Effect of seminal leukocytes on in vitro fertilization and intracytoplasmic sperm injection outcomes

Ricci, Giuseppe; Granzotto, Marilena; Luppi, Stefania; Giolo, Elena; Martinelli, Monica; Zito, Gabriella; Borelli, Massimo

doi:10.1016/j.fertnstert.2015.04.007

Cited by 35 publications

(25 citation statements)

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“…All IVF subjects were treated according to standard clinical and laboratory protocols as previously described . The patients received a long gonadotrophin‐releasing hormone (GnRH) analog protocol or a flexible GnRH antagonist protocol with oral contraceptive pre‐treatment.…”

Section: Methodsmentioning

confidence: 99%

Cervico‐vaginal secretion cytokine profile: A non‐invasive approach to study the endometrial receptivity in IVF cycles

Zanotta

Monasta

Skerk

et al. 2018

American J Rep Immunol

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Several assisted reproductive techniques (ART), including conventional in vitro fertilization (IVF) or intra-cytoplasmic sperm injection (ICSI), are currently available for treatment of infertility. In most ART treatment cycles, one or more good-quality embryos are obtained.Nevertheless, in the last world report, the mean delivery rate per aspiration for fresh cycles reached approximately the 20%, 1 suggesting that a large percentage of good-quality embryos fail to implant.Successful embryo implantation and pregnancy require not only a good-quality embryo, but also a receptive endometrium and effective endometrial-embryonic interactions. 2 This embryo-uterine cross-talk seems to be regulated by several molecules leading to modulation of different cellular functions, such as maternal steroid Problem: Cytokines have a significant role in the process of embryo implantation, trophoblast growth, and differentiation by modulating the immune and endocrine system. The aim of this study was to investigate the profile of a large set of cytokines in the cervico-vaginal washing of women undergoing IVF, to explore the association of these proteins with a good receptive endometrium. Method of study:A cohort of 155 women scheduled for IVF cycle was recruited. All patients were asymptomatic for genitourinary infections and had been screened for chlamydia, mycoplasma, and other bacterial infections. All IVF subjects were treated according to standard clinical and laboratory protocols. A panel of 48 immune factors was analyzed on cervico-vaginal washing, using magnetic bead-based multiplex immunoassays (Bio-Plex, BIO-RAD Laboratories, Milano, Italy). Results:A total of 99 patients reached embryo transfer, of which 31 had a clinical pregnancy. A pattern of four pro-inflammatory immune molecules, IL-12p40, IFN-a, MIF, and MCP3 (P < 0.001), was found significantly up-regulated in the cervico-vaginal fluid of women with clinical pregnancy. A significantly increased expression of IL-9, Gro α , and SDF-1 α (P < 0.05) was observed in the presence of endometriosis, while high levels of IL-13 and L-15 were associated with ovulatory infertility factor (P < 0.05). Conclusion:In this pilot study, we demonstrated that the expression of specific cytokines in the cervico-vaginal washing on the day of oocyte retrieval might have a positive correlation with the potential clinical pregnancy. Therefore, cervico-vaginal secretion cytokine profiling might be a new, non-invasive approach to study the endometrial receptivity in IVF management. K E Y W O R D S cytokines, endometrium, implantation, infertility, IVF, pregnancy How to cite this article: Zanotta N, Monasta L, Skerk K, et al. Cervico-vaginal secretion cytokine profile: A non-invasive approach to study the endometrial receptivity in IVF cycles. Am J Reprod Immunol. 2019;81:e13064. https://doi.

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Section: Methodsmentioning

confidence: 99%

Cervico‐vaginal secretion cytokine profile: A non‐invasive approach to study the endometrial receptivity in IVF cycles

Zanotta

Monasta

Skerk

et al. 2018

American J Rep Immunol

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“…The concentration of different subset of seminal leukocytes was not associated to the rate of conception in vivo (Tomlinson et al, 1993), or to the IVF (Tomlinson et al, 1992a) or ICSI outcome (Ricci et al, 2015). The concentration of leukocytes expressing CD45 was, however, negatively correlated with Figure 3 Flow cytometric assessment of sperm 8-hydroxy-2 0 -deoxyguanosine.…”

