Effect of serum starvation on the efficiency of nuclear transfer using odd-eyed white cat fibroblasts

Yin, Xi-Jun; Lee, Hyo-Sang; Kim, Lyoung-Ho; Shin, Hyoung-Doo; Kim, Nam‐Hyung; Kong, Il‐Keun

doi:10.1016/j.theriogenology.2006.10.010

Cited by 23 publications

(19 citation statements)

References 20 publications

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“…It was of interest that the same kind of morphology has also been reported in intergeneric cloned monkey embryos when reconstructed using bovine cytoplasts [18]. Furthermore, considering the fact that Yin et al [2] succeeded in producing cloned domestic kittens by transferring the cloned domestic cat embryos with morphology and total cell numbers similar to those in our study into the oviduct of the recipients, this same in vivo environment after embryo transfer might have supported the development of embryos through implantation till the birth of kittens [2].…”

Section: Discussionmentioning

confidence: 74%

“…To date there have been several reports describing the source [3,24,25] and modification [2,6] of the donor cells and the nuclear transfer procedures [6,26] that have effects on the in vitro development of cloned domestic cat embryos. However, the effect of the holding medium used for manipulating the cat oocyte during NT procedures on subsequent in vitro development has not yet been reported.…”

Section: Discussionmentioning

confidence: 99%

“…57: [385][386][387][388][389][390][391][392] 2011) is the world's first domestic cat (DC) cloned using an adult somatic cell as a donor nucleus [1]. To date, several reports have successfully documented domestic kittens [2][3][4][5] cloned by NT. There are many efforts currently underway to study the conservation of endangered felid species.…”

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confidence: 99%

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The Effects of Manipulation Medium, Culture System and Recipient Cytoplast on In Vitro Development of Intraspecies and Intergeneric Felid Embryos

Imsoonthornruksa

Lorthongpanich

Sangmalee

et al. 2011

Journal of Reproduction and Development

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Abstract. The aim of this study was to investigate if reconstructed felid embryos obtained by intraspecies or intergeneric cloning can develop in vitro. Fibroblast cells (f) from a domestic cat (DCf), marbled cat (MCf) and bovine (Bf) were used as donor cells, and oocytes (o) from domestic cats (DCo) and bovine (Bo) were used as recipient cytoplasts. There were two intraspecies (donor cell + recipient cytoplast: DCf + DCo and Bf + Bo) and three intergeneric (MCf + DCo, DCf + Bo and MCf + Bo) cloning groups in the study. In Experiment 1, the effects of manipulation media, modified or Emcare holding medium (EHM), on in vitro development of DCf + DCo embryos were investigated. The blastocyst formation rate (BFR) of the embryos manipulated in EHM (33.3%) was higher (P<0.05) compared with those manipulated in 199H (18.1%). In Experiment 2, DCf + DCo and MCf + DCo embryos were cocultured with or without domestic cat oviductal epithelium cells. Irrespective of coculture, the same BFR was obtained for DCf + DCo embryos (44.4 vs. 38.0%), while MCf + DCo embryos could not develop beyond the morula stage. In experiment 3, although the development of MCf + DCo and DCf + Bo embryos was arrested at the morula stage, 8.6% of MCf + Bo embryos were able to develop to the blastocyst stage. These results demonstrated that EHM was superior to 199H as an embryo manipulation medium and that the DCo and Bo could support the early embryonic development of intergeneric cloned marbled cat embryos up to the morula stage. However, postimplantation development still needs to be investigated. Key words: Bovine cytoplast, Development, Felid embryos, Intergeneric cloning, Marbled cat (J. Reprod. Dev. 57: [385][386][387][388][389][390][391][392] 2011) is the world's first domestic cat (DC) cloned using an adult somatic cell as a donor nucleus [1]. To date, several reports have successfully documented domestic kittens [2][3][4][5] cloned by NT. There are many efforts currently underway to study the conservation of endangered felid species. In order to preserve and increase the numbers of endangered felid species such as the African wildcat [6,7], leopard cat [3, 8], marbled cat (MC; Pardofelis marmorata) [9] and sand cat [10], nuclear transfer (NT) has been used to produce cloned embryos.Interspecies or intergeneric NT is a high potential technique for conserving a number of endangered species. One such species is the MC, a wildcat that is considered at risk of extinction in Southeast Asia. The size of the MC is larger than a big DC, and it has a base fur color that ranges from brownish to grey with a pattern of irregular dark marbled blotches and spots outlined with black strips. The declining population could be attributed to poaching and human destruction of the natural ecosystem. Although the remaining population of the MC is not known, the International Union for the Conservation of Nature and Natural Resources (IUCN) classifies the MC as intermediate and the Convention on International Trade in Endangered Species of Wild Fauna and Flo...

