Effect of serotonin treatment on intestinal transport in the rabbit.

Donowitz, Mark; Charney, Alan N.; Heffernan, Joseph

doi:10.1152/ajpendo.1977.232.1.e85

Cited by 52 publications

(39 citation statements)

References 39 publications

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“…Previous studies have shown that 5-HT administration in vivo induces Cl Ϫ secretion in the rabbit small intestine (31) and acts as a mediator of diarrhea in patients with carcinoid syndrome associated with a net secretion of water and electrolytes (32). Therefore, these studies suggest an important role of 5-HT in modifying electrolyte transport process that could involve either stimulation of Cl Ϫ secretion or a decrease in Na ϩ and Cl -absorption.…”

Section: -Htmentioning

confidence: 71%

Involvement of c-Src and Protein Kinase Cδ in the Inhibition of Cl-/OH- Exchange Activity in Caco-2 Cells by Serotonin

Saksena

Gill

Tyagi

et al. 2005

Journal of Biological Chemistry

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The gastrointestinal tract is an important source of the endogenous amine, serotonin, mainly stored in the mucosal enterochromaffin cells and in the enteric nervous system (1). 5-HT1 is continuously released from the enterochromaffin cells in the intestinal mucosa into the portal circulation and the gut lumen in response to a variety of luminal stimuli such as change in pH or osmolarity and mechanical and chemical stimuli (2). 5-HT is an important neurotransmitter and intercellular messenger to modulate gastrointestinal functions (3). 5-HT has been shown to alter gastrointestinal motility (4), alter gastric acid secretion, and enhance intestinal secretions (1, 5). Most of the previous studies regarding the effects of serotonin on ion transport were mainly based on the electrophysiological data including short circuit current measurements and unidirectional fluxes. These studies showed that 5-HT stimulated electrogenic Cl Ϫ secretion and inhibited Na ϩ and Cl Ϫ absorption in the rat colon (6), jejunum (7), ileum (8), and guinea pig ileum (9). 5-HT has also been shown to inhibit the NaClabsorptive process in the rabbit ileum and gall bladder (10) but has no effect in rabbit and guinea pig colon (10, 11). Moreover, Sundaram et al. (12) have demonstrated that 5-HT inhibits Cl Ϫ /HCO 3 Ϫ exchange activity in villus cells and stimulates HCO 3 Ϫ secretion in crypt cells of the rabbit ileum. In contrast, 5-HT appears to have no significant effect on coupled NaCl absorption in the human jejunum (13).5-HT exerts its effects by binding to 5-HT receptors, which are distributed on the neuronal, muscular, and epithelial structures (14). Based on pharmacological studies and molecular cloning (15), the existence of at least seven 5-HT receptors (5-HT1-7) subdivided into 14 subtypes has been discovered. With the exception of 5-HT5 and 5-HT6, 5-HT1 A , 5-HT1 P , 5-HT2, 5-HT3, and 5-HT4 have been reported to be expressed in the gut (16). 5-HT1, 5-HT2, and 5-HT4 receptors are coupled via guanine nucleotide binding (G) proteins to their effectors, whereas 5-HT3 receptor is a ligand-gated ion channel permeable to anions and cations (17).

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Section: -Htmentioning

confidence: 71%

Involvement of c-Src and Protein Kinase Cδ in the Inhibition of Cl-/OH- Exchange Activity in Caco-2 Cells by Serotonin

Saksena

Gill

Tyagi

et al. 2005

Journal of Biological Chemistry

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“…There is already substantial evidence that 5-HT affects transepithelial ion transport in the gut of a number of species including the rat (Hardcastle et al, 1981;Zimmerman & Binder, 1984), rabbit (Donowitz et al, 1977) and guinea-pig (Cooke & Carey, 1985). Some have considered that 5-HT acts directly on the enterocytes (Zimmerman & Binder, 1984) yet radioligand binding studies failed to show binding sites on the epithelial cells although they were present in the lamina propria (Gaginella et al, 1983;Gershon et al, 1983).…”

