Effect of seminal plasma dilution or removal on spermatozoal motion characteristics of cooled stallion semen

Jasko, D.J.; Moran, Diarmuid M.; Farlin, M.E.; Squires, E.L.

doi:10.1016/0093-691x(91)90354-g

Cited by 102 publications

(65 citation statements)

References 12 publications

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“…However, a minimum SP concentration seems to be necessary for sperm survival and protection against premature capacitation during cooled storage. Five to 20% SP are beneficial for sperm motion characteristics for up to 96 h of storage and are usually recommended for semen preservation (Varner et al, 1987;Jasko et al, 1991Jasko et al, , 1992Braun et al, 1994;Schembri et al, 2002;Katila et al, 2004;Moore et al, 2005;Love et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Dose-dependent effects of homologous seminal plasma on motility and kinematic characteristics of post-thaw stallion epididymal spermatozoa

2015

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SUMMARYPreservation of epididymal spermatozoa is important to save genetic material of endangered species and breeds, or in case of unexpected injury, which will end the breeding career of valuable sires. Seminal plasma (SP) influences sperm quality in a dosedependent manner and its addition to preserved semen immediately before insemination may be beneficial for sperm fertility. Increased plasma membrane stability of epididymal spermatozoa reduces freezing injury of cells, and the addition of SP after freezing and thawing might have activating and protecting effects on spermatozoa within the female genital tract. In this study, epididymal spermatozoa were harvested by retrograde flush of the epididymal cauda immediately after routine castration and frozen. Seminal plasma was collected from other six stallions. Homologous SP (SP from the same species, but from a different animal) was added to frozen-thawed epididymal spermatozoa at concentrations of 0, 5, 20, 50 and 80% SP. Addition of SP increased sperm motility and influenced kinematic values in a dose-dependent manner (p < 0.05). Motility improved at concentrations of 20 and 50% SP, but did not further increase at 80% SP. There was no difference in sperm motility among SP from six different donor stallions regardless of the concentrations of SP (p > 0.05). Total and progressive motility of ten frozen-thawed epididymal spermatozoa samples collected from different stallions after dilution with extender and 5, 20, 50 or 80% SP differed significantly (p < 0.05). In conclusion, addition of homologous SP to frozen-thawed stallion epididymal spermatozoa immediately improved motility in a dose-dependent manner regardless of semen quality of SP donor stallions. This might positively influence fertility when SP is added before insemination. Moreover, there seems to be a threshold level of SP concentration for optimal improvement of sperm motility.

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Section: Introductionmentioning

confidence: 99%

Dose-dependent effects of homologous seminal plasma on motility and kinematic characteristics of post-thaw stallion epididymal spermatozoa

2015

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“…In situations where sperm are going to be cryopreserved or sex-sorted, sperm can remain in seminal plasma for hours or even days in diluted form. While this has not proven to be detrimental to bovine sperm (Graham, 1994;Maxwell et al 1997), for species such as porcine and equine sperm, seminal plasma has be found to be detrimental to sperm survival during cryopreservation and extended cold storage (Pursel and Johnson, 1975;Jasko et al 1991;Brinsko et al, 2000). In some species, such as sheep, seminal plasma has been found to be beneficial, and removing seminal plasma from ram sperm causes a rapid decline in sperm viability (Ashworth et al 1994).…”

Section: Discussionmentioning

confidence: 99%

Removing Seminal Plasma Improves Sex-Sorting of Bovine Sperm.

Burroughs

Graham

Lenz

et al. 2011

Biology of Reproduction

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REMOVING SEMINAL PLASMA IMPROVES SEX-SORTING OF BOVINE SPERMExperiments for this thesis evaluated how characteristics of bovine ejaculates affect efficiency of sorting X-from Y-chromosome bearing sperm by flow cytometry/cell sorting, and further, document that removal of seminal plasma improves efficacy of sexsorting bovine sperm. In Experiment I, ejaculates were collected by artificial vagina, two each from 10 bulls with an average of one hour between collections. Semen was centrifuged to separate sperm from seminal plasma, and then sperm were re-diluted to 160x10 6 sperm per ml with staining TALP (Tyrode's albumin, lactate, pyruvate) and 0, 5, 10 or 20% seminal plasma from the same ejaculate or reciprocally from first/second ejaculates. Following incubation with Hoechst 33342, sperm were sorted and analyzed with a flow cytometer/cell sorter. The % live-oriented sperm was higher for treatments with 0% seminal plasma (64.4%) than 5 (59.6%), 10 (59.0%) and 20% (57.8%) seminal plasma (p<0.01). The % live-oriented sperm was higher for second (63.0%) than first ejaculates (56.2%). Sort rate was higher for samples with 0% seminal plasma (p<0.05).The percentages of membrane-impaired sperm were lower for 0% (16.5%) than 5 (21.9%), 10 (23.6%) or 20% (23.4%) seminal plasma (p<0.003), and for second iii ejaculates (18.2%) compared to first ejaculates (25.9%). Whether seminal plasma was from first versus second ejaculates had no effect (p>0.1). Effects of seminal plasma originating from bulls different from those whose sperm were sorted was evaluated in Experiment II. Semen collection and initial analysis were performed as in Experiment I and for all successive experiments. Seminal plasma from ejaculates of 6 bulls (3 that sorted well; 3 that sorted poorly) used in Experiment I was used with sperm collected for Experiment II as well as seminal plasma from sperm donors. Sperm were stained with Hoechst 33342 as in Experiment I, but with 10% seminal plasma in staining TALP.Only seminal plasma from one bull resulted in differences for any sorting parameters; 31% of sperm were membrane-impaired with seminal plasma from this bull compared to 16-19% for seminal plasma from other bulls (p<0.05). Sort rate was decreased (3.02x10 3 sperm per sec) compared to a range of 3.64-3.97x10 3 sperm per sec with the other seminal plasmas (p<0.05). This may have been due to contamination of the original semen sample, since this effect was only seen with one of six bulls.Experiment III investigated effects of adding bovine serum albumin (BSA) during staining with Hoechst 33342 using semen from 10 bulls. BSA was added at 0, 0.3 or 0.9% to staining TALP with either 0 or 10% seminal plasma. Although BSA has been shown to be beneficial to sperm, there was a possibility that BSA would bind Hoechst 33342, thereby reducing sorting efficiency. There was no evidence of either effect; however, sperm with 0% seminal plasma had higher % live oriented cells (65%) than sperm incubated with 10% seminal plasma (61%; p<0.05). In addition, samples conta...

