Production of cloned cats using additional complimentary cytoplasm

Song, Seok-Hwan; Lee, Kyeong-Lim; Xu, Lianguang; Joo, Myeong-Don; Hwang, Ji-Yoon; Oh, Seon-Hwa; Kong, Il‐Keun

doi:10.1016/j.anireprosci.2019.106125

Cited by 14 publications

(19 citation statements)

References 70 publications

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“…During enucleation, some of the cytoplasm is lost, the somatic cells are reprogrammed, and embryonic genome activation is delayed. In CICT, the volume of enucleated oocytes is restored by injecting extra cytoplasm from the donor oocyte, thereby improving the efficiency of the cloned embryos [7,8]. However, the shortcomings of SCNT are also barriers to CICT.…”

Section: Discussionmentioning

confidence: 99%

“…Cytoplasm injection cloning technology (CICT) is a type of SCNT, where extra cytoplasm is injected into the enucleated oocyte with the donor cell, which improves the quality of cloned embryos [7]. Several studies have identified that CICT is more efficient than the traditional SCNT approach and has been successfully used to clone several livestock 2 of 13 animals, such as cattle, cats, and mice [7][8][9]. Several studies have focused on this new technique, which can be of great practical value.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Donor Cell Types on the Development of Bovine Embryos Using Cytoplasm Injection Cloning Technology

Song

Idrees

et al. 2021

IJMS

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Cytoplasm injection cloning technology (CICT) is an efficient technique for evaluating the developmental potential of cloned embryos. In this study, we investigated the effects of donor cell type on the developmental potential and quality of cloned bovine embryos. Adult fibroblasts (AFs) and embryonic cells (ECs) were used as donor cells to clone bovine embryos using CICT. We initially used AF cells to develop cloned embryos and then cultured the cloned day-8 blastocysts for 10 days to obtain ECs as donor cells for second embryo cloning. We found that the bovine blastocysts cloned using AF cells had significantly reduced developmental rates, embryo quality, and ratios of inner cell mass (ICM) to the total number of cells compared to those using ECs as donor cells. Furthermore, there were significant differences in the DNA methyltransferase-, histone deacetylation-, apoptosis-, and development-related genes at the blastocyst stage in embryos cloned from AFs compared to those in embryos cloned from ECs. Our results suggest that using ECs as donor cells for nuclear transfer enhances the quantity and quality of cloned embryos. However, further investigation is required in terms of determining pregnancy rates and developing cloned embryos from different donor cell types.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Donor Cell Types on the Development of Bovine Embryos Using Cytoplasm Injection Cloning Technology

Song

Idrees

et al. 2021

IJMS

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“…(3) sheep [21][22][23][24][25][26]; (4) pigs [27][28][29][30][31][32][33][34][35][36][37][38][39][40][41]; (5) equids-domestic horses [42][43][44][45][46] and mules [47]; (6) water buffaloes-Chinese swamp buffaloes [48,49] and Indian river/riverine buffaloes [50][51][52][53]; (7) one-humped or dromedary camels [7,[54][55][56]; (8) two-humped or Bactrian camels [57]; (9) domestic cats [58][59][60][61][62][63]; (10) domestic dogs…”

Section: Biotechnological Possibilities Of Applying the Techniques Ofmentioning

confidence: 99%

Extranuclear Inheritance of Mitochondrial Genome and Epigenetic Reprogrammability of Chromosomal Telomeres in Somatic Cell Cloning of Mammals

