depressing Effects in the Rat of Water‐soluble Combination Products of Gossypol, Gossypol, and Cottonseed Pigment Glands

Absence of Histopathology in Somatic Tissues of Rats Made Infertile with Gossypol

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EFFECT OF SINGLE ASD REPEATED DOSES OF GOSSYPOL OK THE RAT¹