Effect of silymarin alcoholic extract on surgery‐induced intraperitoneal adhesion in rats

Abstract

Aim: Adhesion formation is the greatest complication in abdominal surgery. Silybum marianum (S. marianum) extract contains phenolic compounds whit antioxidant activities, thus could reduce adhesion formation. The aim of the present study was to examine its effect on intraabdominal adhesion. Patients and Methods: Thirty male Wistar rats were randomly divided into three groups. Groups A and B received 1 per cent and 5 per cent concentrations of the S. marianum extract, and group C (control group) received distilled water. After anaesthesia was administered, the abdominal wall was opened and three shallow, longitudinal and transverse incisions (2 cm in length) were made on the right wall of the abdomen. A 2 × 2 cm piece was removed from the peritoneal surface on the left side of the abdominal wall; 3 mL of S. marianum extract or distilled water was then administered into the abdominal cavity. Adhesion degrees were determined according to the Canbaz scale. Histopathological examination was also determined according to the severity of fibrosis and inflammation. Data analysis was performed using SPSS version16. Results: There was significant difference in the adhesion formation between the groups (P = 0.023). The adhesion degree in groups A and B were significantly lower than that of the control (P < 0.05). In the histopathological examination, significant differences were observed between the control and extract-treated groups in term of fibrosis and inflammation (P < 0.05). Conclusion: S. marianum extract has preventive effects on post-laparotomy intraabdominal adhesion. Therefore, through further clinical studies, S. marianum extract and its derived compounds might be used in humans for the treatment of these complications.

Effects of Silymarin Supplementation on Leptin, Adiponectin and Paraoxanase Levels and Body Composition During Exercise: A Randomized Double-Blind Placebo Controlled Clinical Trial

“…The extract was concentrated using vacuum distillation until the volume was reduced to 20 mL. The concentrated extract was dried (18). The placebo capsules consisted of dextrose.…”

Salvia miltiorrhiza hydroalcoholic extract inhibits postoperative peritoneal adhesions in rats

“…Various methods are used to create the model of peritoneal adhesion in rats, for instance intestinal abrasion, large intestine anastomosis, ischemic buttons, and peritoneal incisions and removing a piece of the peritoneum [ 41 ]. In the current research, we employed the method of the peritoneal wall incision and removing a piece of peritoneum to induce adhesions, which is known to be one of the acceptable methods [ 42 – 44 ].…”

Application of Traditional Chinese Medicines in Postoperative Abdominal Adhesion

“…Silymarin has anti-inflammatory, antifibrotic, and antioxidant properties [58,59]. Previous studies have shown that 3 ml, 1% or 5% concentration, of the silymarin extract was administered into the abdominal cavity for 14 days, which is effective to prevent PAA [60,61]. It may be attributed to the inhibition of collagen overproduction and accumulation, and complete lysis of fibrin in the experimental adhesion model of rats.…”