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

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The Effects of Manipulation Medium, Culture System and Recipient Cytoplast on In Vitro Development of Intraspecies and Intergeneric Felid Embryos

Imsoonthornruksa

Lorthongpanich

Sangmalee

et al. 2011

Journal of Reproduction and Development

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“…Feline SCNT techniques can be applied to the conservation of endangered wild feline species [8] or the preparation of feline embryonic stem cells (ES cells) [9]. However, the current utility of SCNT in such applications is limited because the production efficiency of cloned cats is low [7,8,[10][11][12]. Thus, it is essential to establish a more efficient system to promote the use of SCNT in cats.…”

mentioning

confidence: 99%

“…However, there have been few studies investigating such factors in cats [10,11,21,22], so further research is necessary. We had previously demonstrated that cloned piglets could be efficiently obtained using preadipocytes (derived from mature adipocytes) as donor cells in porcine SCNT [23,24].…”

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confidence: 99%

In Vitro Development and Postvitrification Survival of Cloned Feline Embryos Derived from Preadipocytes

Tomii

Ogawa

Imai

et al. 2011

Journal of Reproduction and Development

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Abstract. The aim of the present study was to optimize the conditions for in vitro development and postvitrification survival of somatic cell cloned feline embryos. To determine the effects of cell cycle synchronization of the nuclear donor cells, we cultured preadipocytes under serum starvation or conventional conditions. After two days in serum starvation culture, the proportion of synchronized donor cells at the G0/G1 phase was 91.6%. This was significantly higher than the proportion of non-synchronized cells in the proliferative phase (72.6%, P<0.05). The in vitro development of somatic cell nuclear transfer (SCNT) embryos reconstructed using donor cells treated under serum starvation conditions (normal cleavage rate of 65.7%, 46/70, and blastocyst formation rate of 20.0%, 14/70) was comparable to that of the serum supplemented group (52.5%, 31/59, and 20.3%, 12/59). Use of in vitro or in vivo matured oocytes as recipient cytoplasts equally supported development of the SCNT embryos to the blastocyst stage (11.9%, 5/42, vs. 9.5%, 2/21). SCNT-derived blastocysts were vitrified using the original minimum volume cooling (MVC) or the modified (stepwise) MVC method. Although none (n=10) of the SCNT blastocysts survived following vitrification by the original MVC method, the stepwise MVC method resulted in 100% survival after rewarming (n=11).In conclusion, we demonstrated that feline somatic cell cloned embryos with a high developmental ability can be produced irrespective of cell cycle synchronization of donor cells using either in vivo or in vitro matured oocytes. Furthermore, by utilizing a stepwise vitrification method, we showed that it is possible to cryopreserve cloned feline blastocysts.

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Production of cloned cats using additional complimentary cytoplasm

Song

Lee

et al. 2019

Animal Reproduction Science

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Effect of serum starvation on the efficiency of nuclear transfer using odd-eyed white cat fibroblasts

Cited by 23 publications

References 20 publications

The Effects of Manipulation Medium, Culture System and Recipient Cytoplast on In Vitro Development of Intraspecies and Intergeneric Felid Embryos

The Effects of Manipulation Medium, Culture System and Recipient Cytoplast on In Vitro Development of Intraspecies and Intergeneric Felid Embryos

In Vitro Development and Postvitrification Survival of Cloned Feline Embryos Derived from Preadipocytes

Production of cloned cats using additional complimentary cytoplasm

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