Samiec

Skrzyszowska

2021

IJMS

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The effectiveness of somatic cell nuclear transfer (SCNT) in mammals seems to be still characterized by the disappointingly low rates of cloned embryos, fetuses, and progeny generated. These rates are measured in relation to the numbers of nuclear-transferred oocytes and can vary depending on the technique applied to the reconstruction of enucleated oocytes. The SCNT efficiency is also largely affected by the capability of donor nuclei to be epigenetically reprogrammed in a cytoplasm of reconstructed oocytes. The epigenetic reprogrammability of donor nuclei in SCNT-derived embryos appears to be biased, to a great extent, by the extranuclear (cytoplasmic) inheritance of mitochondrial DNA (mtDNA) fractions originating from donor cells. A high frequency of mtDNA heteroplasmy occurrence can lead to disturbances in the intergenomic crosstalk between mitochondrial and nuclear compartments during the early embryogenesis of SCNT-derived embryos. These disturbances can give rise to incorrect and incomplete epigenetic reprogramming of donor nuclei in mammalian cloned embryos. The dwindling reprogrammability of donor nuclei in the blastomeres of SCNT-derived embryos can also be impacted by impaired epigenetic rearrangements within terminal ends of donor cell-descended chromosomes (i.e., telomeres). Therefore, dysfunctions in epigenetic reprogramming of donor nuclei can contribute to the enhanced attrition of telomeres. This accelerates the processes of epigenomic aging and replicative senescence in the cells forming various tissues and organs of cloned fetuses and progeny. For all the above-mentioned reasons, the current paper aims to overview the state of the art in not only molecular mechanisms underlying intergenomic communication between nuclear and mtDNA molecules in cloned embryos but also intrinsic determinants affecting unfaithful epigenetic reprogrammability of telomeres. The latter is related to their abrasion within somatic cell-inherited chromosomes.

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“…This protocol is conducted inside the zona pellucida, which provides protection against external environments, unlike EA and HMC. In feline species, the CICT method has been demonstrated to have enhanced embryo development and production of viable offspring over traditional cloning methods (Song et al, 2019).…”

Section: Figmentioning

confidence: 99%

“…Analysis of CICT cloned embryos shows that reprogramming of the nuclei had occurred and the embryo quality was improved, including total cell number (trophectoderm [TE]), genome methylation, and histone deacetylation, in the bovine embryo in comparison with the traditional cloning method. Moreover, CICT has been applied in cats, resulting in improved production of kittens from cloned cat embryos (Song et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Effect of Additional Cytoplasm of Cloned Embryo on In Vitro Developmental Competence and Reprogramming Efficiency in Mice

Song

et al. 2020

Cellular Reprogramming

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Somatic cell nuclear transfer (SCNT) is an important technique for biological science research. Cytoplasm injection cloning technology (CICT) was developed to improve the reprogramming efficiency as well as to overcome the limitations of SCNT. CICT uses an additional cytoplasm fused with an enucleated oocyte to restore the cytoplasmic volume of the cloned embryo, and this method could improve the reprogramming efficiency of the cloned embryo. In this study, we show that CICT can be adapted to mouse species to overcome the inefficiency of the SCNT method. In this study, results indicate that the two-cell embryo and blastocyst rates of cloned embryos with the use of the CICT method were significantly higher (p < 0.05) than that of the SCNT method (96.6%-1.1% vs. 86.7%-6.0%, 29.5%-2.6% vs. 22.1%-3.0%, respectively). Furthermore, the apoptotic cell number per blastocyst was significantly lower in the CICT group than that in the SCNT group (1.7-0.2 vs. 2.9-0.3, p < 0.05). Moreover, the acH3K9/K14 expression level in the CICT group was greater than that of the SCNT group (p < 0.05), and the relative acH3K56 level in the CICT group was significantly (p < 0.05) higher than that in the SCNT group. These results indicate that CICT helps improve the in vitro developmental competence and quality of cloned embryos.

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Production of cloned cats using additional complimentary cytoplasm

Cited by 14 publications

References 70 publications

Effects of Donor Cell Types on the Development of Bovine Embryos Using Cytoplasm Injection Cloning Technology

Effects of Donor Cell Types on the Development of Bovine Embryos Using Cytoplasm Injection Cloning Technology

Extranuclear Inheritance of Mitochondrial Genome and Epigenetic Reprogrammability of Chromosomal Telomeres in Somatic Cell Cloning of Mammals

Effect of Additional Cytoplasm of Cloned Embryo on In Vitro Developmental Competence and Reprogramming Efficiency in Mice

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