Effect of semen extenders on the motility and viability of stored African Catfish (Clarias gariepinus) spermatozoa

Adeyemo, Olanike K.; Adeyemo, O O.; Oyeyemi, M. O.; Agbede, S. A.

doi:10.4314/jasem.v11i1.46827

Cited by 11 publications

(12 citation statements)

References 15 publications

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“…Previous reports also corroborate the line of thought of this study; improved survival rate of spermatozoa preserved with tomato juice at 5°C was observed in African catfish (Clarias gariepinus; Adeyemo et al 2007). In addition, Al-Daraji (2012) reported the protective effect of orange juice on spermatozoa against the harmful effects of lipid peroxidation in white layer cocks' semen.…”

Section: Quality and Fertility Of Rabbit Semen Diluted With Watermelosupporting

confidence: 90%

Calidad y fertilidad del semen de conejo diluido con jugo de sandía.

Jimoh

Ayedun

2020

ARCH ZOOTEC

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Se diseñó un estudio para evaluar la idoneidad del jugo de sandía como diluyente del semen de conejo y la tasa de concepción conejos hembra inseminados. La sandía madura (Citrus lanatus) se obtuvo y procesó en jugo usando el procedimiento estándar. Para el ensayo se usaron un total de 10 conejo macho y 90 conejo hembra , todos de raza New Zealand White x Chinchilla. El semen se recolectó de los conejo macho utilizando una vagina artificial y se diluyó con jugo de sandía a una proporción 1: 0, 1: 0.25, 1: 0.67, 1: 1.5, 1: 4 o con solución salina normal 1:1; y se designó como T1, T2, T3, T4, T5 y T6 respectivamente. El análisis del semen se realizó en semen fresco y los diversos semen se diluyeron inmediatamente hasta 370C. Quince de cada uno fueron asignar al azar para el tratamiento diferente en un diseño completamente al azar y se inseminaron con su tratamiento respectivo utilizando el procedimiento estándar. La tasa de concepción, el tamaño de la camada y el índice de productividad al nacer se juzgado al final del período de gestación. El resultado obtenido reveló que el rango de valores obtenidos para la movilidad del espermatozoide, la integridad de la membrana estructural y la integridad del acrosoma está dentro de los valores aceptados para el semen de buena calidad. La tasa de concepción de inseminación con semen diluido con jugo de sandía a una proporción 1: 1.5 fue la tasa de dilución óptima con 75%. El tamaño de la camada fue significativamente (p

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Section: Quality and Fertility Of Rabbit Semen Diluted With Watermelosupporting

confidence: 90%

Calidad y fertilidad del semen de conejo diluido con jugo de sandía.

Jimoh

Ayedun

2020

ARCH ZOOTEC

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“…This value is in agreement with the suggested pre-extension motility of 80% and above (Akay et al, 1995;Urbanyi et al, 1999;Adeyemo et al, 2007). The poor result obtained in the test group following storage can be attributed to some factors one of which could be the repeated effect of freezing and thawing.…”

Section: Discussionsupporting

confidence: 89%

“…It is well known that Na citrate has the ability to preserve milt of Clarias gariepinus when refrigerated (Mansour et al, 2005;Adeyemo et al, 2007), thus it served as the control in this research. Results obtained showed that Na citrate at 4°C gave 20% motility and viability by day 7 post storage.…”

Section: Discussionmentioning

confidence: 99%

Effect of Sperm Sac (Testis) in Cryopreservation Protocol of Milt (Spermatozoa) of <i>Clarias Gariepinus</i>

Ubah

Okere²,

Nwankwo³

et al. 2017

ija

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This study was carried out to assess the adequacy of cryopreservation protocol of milt (spermatozoa) of Clarias garipenus, using the sperm sac (testis) intact with the objectives of evaluating the milt for percentage live, motility and mass activity for a period of seven days at -20°C. Semen samples (milt) were collected from mature male broodstocks of Clarias gariepinus of 14 months old and weighing 1.5 kg. There were two groups comprising test and control groups. Test group comprising of the sperm sac was dipped in Na-Citrate and placed in a properly labelled container and preserved at -20°C while control group comprised of milt extended in Na-Citrate dispensed into seven insulin syringes, properly labelled and stored at 4°C. These were observed for a period of seven days and percentage motility, percentage live as well as mass activity of spermatozoa of these samples were recorded. Student's t test was used in analyzing the data. Results showed statistically significant difference (p<0.05) in motility, percentage live and mass activity between test and control groups, with test group showing mean percentage motility, percentage live and mass activity of 24.29±76%, 24.29±76% and 1.14±1.8 respectively while the control showed values of 48.57±65%, 48.57± 65% and 2.0±1.0 respectively. It was concluded that use of the sperm sac at -20°C can only be adopted within three days of collection from the fish. It was recommended that farmers adopt the protocol only for a short term period to eliminate the frustration of artificial spawning failure due lack of good semen to complete the synchronous reproductive process.Keywords Sperm sac; Milt; Cryopreservation; Catfish; Artifical spawning BackgroundThe African catfish, Clarias gariepinus (Burchell, 1822) is considered one of best suitable alternatives to tilapia for subsistence for fish farming. African Catfish is a good source of animal protein and has a very tasty delicacy. It is cultured for its high growth rate, disease resistance and its' tolerant of a wide range of temperature, as well as oxygen and high salinity level (Haylor, 1993). This has attracted a lot of stakeholders whose major problem is to satisfy the increasing demand for the fish; one of the challenges of African catfish farming is making fingerlings available all year round. There is need to research on various methods of milt preservation so as to ensure the possibility of developing effective and easier methods of milt preservation. Proper preservation of milt will ensure an all year round availability of gametes to farmers and easy access to genetically improved milt for African catfish reproduction.This research work is concentrating solely on a method of preservation and it is believed with no doubt that it will give adequate information on the role of the sperm sac on preserved milt of the African catfish. This research will give more insight on the adequacy of cryopreservation protocol of milt (spermatozoa) of Clarias gariepinus using the sperm sac (testes) intact with the objectives of ...

