Effect of silk fibroin nanofibers containing silver sulfadiazine on wound healing

Abstract

Background One of the promising applications of silk fibroin (SF) in biomedical engineering is its use as a scaffolding material for skin regeneration. The purpose of this study was to determine the wound healing effect of SF nanofibrous matrices containing silver sulfadiazine (SSD) wound dressings. Methods An SF nanofibrous matrix containing SSD was prepared by electrospinning. The cell attachment and spreading of normal human epidermal keratinocytes (NHEK) and normal human epidermal fibroblasts (NHEF) to SF nanofibers containing three different concentrations of SSD contents (0.1, 0.5, and 1.0 wt%) were determined. In addition, a rat wound model was used in this study to determine the wound healing effect of SF nanofibers containing SSD compared with that of Acticoat™, a commercially available wound dressing. Results The number of NHEK and NHEF attached to SF nanofibers containing SSD decreased when the concentration of SSD increased. The number of attached NHEF cells was lower than that of attached NHEK cells. The SF matrix with 1.0 wt% SSD produced faster wound healing than Acticoat, although 1.0 wt% SSD inhibited the attachment of epidermal cells to SF nanofibers in vitro. Conclusion The cytotoxic effects of SF nanofibers with SSD should be considered in the development of silver-release dressings for wound healing through its antimicrobial activity. It is challenging to design wound dressings that maximize antimicrobial activity and minimize cellular toxicity.

Biomaterial-Based Scaffolds as Antibacterial Suture Materials

“…RSF-BAMG and RSF/HACC-BAMG scaffolds were fabricated by electrospinning, as shown in Figure . Dry BAMG was wet using phosphate-buffered saline (PBS) to improve its conductivity and then stretched smoothly onto aluminum foil to collect the electrospun fibers, significantly different from other electrospun RSF scaffolds, , that promoted improvement in its mechanical properties. It should be noted that the BAMG exhibited entirely distinct morphologies on different surfaces: a smooth serosal surface and a rough muscular surface with large numbers of pores (Figure ), providing the potential for increasing interfacial interactions.…”

Current status and future outlook of nano‐based systems for burn wound management

“…The rate of wound closing was faster and the number of NHEK and NHEF attached to the SF nanofibers containing SSD were decreased (Jeong, Kim et al, 2014).…”

Silk Sponges with Surface Antimicrobial Activity

“…). , While silkworm silk can easily be harvested from the cocoons, spider silk is mainly produced via bioengineering technology due to the wild nature of spiders. Several studies have described the preparation of antimicrobial silk scaffolds by bulk-loading and surface functionalization methods, for examples, the bulk loading of gentamicin into silk sponges, ampicillin into films, silver sulfadiazine into porous films and an electrospun matrix, , and silver nanoparticles into silk fibers. − Meanwhile, surface functionalization approaches include coating of erythromycin on silk sponges and silver sulfadiazine on electrospun silk mats, , covalent conjugation of antimicrobial peptide on a silk nanofiber membrane, and layer-by-layer (LbL) assembly of lysozyme on a silk membrane …”