Effect of serum and surface characteristics on Candida albicans biofilm formation

Frade, João P.; Arthington-Skaggs, Beth A.

doi:10.1111/j.1439-0507.2010.01862.x

Cited by 56 publications

(38 citation statements)

References 31 publications

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“…It has been shown that the degree of biofilm formation varies and correlates with five different switch phenotypes of C. albicans (12). Several factors, including host and surface properties, artificial saliva and different environmental conditions affect biofilm formation in C. albicans (25-27). In addition, biofilm formation also differed among different species of Candida (28).…”

Section: Discussionmentioning

confidence: 99%

Biofilm Formation and Susceptibility to Amphotericin B and Fluconazole inCandida albicans

Mahmoudabadi

Zarrin

Kiasat

2014

Jundishapur J Microbiol

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Background:The ability of Candida albicans to form biofilms and adhere to host tissues and biomaterial surfaces is an important factor in its pathogenesis. One of the main characteristics of biofilms is their resistance to broad-spectrum anti-microbial drugs.Objectives:In the present study the formation of biofilm by C. albicans from different sources was evaluated. In addition, the minimum biofilm inhibitory concentration (MBIC) for two antifungals was evaluated.Materials and Methods:In total, 120 isolates of C. albicans from different sources (patients with vaginitis, patients with candiduria, bucal cavity and environmental surfaces) were collected. Biofilm formation was determined by the 96-well micro-titeration plate method. MBIC testing was also performed, using the calorimetric indicator resazurin for amphotericin B and fluconazole.Results:The results indicated that 100% of C. albicans isolates from different sources had the ability to form biofilms in vitro. Amongst these isolates, 83.3% of isolates had the maximum potential (4+) to form biofilms, while only one (0.9%) of isolates had the minimum ability (1+) to form biofilms. Our results showed that 65.0% of the tested isolates are sensitive to amphotericin B at amounts lower than 10 µg/mL, while only 26.7% are sensitive to fluconazole (had MBIC < 10 µg/mL).Conclusions:Although biofilm formation was detected in all tested isolates, there were differences in the ability to form biofilms between isolates from different sources. In addition, there were differences in the MBIC against the two examined antifungals, amphotericin B and fluconazole.

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Section: Discussionmentioning

confidence: 99%

Biofilm Formation and Susceptibility to Amphotericin B and Fluconazole inCandida albicans

Mahmoudabadi

Zarrin

Kiasat

2014

Jundishapur J Microbiol

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“…For example, it has been shown that an increase of the hydrophobicity and roughness of surface materials leads to elevated biofilm formation by Candida spp. (9,10). Adhesion molecules involved in surface adhesion have been well characterized in C. albicans.…”

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confidence: 99%

Characterization of Biofilm Formation and the Role of BCR1 in Clinical Isolates of Candida parapsilosis

et al. 2014

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eIn Candida parapsilosis, biofilm formation is considered to be a major virulence factor. Previously, we determined the ability of 33 clinical isolates causing bloodstream infection to form biofilms and identified three distinct groups of biofilm-forming strains (negative, low, and high). Here, we establish two different biofilm structures among strains forming large amounts of biofilm in which strains with complex spider-like structures formed robust biofilms on different surface materials with increased resistance to fluconazole. Surprisingly, the transcription factor Bcr1, required for biofilm formation in Candida albicans and C. parapsilosis, has an essential role only in strains with low capacity for biofilm formation. Although BCR1 leads to the formation of more and longer pseudohyphae, it was not required for initial adhesion and formation of mature biofilms in strains with a high level of biofilm formation. Furthermore, an additional phenotype affected by BCR1 was the switch in colony morphology from rough to crepe, but only in strains forming high levels of biofilm. All bcr1⌬/⌬ mutants showed increased proteolytic activity and increased susceptibility to the antimicrobial peptides protamine and RP-1 compared to corresponding wild-type and complemented strains. Taken together, our results demonstrate that biofilm formation in clinical isolates of C. parapsilosis is both dependent and independent of BCR1, but even in strains which showed a BCR1-independent biofilm phenotype, BCR1 has alternative physiological functions.

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“…C. albicans is known to interact with these ubiquitous matricellular proteins through specific interactions, which may enhance tissue invasion and contribute to virulence (43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53)(54). Given the evidence for the likely involvement of these proteins in Candida pathogenicity, pioneering biofilm investigations have emphasized the importance of a host conditioning fluid for optimal biofilm formation and the need to account for the proteins in in vitro biofilm models (29,35,(55)(56)(57)(58). However, little is known about how assimilation of host proteins may affect biofilm structure and function, perhaps even fostering biofilm development.…”

Section: Discussionmentioning

confidence: 99%

“…Numerous host factors have been shown to influence fungal biofilms (27)(28)(29)(30)(31)(32). In vitro studies have often included proteins and other factors to mimic the niche site infection (1).…”

Section: Discussionmentioning

confidence: 99%

Host Contributions to Construction of Three Device-Associated Candida albicans Biofilms

et al. 2015

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e Among the most fascinating virulence attributes of Candida is the ability to transition to a biofilm lifestyle. As a biofilm, Candida cells adhere to a surface, such as a vascular catheter, and become encased in an extracellular matrix. During this mode of growth, Candida resists the normal immune response, often causing devastating disease. Based on scanning electron microscopy images, we hypothesized that host cells and proteins become incorporated into clinical biofilms. As a means to gain an understanding of these host-biofilm interactions, we explored biofilm-associated host components by using microscopy and liquid chromatography-mass spectrometry. Here we characterize the host proteins associated with several in vivo rat Candida albicans biofilms, including those from vascular catheter, denture, and urinary catheter models as well as uninfected devices. A conserved group of 14 host proteins were found to be more abundant during infection at each of the niches. The host proteins were leukocyte and erythrocyte associated and included proteins involved in inflammation, such as C-reactive protein, myeloperoxidase, and alarmin S100-A9. A group of 59 proteins were associated with both infected and uninfected devices, and these included matricellular and inflammatory proteins. In addition, site-specific proteins were identified, such as amylase in association with the denture device. Cellular analysis revealed neutrophils as the predominant leukocytes associating with biofilms. These experiments demonstrate that host cells and proteins are key components of in vivo Candida biofilms, likely with one subset associating with the device and another being recruited by the proliferating biofilm.

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Effect of serum and surface characteristics on Candida albicans biofilm formation

Cited by 56 publications

References 31 publications

Biofilm Formation and Susceptibility to Amphotericin B and Fluconazole inCandida albicans

Biofilm Formation and Susceptibility to Amphotericin B and Fluconazole inCandida albicans

Characterization of Biofilm Formation and the Role of BCR1 in Clinical Isolates of Candida parapsilosis

Host Contributions to Construction of Three Device-Associated Candida albicans Biofilms

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