Abstract:Cryopreservation of spermatogonial stem cells (SSCs) is an advantageous method to restore fertility in young boys' cancer survivors. Recently, for reduction of SSCs cryoinjering various antioxidants have been used. The purpose of this study was to evaluate antioxidant effects of melatonin in frozen-thawed SSCs. Spermatogonial stem cells were isolated from testes of neonate mice (3-6 days old) and their purities were measured by flow cytometry with GFRα1. After culturing, the cells were frozen in two groups 1co… Show more
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