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“…It has been reported that the reduce of seminal plasma rates increase the motility in semen cryopreservation [13,16,17] . It has been found that seminal plasma proteins increase the resistance of spermatozoa [18] .…”

Section: Discussionmentioning

confidence: 99%

Farklı Doz ve Sıcaklıklarda Kısa Süreli Saklanan Aygır Spermasının in vitro Değerlendirilmesi

Tirpan¹,

Akçay²,

Şen³

et al. 2015

Kafkas Univ Vet Fak Derg

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This study was conducted to evaluate the effects of different temperatures and doses on stallion sperm survival after 24 h and 48 h short-term storage. Ejaculates from four warm-blooded stallions were used in this study. Ejaculates were collected by an open-ended artificial vagina which separates fractions of semen into sterile plastic cups. Only the second and third fractions (spermatozoa-rich fractions) were used to obtain spermatozoa for the experiment. Semen was diluted with INRA 96 extender to final concentrations of 50, 100, 200 and 400x10 6 sp/ml. Diluted semen were stored at different temperatures (2, 4 or 8°C) for 24-48 h. After 24 h and 48 h cooled storage, spermatozoon motility, abnormal spermatozoon ratio and membrane integrity were evaluated. According to results the highest motility values (52.5% and 55%) were determined at 50 and 100 x10 6 sp/ml concentration at 4°C after 24 h cooled storage. After 48 h of storage, the highest motility was obtained from semen stored at 8°C. Abnormal spermatozoon and membrane integrity values were not significantly different after cooled storage. Consequently, it was determined that semen with 50 and 100 million spermatozoa/ml concentration stored at 4°C for 24 h and semen with 50, 100, 200, 400 million spermatozoa/ml concentration stored at 8°C for 48 h gave better results with respect to motility than the others. Keywords: Stallion semen, Doses, Temperature, Short-term preservation, In vitro evaluation of semen Farklı Doz ve Sıcaklıklarda Kısa Süreli Saklanan Aygır Spermasının in vitro Değerlendirilmesi ÖzetBu araştırma, farklı doz ve sıcaklıkların (24-48 saat) aygır spermatozoonlarının yaşam kabiliyetleri üzerine etkisini ortaya koymak amaçlanmıştır. Çalışmada dört sıcakkanlı aygırdan alınan ejekülatlar kullanılmıştır. Ejekülatların alınması için açık uçlu suni vagina kullanılmış ve bu yolla sperma fraksiyonlar halinde cam kaplara alınmıştır. Deney süresince sadece 2. ve 3. fraksiyonlar (spermatozoondan zengin fraksiyonlar) çalışmada kullanılmıştır. Sperma INRA 96 sulandırıcısı ile final yoğunluk 50, 100, 200, 400x10 6 spermatozoa/ ml (sp/ml) olacak şekilde sulandırılmıştır. Sulandırılan sperma 2°C, 4°C ve 8°C lik sıcaklıklarda 24-48 saat süreler ile saklanmıştır. 24-48 saatlik saklama sonrası spermatozoon motilitesi, anormal spermatozoa ve membran bütünlüğü yönünden muayene edilmiştir. Elde edilen sonuçlara göre kısa süreli saklanan spermada 24. saat sonunda en yüksek motilite değerleri (%52.5 ve %55) 4°C'de 50 ve 100 milyon yoğunlukta elde edilmiştir, 48. saat sonunda ise en yüksek motilite değerleri 8°C'de saklanan spermadan elde edilmiştir. Anormal spermatozoa ve membran bütünlüğü değerleri farklılık göstermemiştir. Sonuç olarak, 4°C'de 50 ve 100 milyon dozlarında 24 saat saklamanın ve 8°C'de 50, 100, 200 ve 400 milyon dozlarında 48 saat saklamanın diğerlerine göre daha iyi sonuçlar verdiği tespit edilmiştir.

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Effect of seminal plasma dilution or removal on spermatozoal motion characteristics of cooled stallion semen

Cited by 102 publications

References 12 publications

Dose-dependent effects of homologous seminal plasma on motility and kinematic characteristics of post-thaw stallion epididymal spermatozoa

Dose-dependent effects of homologous seminal plasma on motility and kinematic characteristics of post-thaw stallion epididymal spermatozoa

Removing Seminal Plasma Improves Sex-Sorting of Bovine Sperm.

Farklı Doz ve Sıcaklıklarda Kısa Süreli Saklanan Aygır Spermasının in vitro Değerlendirilmesi

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