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“…The fruit-juice was prepared according to the procedure by Adeyemo et al (2007) with some modifications as follows: fresh cucumber, pineapple and orange were washed thoroughly using distilled water. The fruits were first peeled (pineapple and orange) and thereafter cut into pieces and the seeds were removed (cucumber and orange) and then the fruit were blended for five minutes, placed in a sieve and pressure was applied manually to squeeze the juice out from the blended fruits.…”

Section: Juice Preparationmentioning

confidence: 99%

“…Cucumber (Cucumis sativus), pineapple (Ananas comosus) and orange (Citrus sinensis) are fruit-rich natural antioxidants renowned for their high concentrations of these vitamins and other antioxidants (Cutler et al, 2008). Improved survival rate of spermatozoa preserved with tomato juice at 5°C was observed in African catfish (Clarias gariepinus; Adeyemo et al, 2007). In addition, Al-Daraji (2012) reported the protective effect of orange juice on spermatozoa against the harmful effects of lipid peroxidation of white layer cocks' semen stored for up to 72 h. Daramola and Adekunle (2015) recently observed improved progressive motility, acrosome and membrane integrities, reduced abnormalities and MDA following supplementation of extenders with pineapple and cucumber juices during refrigeration of semen obtained from WAD goat bucks.…”

Section: Introductionmentioning

confidence: 99%

Protective effects of fruit-juices on sperm viability of West African Dwarf goat bucks during cryopreservation

et al. 2016

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The present study was conducted to determine the effects of adding fruit-juices to semen extenders on viability of buck spermatozoa during cryopreservation in two studies. Pooled semen samples obtained from West African Dwarf (WAD) goat bucks were diluted with Tris-egg yolk based extenders. In study I, the diluted semen samples were supplemented with orange (Citrus sinensis), cucumber (Cucumis sativus) and pineapple (Ananas comosus) juices (2.5, 5, 7.5 and 10 ml/100 ml). In study II, the diluted semen samples were supplemented with 5 ml/100 ml of different combinations of fruitjuices consisting of pineapple and orange juices (TEYPO) at a 1:1 ratio, pineapple and cucumber juices (TEYPC) at a 1:1 ratio, orange and cucumber juices (TEYOC) at a 1:1 ratio, and pineapple, orange and cucumber juices (TEYPOC) at a 1:1:1 ratio, respectively. The diluted semen samples were cryopreserved and thereafter evaluated for sperm viability parameters. The extenders supplemented with 7.5 and 10% orange, and 10% pineapple had higher (P < 0.05) motility compared to the control. There was higher (P < 0.05) acrosome integrity in extenders supplemented with orange and pineapple at all levels compared to the control while cucumber at 10% had higher (P < 0.05) acrosome integrity compared to other levels and the control. The extenders supplemented with cucumber, orange and pineapple had higher (P < 0.05) membrane integrity compared to the control except at 7.5% of extender supplemented with cucumber. Similarly, the extenders supplemented with cucumber, orange and pineapple had lower (P < 0.05) sperm abnormality compared to the control. The extenders supplemented with cucumber, orange and pineapple at 2.5, 10 and 7.5% respectively had lower (P < 0.05) concentrations of MDA compared to other levels and the control. There was higher motility and acrosome integrity in extenders supplemented with different combinations of fruit-juices (P < 0.05) compared to the control. TEYPC had higher (P < 0.05) membrane integrity and lower (P < 0.05) concentrations of MDA compared to the control. The findings revealed that extenders supplemented with orange and pineapple at 10% consistently improved motility, acrosome integrity and membrane integrity, and reduced sperm abnormality compared to the control while no particular combination of these fruit-juices was consistently superior in all the parameters.

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Effect of semen extenders on the motility and viability of stored African Catfish (Clarias gariepinus) spermatozoa

Cited by 11 publications

References 15 publications

Calidad y fertilidad del semen de conejo diluido con jugo de sandía.

Calidad y fertilidad del semen de conejo diluido con jugo de sandía.

Effect of Sperm Sac (Testis) in Cryopreservation Protocol of Milt (Spermatozoa) of <i>Clarias Gariepinus</i>

Protective effects of fruit-juices on sperm viability of West African Dwarf goat bucks during cryopreservation

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