Section: Discussionmentioning

confidence: 99%

Neuronal involvement in type 1 hypersensitivity reactions in gut epithelia

Baird¹,

Cuthbert²

1987

British J Pharmacology

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1 Using a number of 5-hydroxytryptamine (5-HT)-antagonists we have compared their activity against the chloride-secretory response in guinea-pig ileal and colonic epithelia when challenged with 5-HT or antigen. Guinea-pigs sensitized to P-lactoglobulin (PLG) were used throughout; these were obtained by providing animals with cows' milk for drinking. 2 Of methysergide, ketanserin, cyproheptadine and ICS 205-930, only the latter inhibited both the response to 5-HT and to antigen challenge. Methysergide caused a minor, significant effect on 5-HT but not on PLG responses. Ketanserin had no effect on the responses to 5-HT, but both ketanserin and cyproheptadine inhibited the challenge to PLG.3 The data are considered in relation to the current views of receptor subtypes for 5-HT. Some of the reported inhibitors may be non-specific, while we consider there is evidence to support the view that 5-HT3-receptors (neuronal receptors) are involved both in the responses to 5-HT and to antigen challenge. 4 Tetrodotoxin mimicked the effect of ICS 205-930 on both the response to 5-HT and to antigen challenge in sensitized tissues, confirming a neuronal involvement for both types of stimuli.

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“…It is also a well known fact that serotonin causes net water and electrolyte secretion in the small intestine in vivo (Donowitz et al, 1977;McFadden et al, 1986) and in vitro (Donowitz ef al., 1980a).…”

Section: Serotonin (5-hydroxytryptamine) (5 Ht)mentioning

confidence: 99%

Effect of serotonin on D-galactose transport across the rabbit jejunum

Arruebo¹,

Mesonero²,

Murillo³

et al. 1989

Reprod. Nutr. Dévelop.

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Summary ― The patterns of storage and release of serotonin found in the enterochromaffin cells of the intestinal mucosa suggest that this hormone may be an important modulator of intestinal functions. Serotonin has been shown to produce secretion of water and electrolytes in rabbit ileum, but the hormone does not appear to interact significantly with other transport processes. The aim of the present study was to determine the effect of serotonin on D-galactose absorption in rabbit jejunum. The results obtained show that serotonin (10--8 and 10--6 M) partially reduced (by 20 and 40 % respectively) D-galactose uptake across the mucosal border. This effect was concentrationdependent, and it seemed to be caused by the inhibition of Na+-dependent sugar transport. Methysergide, an antagonist of serotonin which binds with receptor 2 of serotonin, blocked the effect of serotonin. These findings suggest that serotonin may act as a regulator of sugar intestinal absorption, and that this serotonin regulation could be mediated by a direct or indirect action of the complex serotonin―receptor, which may inhibit the Na+-dependent transport system of sugars located in the brush-border membrane.serotonin -D-galactose -intestinal transport -rabbit Résumé ― Effet de la sérotonine sur le transport du D-galactose dans le jéjunum du lapin. La sérotonine est une hormone gastro-intestinale qui se trouve principalement dans les cellules entérochromaffines de la muqueuse intestinale. L'étude du stockage comme de la libération de la sérotonine dans le tractus gastro-intestinal, a suggéré que la sérotonine peut agir comme modulateur des fonctions intestinales. La sérotonine produit la sécrétion d'eau ainsi que des électrolytes dans I iléum du lapin, mais cette hormone n'a montré aucune interaction significative avec les autres processus de transport intestinal. Le but de ce travail a été de déterminer l'effet de la sérotonine sur l'absorption du D-galactose dans le jéjunum du lapin. Les résultats obtenus montrent que la sérotonine (10-e et 10-6 M) a diminué partiellement (20 et 40 % respectivement) l'entrée du D-galactose dans la muqueuse. Cet effet dépend du degré de concentration de la sérotonine et il nous semble qu'il pourrait être une inhibition du transport des sucres dépendant du Na+. La métisergide, un antagoniste de la sérotonine qui se lie au récepteur 2 de la sérotonine, a inhibé l'effet de la sérotonine. Ces résultats suggèrent que la sérotonine pourrait jouer une fonction régulatrice sur l'absorption intestinale des sucres et que cette régulation pourrait aussi être médiée par une action directe ou indirecte du complexe récepteur-sérotonine inhibé par le système de transport des sucres dépendant du Na+ situé dans la membrane de la bordure en brosse.sérotonine -D-galactose -transport intestinal -lapin

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Effect of serotonin treatment on intestinal transport in the rabbit.

Cited by 52 publications

References 39 publications

Involvement of c-Src and Protein Kinase Cδ in the Inhibition of Cl-/OH- Exchange Activity in Caco-2 Cells by Serotonin

Involvement of c-Src and Protein Kinase Cδ in the Inhibition of Cl-/OH- Exchange Activity in Caco-2 Cells by Serotonin

Neuronal involvement in type 1 hypersensitivity reactions in gut epithelia

Effect of serotonin on D-galactose transport across the rabbit jejunum

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