Effect of Sertoli Cell Transplantation on Reducing Neuroinflammation-Induced Necroptosis and Improving Motor Coordination in the Rat Model of Cerebellar Ataxia Induced by 3-Acetylpyridine

Saeidikhoo, Sara; Ezi, Samira; Khatmi, Aysan; Aghajanpour, Fakhroddin; Soltani, Reza; Abdollahifar, Mohammad‐Amin; Jahanian, Ali; Aliaghaei, Abbas

doi:10.1007/s12031-020-01522-x

Cited by 11 publications

(6 citation statements)

References 52 publications

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“…The suppressive effect of neurotrophic factors such as GDNF 35 , 36 , BDNF 37 , 38 , VEGF 39 , and NGF 40 on the apoptotic pathway has been reported. Similarly, studies have reported the suppression of the necroptosis pathway by GDNF, VEGF, and NGF owing to their anti-inflammatory properties 41 , 42 . We have previously reported that the expression of BDNF and NGF was significantly higher in rcMSCs than in rbMSCs 7 , 43 .…”

Section: Discussionmentioning

confidence: 91%

Transplantation of rat cranial bone-derived mesenchymal stem cells promotes functional recovery in rats with spinal cord injury

Maeda

Otsuka

Takeda

et al. 2021

Sci Rep

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Cell-based therapy using mesenchymal stem cells (MSCs) is a novel treatment strategy for spinal cord injury (SCI). MSCs can be isolated from various tissues, and their characteristics vary based on the source. However, reports demonstrating the effect of transplanted rat cranial bone-derived MSCs (rcMSCs) on rat SCI models are lacking. In this study, we determined the effect of transplanting rcMSCs in rat SCI models. MSCs were established from collected bone marrow and cranial bones. SCI rats were established using the weight-drop method and transplanted intravenously with MSCs at 24 h post SCI. The recovery of motor function and hindlimb electrophysiology was evaluated 4 weeks post transplantation. Electrophysiological recovery was evaluated by recording the transcranial electrical stimulation motor-evoked potentials. Tissue repair after SCI was assessed by calculating the cavity ratio. The expression of genes involved in the inflammatory response and cell death in the spinal cord tissue was assessed by real-time polymerase chain reaction. The transplantation of rcMSCs improved motor function and electrophysiology recovery, and reduced cavity ratio. The expression of proinflammatory cytokines was suppressed in the spinal cord tissues of the rats that received rcMSCs. These results demonstrate the efficacy of rcMSCs as cell-based therapy for SCI.

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Section: Discussionmentioning

confidence: 91%

Transplantation of rat cranial bone-derived mesenchymal stem cells promotes functional recovery in rats with spinal cord injury

Maeda

Otsuka

Takeda

et al. 2021

Sci Rep

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“…Proteolytic enzymes, which are produced and released by Sertoli cells in the testicular seminiferous tubule, play a key role in spermatogenesis [ 21 , 22 ]. Only little proteolytic activity was detected in Sertoli cell-conditioned supernatants, suggesting that the proteases under investigation were mostly found on Sertoli cell membranes [ 23 ]. The peptide hormones utilized in this research were discovered to be involved in the endocrine, paracrine, or autocrine control of testicular cells [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Whole Exome Sequencing and In Silico Analysis of Human Sertoli in Patients with Non-Obstructive Azoospermia

