Effect of silver nanoparticles in the induction of apoptosis on human hepatocellular carcinoma (HepG2) cell line

Ahmadian, Elham; Dizaj, Solmaz Maleki; Rahimpour, Elaheh; Hasanzadeh, Amir; Eftekhari, Aziz; zadegan, Hasan Hosain; Halajzadeh, Jamal; Ahmadian, Hale

doi:10.1016/j.msec.2018.08.027

Cited by 111 publications

(54 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ahmadian et al [52] showed that chemically produced AgNPs have an IC 50 value of 75 µg/mL against the HepG2 cell line. In contrast, Justin Packia Jacob et al [53] estimated an IC 50 of 31.25 µg/mL against HepG2 cells for AgNPs prepared by reduction using Piper longum leaf extracts and hypothesized that this action might be attributed to piperidine present in the leaf extract coating the AgNP surface.…”

Section: Resultsmentioning

confidence: 99%

Biosynthesis of Silver Nanoparticles Mediated by Extracellular Pigment from Talaromyces purpurogenus and Their Biomedical Applications

et al. 2019

View full text Add to dashboard Cite

In recent years, green syntheses have been researched comprehensively to develop inexpensive and eco-friendly approaches for the generation of nanoparticles. In this context, plant and microbial sources are being examined to discover potential reducing agents. This study aims to utilize an extracellular pigment produced by Talaromyces purpurogenus as a prospective reducing agent to synthesize silver nanoparticles (AgNPs). Biosynthesized AgNPs were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), electron probe micro analyser (EPMA), and zeta potential. The pigment functional groups involved in the generation of AgNPs were investigated using Fourier transform infrared spectroscopy. TEM images showed that the generated nanoparticles were spherical, hexagonal, rod-shaped, and triangular-shaped with a particle size distribution from 4 to 41 nm and exhibited a surface plasmon resonance at around 410 nm. DLS and zeta potential studies revealed that the particles were polydispersed and stable (−24.8 mV). EPMA confirmed the presence of elemental silver in the samples. Biosynthesized AgNPs exhibited minimum inhibitory concentrations of 32 and 4 μg/mL against E. coli and S. epidermidis, respectively. Further, cytotoxicity of the AgNPs was investigated against human cervical cancer (HeLa), human liver cancer (HepG2), and human embryonic kidney (HEK-293) cell lines using 5-fluorouracil as a positive control. A significant activity was recorded against HepG2 cell line with a half-maximal inhibitory concentration of 11.1 μg/mL.

show abstract

Section: Resultsmentioning

confidence: 99%

Biosynthesis of Silver Nanoparticles Mediated by Extracellular Pigment from Talaromyces purpurogenus and Their Biomedical Applications

et al. 2019

View full text Add to dashboard Cite

show abstract

“…AgNPs have been observed to exhibit good anticancer activities in breast cancer 158 , cervical cancer 208 , colon cancer 209 , ovarian cancer 210 , pancreatic ductal adenocarcinoma 211 , lung cancer 212 , hepatocellular carcinoma 213 , melanoma 214 , osteosarcoma 215 , etc. ( Table 5 ).…”

Section: Medical Applications Of Agnpsmentioning

confidence: 99%

Silver nanoparticles: Synthesis, medical applications and biosafety

et al. 2020

View full text Add to dashboard Cite

Silver nanoparticles (AgNPs) have been one of the most attractive nanomaterials in biomedicine due to their unique physicochemical properties. In this paper, we review the state-of-the-art advances of AgNPs in the synthesis methods, medical applications and biosafety of AgNPs. The synthesis methods of AgNPs include physical, chemical and biological routes. AgNPs are mainly used for antimicrobial and anticancer therapy, and also applied in the promotion of wound repair and bone healing, or as the vaccine adjuvant, anti-diabetic agent and biosensors. This review also summarizes the biological action mechanisms of AgNPs, which mainly involve the release of silver ions (Ag + ), generation of reactive oxygen species (ROS), destruction of membrane structure. Despite these therapeutic benefits, their biological safety problems such as potential toxicity on cells, tissue, and organs should be paid enough attention. Besides, we briefly introduce a new type of Ag particles smaller than AgNPs, silver Ångstrom (Å, 1 Å = 0.1 nm) particles (AgÅPs), which exhibit better biological activity and lower toxicity compared with AgNPs. Finally, we conclude the current challenges and point out the future development direction of AgNPs.

show abstract

“…Moreover, we get the core proteins of interests, Casp3 and Bcl2l1 from PPI network. Casp3 is closely related to apoptosis and plays critical role in apoptotic pathway [42]. One of the earliest changes of apoptosis is characterized by the emergence of a series of proteases -Caspases.…”

Section: Functional Analysis Of Candidate Targetsmentioning

confidence: 99%

Investigating the pharmacological mechanisms of SheXiang XinTongNing against coronary heart disease based on network pharmacology

Jia

Cao³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background SheXiang XinTongNing, a commercially available Chinese patent medicine, has been widely used in the treatment of coronary heart disease. However, the mechanisms of SheXiang XinTongNing are still unclear. The aim of this study was to investigate the pharmacological mechanisms of SheXiang XinTongNing against coronary heart disease via network analysis. Method The traditional Chinese medicine system pharmacology analysis platform was used to screen the potential active constituents of the six traditional Chinese medicines in SheXiang XinTongNing, and the potential targets were obtained from PharmMapper. The genome annotation database platform was used to screen the candidate targets related to coronary heart disease. Then the drug-components-targets network and protein interaction network were built by Cytoscape 3.6.0 software. Further, GO bio-functional enrichment analysis and KEGG pathway enrichment analysis were performed through annotation, visualization and integrated discovery database. Results Results showed that the drugs-components-targets network contains 104 targets and 62 key components. The protein interaction network consisted of 107 nodes; key targets included Bcl2l1, IGF1, SRC, CASP3, et al. Functionally, the candidate targets were significantly associated with multiple pathways such as PI3K-Akt signaling pathway, MAPK signaling pathway, Ras signaling pathway, FoxO signaling pathway, Endocrine resistance. Given the above, the pharmacological activities of SheXiang XinTongNing may be predominantly related to several factors such as cell apoptosis, inflammation and angiogenesis. Conclusion XTN can effectively attenuate the symptoms of coronary heart disease through diverse pathways. The research proves that network pharmacology can successfully reveal the mechanisms of traditional Chinese medicine in a holistic view. Our systematic analysis lays a foundation for further studying.

show abstract

Effect of silver nanoparticles in the induction of apoptosis on human hepatocellular carcinoma (HepG2) cell line

Cited by 111 publications

References 44 publications

Biosynthesis of Silver Nanoparticles Mediated by Extracellular Pigment from Talaromyces purpurogenus and Their Biomedical Applications

Biosynthesis of Silver Nanoparticles Mediated by Extracellular Pigment from Talaromyces purpurogenus and Their Biomedical Applications

Silver nanoparticles: Synthesis, medical applications and biosafety

Investigating the pharmacological mechanisms of SheXiang XinTongNing against coronary heart disease based on network pharmacology

Contact Info

Product

Resources

About