Effect of selenium on freezing-thawing damage of mice spermatogonial stem cell: a model to preserve fertility in childhood cancers

Boroujeni, Mandana Beigi; Peidayesh, Fatemeh; Pirnia, Afshin; Boroujeni, Nasim Beigi; Ahmadi, Seyyed Amir Yasin; Gholami, Mohammadreza

doi:10.21037/sci.2019.10.01

Cited by 8 publications

(4 citation statements)

References 22 publications

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“…Infertility occurs in almost one-third of the young boy's cancer survivors due to toxic effects of chemotherapy and radiotherapy drugs [15]. Freezing and long-term preservation of SSCs as an effective method for restoration of fertility is one of the most important subjects in clinical and research field [23,24]. Previous studies showed that normal function of SSCs decreases after the freeze-thaw process due to cryoinjuries.…”

Section: Discussionmentioning

confidence: 99%

Improving of Cryopreservation Quality of Spermatogonial Stem Cells after Freezing-Thawing by Melatonin

Farokhi

2021

OAJBS

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Cryopreservation of spermatogonial stem cells (SSCs) is an advantageous method to restore fertility in young boys' cancer survivors. Recently, for reduction of SSCs cryoinjering various antioxidants have been used. The purpose of this study was to evaluate antioxidant effects of melatonin in frozen-thawed SSCs. Spermatogonial stem cells were isolated from testes of neonate mice (3-6 days old) and their purities were measured by flow cytometry with GFRα1. After culturing, the cells were frozen in two groups 1control and 2-melatonin (100μM) for one month. Finally, the cell viability, colonization rate, intracellular ROS were evaluated after freezing thawing. Melatonin increased the viability, colonization of SSCs, and decreased intracellular ROS. The results of this study show that melatonin with antioxidant effects as a good option can be used for freezing and long-term storage of cells and infertility treatment in the clinic.

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Section: Discussionmentioning

confidence: 99%

Improving of Cryopreservation Quality of Spermatogonial Stem Cells after Freezing-Thawing by Melatonin

Farokhi

2021

OAJBS

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“…Oeda et al [80] reported that incubating human semen with 1 mg/mL NAC for https://doi.org/10.5653/cerm.2023.06016 20 minutes at room temperature significantly lowered ROS levels and improved sperm motility. Boroujeni et al [81] found that rinsing with 5 μg/mL selenium after thawing can enhance human sperm parameters and survival. Fontoura et al [82] found that adding leptin, a hormone that regulates food intake behavior, to human sperm prior to freezing supports antioxidant enzyme activity and provides additional antioxidant protection.…”

Section: Pre-freeze or Post-thaw Carementioning

confidence: 99%

New strategies for germ cell cryopreservation: Cryoinjury modulation

Jung,

Ryu

2023

Clin Exp Reprod Med

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Cryopreservation is an option for the preservation of pre- or post-pubertal female or male fertility. This technique not only is beneficial for human clinical applications, but also plays a crucial role in the breeding of livestock and endangered species. Unfortunately, frozen germ cells, including oocytes, sperm, embryos, and spermatogonial stem cells, are subject to cryoinjury. As a result, various cryoprotective agents and freezing techniques have been developed to mitigate this damage. Despite extensive research aimed at reducing apoptotic cell death during freezing, a low survival rate and impaired cell function are still observed after freeze-thawing. In recent decades, several cell death pathways other than apoptosis have been identified. However, the relationship between these pathways and cryoinjury is not yet fully understood, although necroptosis and autophagy appear to be linked to cryoinjury. Therefore, gaining a deeper understanding of the molecular mechanisms of cryoinjury could aid in the development of new strategies to enhance the effectiveness of the freezing of reproductive tissues. In this review, we focus on the pathways through which cryoinjury leads to cell death and propose novel approaches to enhance freezing efficacy based on signaling molecules.

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“…Both pathways terminates to caspase proteins, and the pathways become common from the level of caspase3 (3). Among the apoptosis proteins, Bcl2 (as a member of Bcl2 family) is an inhibitory protein while Fas, Bax and P53 are activating proteins (4).…”

Section: Introductionmentioning

confidence: 99%

A pilot design to propose an apoptosis definition based on gene expression data

Boroujeni

2022

J Parathyr Dis

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Introduction: Apoptosis is a programmed cell death commonly investigated in researches. Objectives: According to the lack of a gold standard for definition of apoptosis, we conducted a pilot analysis to propose a new definition for apoptosis based on a previous gene expression data. Materials and Methods: As a secondary analysis, a gene expression data of a vitrification thawing induced model of apoptosis conducted on ten mice ovaries was used. Half of the samples had been treated with selenium. P53, Fas, Bax and Bcl2 were considered as apoptosis related genes. Their +∆CTs were reported. An apoptosis scoring system was designed based on regression analysis. Results: In multiple regression of the genes, the only significant association was for Bcl2 expression for prediction of apoptosis. Then a model was designed consisting of Bcl2 and some interactions that the calculated amount of its formula was considered as the scoring system (R2 = 0.989, P (>F) <0.001, Root mean square deviation = 0.082). Bax/Bcl2 ratio showed an acceptable goodness of fit for prediction of this score (R2 = 0.845, P (>F) <0.001, root mean square deviation = 0.219). No conclusive result was found for factor analysis. Conclusion: The present study used a simple approach to propose statistical models for apoptosis. A comprehensive criterion should be designed apoptosis and other biological systems to be considered as a gold standard.

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Effect of selenium on freezing-thawing damage of mice spermatogonial stem cell: a model to preserve fertility in childhood cancers

Cited by 8 publications

References 22 publications

Improving of Cryopreservation Quality of Spermatogonial Stem Cells after Freezing-Thawing by Melatonin

Improving of Cryopreservation Quality of Spermatogonial Stem Cells after Freezing-Thawing by Melatonin

New strategies for germ cell cryopreservation: Cryoinjury modulation

A pilot design to propose an apoptosis definition based on gene expression data

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