Azizi

Karoii

Skutella

2022

IJMS

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Non-obstructive azoospermia (NOA) is a serious cause of male infertility. The Sertoli cell responds to androgens and takes on roles supporting spermatogenesis, which may cause infertility. This work aims to enhance the genetic diagnosis of NOA via the discovery of new and hub genes implicated in human NOA and to better assess the odds of successful sperm extraction according to the individual’s genotype. Whole exome sequencing (WES) was done on three NOA patients to find key genes involved in NOA. We evaluated genome-wide transcripts (about 50,000 transcripts) by microarray between the Sertoli of non-obstructive azoospermia and normal cells. The microarray analysis of three human cases with different non-obstructive azoospermia revealed that 32 genes were upregulated, and the expressions of 113 genes were downregulated versus the normal case. For this purpose, Enrich Shiny GO, STRING, and Cytoscape online evaluations were applied to predict the functional and molecular interactions of proteins and then recognize the master pathways. The functional enrichment analysis demonstrated that the biological process (BP) terms “inositol lipid-mediated signaling”, “positive regulation of transcription by RNA polymerase II”, and “positive regulation of DNA-templated transcription” significantly changed in upregulated differentially expressed genes (DEGs). The BP investigation of downregulated DEGs highlighted “mitotic cytokinesis”, “regulation of protein-containing complex assembly”, “cytoskeleton-dependent cytokinesis”, and the “peptide metabolic process”. Overrepresented molecular function (MF) terms in upregulated DEGs included “ubiquitin-specific protease binding”, “protease binding”, “phosphatidylinositol trisphosphate phosphatase activity”, and “clathrin light chain binding”. Interestingly, the MF analysis of the downregulated DEGs revealed overexpression in “ATPase inhibitor activity”, “glutathione transferase activity”, and “ATPase regulator activity”. Our findings suggest that these genes and their interacting hub proteins could help determine the pathophysiologies of germ cell abnormalities and infertility.

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“…Due to SCs' ability to decrease necroptosis and hence extend cell longevity, their ndings predicted improved motor function and behavioral traits. Also In vitro research showed that SC-CM enhanced cell viability while lowering ROS levels signi cantly (21).…”

Section: Amyotrophic Lateral Sclerosismentioning

confidence: 98%

“…These symptoms can cause a tremor with a high amplitude that is accompanied by movement. Purkinje cell abnormalities may contribute to the pathophysiology of ataxias and disturbance in action potential production, according to ndings from cell and animal experiments (21).…”

Section: Amyotrophic Lateral Sclerosismentioning

confidence: 99%

Preclinical study of Therapeutic application of Sertoli Cells transplantation for treatment of Neurodegenerative Diseases: A Systematic Review

Ahmadi

Mehboudi

Vatandoust

et al. 2022

Preprint

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Progressive loss of neuronal function, brain atrophy, and abnormal protein deposition are the hallmarks of neurodegenerative diseases (NDs). NDs affect various parts of the brain and have various etiologies. Despite massive attempts to discover treatments for NDs during the past decades, there is still a need for effective therapeutic agents. Sertoli Cells (SCs), whether transplanted as allogeneic or xenogeneic cells, could offer a feasible opportunity for improving graft persistence by secreting immunomodulatory and trophic substances which shield replaced cells and tissues from the immune system. To date, there is no coherent information about the neuroprotective effects of SCs transplantation on NDs. Hence, this review tried to evaluate and report coherent information on the neuroprotective effects of these cells on NDs in preclinical settings. In this systematic review, the databases including, Scopus, PubMed, and ISI WEB of science were considered as a motor engine to search and get the relevant scientific articles and full text published between 2000 to 2022. Our search revealed 9 related articles on the effectiveness of SCs transplantation on NDs. Here, most of the evidence shows that SCs transplantation has promising effects on improving the symptoms of neurological diseases in rats. most of these relevant studies emphasize that many standardized preclinical trials should be conducted to provide reliable statistical evidence to confirm the application of SCs transplantation and the improvement of symptoms of neurodegenerative diseases.

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Effect of Sertoli Cell Transplantation on Reducing Neuroinflammation-Induced Necroptosis and Improving Motor Coordination in the Rat Model of Cerebellar Ataxia Induced by 3-Acetylpyridine

Cited by 11 publications

References 52 publications

Transplantation of rat cranial bone-derived mesenchymal stem cells promotes functional recovery in rats with spinal cord injury

Transplantation of rat cranial bone-derived mesenchymal stem cells promotes functional recovery in rats with spinal cord injury

Whole Exome Sequencing and In Silico Analysis of Human Sertoli in Patients with Non-Obstructive Azoospermia

Preclinical study of Therapeutic application of Sertoli Cells transplantation for treatment of Neurodegenerative Diseases: A Systematic Review

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