Combined with the design of a bridge with prestressed concrete continuous box-girder, and in accordance with the Chinese guidelines of seismic design for highway bridge, the numerical models with and without considering the pile-soil action were built using Midas Civil software. The response spectrum method was used to analyze the seismic response of the bridge under E1 and E2 seismic actions. The vibration characteristics such as frequency, period and mode as well as the internal forces and displacements of piers are discussed in view of the effect of the pile-soil action.