Effect of shock waves on suspended and immobilized L1210 cells

Brümmer, Franz; Brenner, Joachim; Bräuner, Thomas; Hülser, Dieter F.

doi:10.1016/0301-5629(89)90067-7

Cited by 79 publications

(26 citation statements)

References 27 publications

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“…This discrepancy indicates the need for a further character- ization of the mechanism of shock wave induced cytotoxicity. Our earlier studies on the effect of shock waves on L I210 mouse leukemia cell suspensions (Briimmer et al 1989) revealed a dose-dependent decrease in the number of intact cells with a 50%-loss of cells after application of 500 SW. In these experi-ments, however, the population of intact cells contained subpopulations of nonviable cells as determined with a double staining technique and flow cytometry.…”

Section: Discussionmentioning

confidence: 94%

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Histopathology of shock wave treated tumor cell suspensions and multicell tumor spheroids

Bräuner

Brümmer

Hülser

1989

Ultrasound in Medicine & Biology

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Section: Discussionmentioning

confidence: 94%

“…2b. Since the LDs0 for L I210 cells is about 500 SW (Briimmer et al 1989), it is not surprising that many cells could not be distinguished from untreated cells.…”

Section: Resultsmentioning

confidence: 99%

Histopathology of shock wave treated tumor cell suspensions and multicell tumor spheroids

Bräuner

Brümmer

Hülser

1989

Ultrasound in Medicine & Biology

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“…Viability of cells was determined by combined measurements with a cell counter (Coulter Counter D Industrial, Coulter Electronics, Hialeah, FL, USA) excluding destroyed cells, and a flow cytometer (FACS-Analyzer, Becton-Dickinson, Heidelberg, Germany) using a double staining method for viable and dead cells (Jones and Senft 1985): cells that were able to hydrolyze fluorescein diacetate (FDA) and so exhibit a green fluorescence of fluorescein were considered as viable, while cells whose membrane could not exclude the dye propidium iodide and show a red fluorescence were counted as dead (for details, see Brtimmer et al 1989). Determinations of cell diameters were performed in a FACS-Analyzer, equipped with an electrical resistance pulse sizing following the Coulter principle (Coulter 1956), and using a calibration curve for absolute particle sizes (Brenner and Htilser 1991 ).…”

Section: Methodsmentioning

confidence: 99%

Cavitation-generated free radicals during shock wave exposure: Investigations with cell-free solutions and suspended cells

Suhr

Brümmer

Hülser

1991

Ultrasound in Medicine & Biology

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Abstract--Extracorporeally generated shock waves as used in lithotripsy of urinary and biliary stones exhibit side effects in vivo. Furthermore, these shock waves destroy eukaryotic cells during in vitro treatment in suspension. A possible cause of these damaging effects might be cavitation, the growth and collapse of bubbles in liquids exposed to tensile stresses. During the collapse, temperature inside these cavitation bubbles rises up to several thousand K, leading to the formation of free radicals. We demonstrated the occurrence of cavitation-generated free radicals by direct reaction with fluorescent dyes in solution after shock wave treatment and investigated the resulting cell killing by variation of the cellular antioxidative defense status. We present evidence for the generation of intracellular free radicals during shock wave treatment of suspended cells.

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“…In vitro, these secondary effects can be avoided by the immobilization of single cells or multicell spheroids in gelatin (see Figs. 3 f and 4b, d, f) [9,13]. Unter this condition, significant dose-dependent cellular damage was no longer detectable using flow cytometric techniques (Fig.…”

Section: Cell Culturesmentioning

confidence: 99%

“…We have performed some experiments on suspended and immobilized cell cultures that enable a separation of the primary and secondary effects of shock-wave treatment [8,9,12,13,41]. L 1210 cells (lymphocytic mouse leukemia) have been treated as single-cell suspensions, whereas the human cervical carcinoma HeLa as well as the mouse mammary carcinoma EMT6/Ro were exposed to shock waves as three-dimensionally grown mukicellular spheroids.…”

Section: Cell Culturesmentioning

confidence: 99%

Biological effects of shock waves

1990

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Summary.Extracorporeal shock wave lithotripsy has become established worldwide as the method of choice for the treatment of nephrolithiasis and ureterolithiasis over the last 10 years. Although initial studies showed no damaging effects of the shock waves on organs and tissues, numerous recent reports have presented evidence for severe acute effects and chronic complications after shock wave treatment. The pathophysiological effects on kidneys and the histopathological effects on organs or tissues in man and animal, and also the effects on ceils in culture and tumors are sumarized. Suspended and immobilized cell cultures were used to characterize and quantify the efficacy of shock wave. Extended applications of shock waves and possible modifications to shock wave generators are discussed.A reproducible generation of shock waves in fluids was first reported by Eisenmenger [31,32], who described an electromagnetic arrangement using a flat solenoid and a metal membrane. About 20 years later, underwater sparkgap-induced shock waves were used for kidney stone disintegration [17], a procedure that has become clinical routine [30]. In the meantime, the third generation of lithotripters has been developed (Siemens Lithostar Plus, Dornier Compact, Storz Modulith). Most of them are equipped with electromagnetic shock-wave emitters (EMSE), but piezo-electric shock-wave sources (Piezolith 2500, Diasonics) with similar properties for stone fragmentation are also in clinical use. However, all commercially available shock-wave generators (spark gap, electromagnetic, piezo-electric) produce side effects that accompany stone disintegration in patients. Furthermore, their biological effects are described as injuries to organs or tissues in vivo that have been exposed to the focal area of shock waves and as damage to cells in culture that have been treated with shock waves. * Dedicated to Prof. Dr. Wolfgang Eisenmenger on the occasion of his 60th birthday Side effectsIn kidney-or gallstone treatment with shock waves, side effects are equivocally classified and certainly depend not only on the number and energy of the applied shock waves but also on the disposition of the patients. Petechial bleeding of the skin that can be observed macroscopically has been found in about 10% of patients [29]. Varying degrees of subcapsular fluid collection and hemorrhage have been detected using different methods [4,37,71].Most of the damage typically caused by shock-wave treatments is routinely observed and is not considered to represent severe pathological change, but the occurrence of perirenal hematoma has increased significantly in patients with pre-existing or poorly controllable hypertension [47]. Physiological tests have revealed only minor, transient reductions of renal plasma flow in the treated kidney [45,82] and no clinically relevant changes in blood chemistry [17]. Furthermore, cytoplasmic enzymes have been reported to be only transiently released into the blood and urine of shock-wave-treated patients [46]. Kishimoto et al. [46] ha...

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Effect of shock waves on suspended and immobilized L1210 cells

Cited by 79 publications

References 27 publications

Histopathology of shock wave treated tumor cell suspensions and multicell tumor spheroids

Histopathology of shock wave treated tumor cell suspensions and multicell tumor spheroids

Cavitation-generated free radicals during shock wave exposure: Investigations with cell-free solutions and suspended cells

Biological effects of shock waves

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