Section: (A) (B) (C) (D)mentioning

confidence: 99%

Semen leukocytes and oxidative-dependent DNA damage of spermatozoa in male partners of subfertile couples with no symptoms of genital tract infection

et al. 2016

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SUMMARYThe influence of seminal leukocytes on generation of oxidative damage to sperm DNA was here investigated on male partners of subfertile couples asymptomatic for a genital tract infection. The study included 111 ejaculates from men attending the Andrology Centre at University of L'Aquila. Semen leukocytes subset included round cells expressing pan-leukocyte CD45 antigen, monocyte/ macrophage lineage antigen CD14, and activated macrophages HLA-DR antigen. In conclusion, oxidative-dependent DNA damage in spermatozoa was associated to poor semen quality but not to different leukocyte subpopulations in ejaculates of men asymptomatic for a genital tract infection.

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“…In this study, independent variables such as female age, male age and semen parameters based on WHO criteria only showed significant statistical relation between percent agglutination time of semenwith pregnancy.In this research, with increasing leukocytospermic severity, the success rate of IVF was decreased, but the difference was not statistically significant, which is consistent with the results of studies. [45][46][47][48][49][50][51][52] In another study by Hasani et al, the effect of leukocytospermia in infertile men on sperm parameters was investigated. The results showed that the sperm motility in the leukocytospermic group had a significant difference between the non-leukocytospermic group and the healthy subjects.…”

Section: Discussionmentioning

confidence: 99%

“…37 A number of studies have also shown that leukocytospermia has a negative effect on the efficiency of IVF and ICSI, [38][39][40][41][42][43][44] but some studies have also shown that leukocytospermia has no effect on the incidence of pregnancy and fertility after IVF and ICSI. [45][46][47][48][49][50][51][52] Considering the different results obtained from the studies and in view of the prevalence of infertility and the methods of assisted reproduction available and also the failure to carry out a similar study in this regard, this study was conducted to determine the relationship between semen parameters and success rates IVF.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Semen Parameters on the Outcome of Induced in Vitro Fertilization (Ivf) in Infertile Couples

Alemi

Omrani

Tarhani

et al. 2019

Int J Life Sci Pharm Res

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Infertility is one of the most important crises in the lifestyle, leading to many problems, for couples. Infertility may be associated with many factors. The aim of this study is to determine the effect of semen parameters on the outcome of Induced in-Vitro Fertilization in infertile women. This study was performed as a retrospective cohort study using infertile couple's data that was provided by IVF in Hamadan University of Medical Sciences Endometrial and Endometriosis Research Center in 2016. Couples who had a normal semen parameter were compared with couples who had an abnormal semen parameter in terms of the outcome of the intervention, which included the positive increase of the β-HCG titer in two time measurements.The total number of subjects studied was 289, with mean age 30.49 years. The percentage of positive β-HCG (31.21%), incidence of fertilization (30.54%) and pregnancy (29.86%). The frequency of abnormal semen parameters based on WHO criteria in term of mobility was 53.98%, sperm number 32.22%, volume 4.45% agglutination 4.53% and leukocytospermia 12% respectively. There were a significant relationship between morphology>20% (p=0.022) and normal sperm adhesion (p=0.041) with pregnancy incidence. The viscosity and morphology of sperm, are two semen parameters that effects on the success rate of fertility Induced in-Vitro Fertilization method.

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Effect of seminal leukocytes on in vitro fertilization and intracytoplasmic sperm injection outcomes

Abstract: Using a flow cytometry method, we demonstrated that leukocytospermia does not significantly influence IVF or ICSI outcomes. The same results were obtained by using lower or higher cut-off values for leukocytospermia (from 0.2 to 2 × 10(6)/mL).

Cited by 35 publications

References 40 publications

Cervico‐vaginal secretion cytokine profile: A non‐invasive approach to study the endometrial receptivity in IVF cycles

Cervico‐vaginal secretion cytokine profile: A non‐invasive approach to study the endometrial receptivity in IVF cycles

Semen leukocytes and oxidative-dependent DNA damage of spermatozoa in male partners of subfertile couples with no symptoms of genital tract infection

The Effect of Semen Parameters on the Outcome of Induced in Vitro Fertilization (Ivf) in Infertile Couples

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