Effect of silver-containing agents on the ultra-structural morphology of dentinal collagen

Sayed, Mahmoud; Hiraishi, Noriko; Matin, Khairul; Abdou, Ahmed; Burrow, Michael F.; Tagami, Junji

doi:10.1016/j.dental.2020.04.028

Cited by 25 publications

(29 citation statements)

References 38 publications

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“…Following the method used by Sayed et al, Streptococcus mutans MT8148 (S. mutans) was used after freshly preparing a suspension (described below) to incubate the samples. After brushing procedures, each specimen was immersed in 150 μl of bacterial suspension in a separate well of a sterile 96-well flat-bottom culture plate 19 .…”

Section: Sem/eds Analysis Observationmentioning

confidence: 99%

“…After that, the suspensions were used directly after placing 150 μl aliquots into each well of the 96-well culture plate, and incubated at 37 °C for 2 h. All the specimens were transferred into separate dark-colored microtubes for performing the bacterial viability test. The remaining suspensions of the S. mutans were used for colony-formation test and growth curve test (OD 490 ) as mentioned by Sayed et al 19 . All the following tests were performed by the same operator who was blinded to the active ingredient of the experimental groups.…”

Section: Sem/eds Analysis Observationmentioning

confidence: 99%

“…The excitation wavelengths for the dyes were approximately 480/530 nm for SYTO-9 (greensignals) and 520/580 nm for propidium-iodide (red-signals). Bacterial cells viability was evaluated using a fluorescence microscope (FM, CKX41; Olympus, Tokyo, Japan) as mentioned by Sayed et al 19 .…”

Section: Sem/eds Analysis Observationmentioning

confidence: 99%

“…Then, they were plated in petri-dishes containing Mitis Salivarius agar medium. After 48-h incubation at 37 °C under anaerobic conditions, the number of CFU/ml was counted using an optical microscope as mentioned by Sayed et al 19 . www.nature.com/scientificreports/ Growth curve of the bacteria (OD 490 ).…”

Section: Sem/eds Analysis Observationmentioning

confidence: 99%

“…After removing the specimens from the bacterial suspension, 150 μl of an autoclaved solution of BHI was added to each well that contains the bacterial suspensions and incubated at 37 °C. Then incubated at 37 °C optical density was immediately measured as baseline and then continued to be measured hourly for 8 h using the same spectrophotometer at 490 nm as mentioned by Sayed et al 19 .…”

Section: Sem/eds Analysis Observationmentioning

confidence: 99%

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Effects of mechanical abrasion challenge on sound and demineralized dentin surfaces treated with SDF

Sayed

Tsuda

Matin

et al. 2020

Sci Rep

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This study evaluated the effect of mechanical abrasion on the surface integrity, color change (ΔE) and antibacterial properties of demineralized and sound dentin surfaces treated with silver-diammine-fluoride (SDF). The dentin specimens were divided into two groups: sound and demineralized dentin, then divided into three sub-groups, control (no-treatment), SDF, and SDF + potassium-iodide (KI). Each sub-group was further divided into two groups, one exposed to mechanical brushing and the other without brushing. Specimens were analyzed for the ΔE, surface roughness/surface loss and antibacterial properties (CFU, optical density and fluorescent microscope). Repeated Measures ANOVA was used for statistical analysis of color change while one-way ANOVA was used for CFU analysis. SDF and SDI + KI groups showed significant reduction in ΔE with brushing in the sound dentin group unlike the demineralized group. The surface roughness values were higher for both SDF and SDF + KI groups but roughness values significantly decreased after brushing. Both SDF and SDF + KI groups revealed significantly less surface loss than control. The SDF group showed high anti-bacterial effect after brushing, unlike SDF + KI group. So, we concluded that mechanical brushing improved the esthetic outcome. While, SDF and SDF + KI could protect the dentin surface integrity. SDF-treated dentin possesses an antibacterial property even after mechanical brushing.

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Section: Sem/eds Analysis Observationmentioning

confidence: 99%

Section: Sem/eds Analysis Observationmentioning

confidence: 99%

Section: Sem/eds Analysis Observationmentioning

confidence: 99%

Section: Sem/eds Analysis Observationmentioning

confidence: 99%

Section: Sem/eds Analysis Observationmentioning

confidence: 99%

See 3 more Smart Citations

Effects of mechanical abrasion challenge on sound and demineralized dentin surfaces treated with SDF

Sayed

Tsuda

Matin

et al. 2020

Sci Rep

Self Cite

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Which is Better for Nanomedicines: Nanocatalysts or Single‐Atom Catalysts?

Zhao

Zhang

Yang

et al. 2020

Adv Healthcare Materials

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With the rapid advancements in nanotechnology and materials science, numerous nanomaterials have been used as catalysts for nanomedical applications. Their design and modification according to the microenvironment of diseases have been shown to achieve effective treatment. Chemists are in pursuit of nanocatalysts that are more efficient, controllable, and less toxic by developing innovative synthetic technologies and improving existing ones. Recently, single-atom catalysts (SACs) with excellent catalytic activity and high selectivity have attracted increasing attention because of their accurate design as nanomaterials at the atomic level, thereby highlighting their potential for nanomedical applications. In this review, the recent advances in nanocatalysts and SACs are briefly summarized according to their synthesis, characterizations, catalytic mechanisms, and nanomedical applications. The opportunities and future scope for their development and the issues and challenges for their application as nanomedicine are also discussed. As far as it is known, the review is the systematic comparison of nanocatalysts and SACs, especially in the field of nanomedicine, which has promoted the development of nanocatalytic medicine.

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Applicability of silver nanoparticles and innovation of magnetic nanoparticles in dentistry

Rosa

Narciso

Nunes

et al. 2022

Fundamentals and Industrial Applications of Magnetic Nanoparticles

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Effect of silver-containing agents on the ultra-structural morphology of dentinal collagen

Cited by 25 publications

References 38 publications

Effects of mechanical abrasion challenge on sound and demineralized dentin surfaces treated with SDF

Effects of mechanical abrasion challenge on sound and demineralized dentin surfaces treated with SDF

Which is Better for Nanomedicines: Nanocatalysts or Single‐Atom Catalysts?

Applicability of silver nanoparticles and innovation of magnetic nanoparticles in dentistry

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