Effect of selection agents to Chrysanthemum (<i>Chrysanthemum morifolium</i>) callus growth after <i>Agrobacterium</i>-mediated genetic transformation

Sjahril, Rinaldi; Jamaluddin, I; Nadir, Marhamah; Asman, Asman; Dungga, Novaty Eny

doi:10.1088/1755-1315/157/1/012044

Cited by 5 publications

(8 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nakano (2007) also reported that the optimal concentration of acetosyringone, used for enhancing Agrobacterium infection, depends on plant species, source of explants, duration of cultivation, and competency of the target tissue. This report substantiate previous study by Jamaluddin (2018) where their results showed that the efficiency of genetic transformation on callus occurred in internode (15.2%), lateral shoot buds (57.5%), and leaves (20.7%). The value of transformation efficiency (highest to lowest) on shoots regeneration was lateral shoot buds (50.0%), leaves (1.4%), and internode (0.3%).…”

Section: Introductionsupporting

confidence: 92%

“…Transformation was done by co-cultivation of leaf pieces, internodes, and lateral shoot buds in overnight-cultured Agrobacterium solution, that had been diluted to 10% (vol/vol) with liquid MS medium, for 30 minutes (Sjahril et al 2018). The explants were then filtered with nylon mesh (42 µm) and rinsed with sugar-free liquid MS medium, dried on sterile tissue paper and placed on the surface of the solid MS medium added with 200 µM acetosyringone for co-cultivation in culture bottles for 3 days in dark conditions at 25°C.…”

Section: Transformation and Transgenic Plant Regenerationmentioning

confidence: 99%

“…5, September 2022 for 10 minutes and subsequently the explants were inoculated onto the fresh medium containing 5 mg L -1 Meropenem ® . The selection agents used were kanamycin (50 mg L -1 ) for callus selection and during plantlet stage according to the results obtained in the previous study Sjahril et al 2018). Cultures were incubated under TL-28W (Himawari ® ) lighting at 25°C.…”

Section: Transformation and Transgenic Plant Regenerationmentioning

confidence: 99%

“…This is likely caused by habituation of the callus due to repeated sub-cultures and the use of growth regulators and antibiotics for long term. Other factors that are thought to affect plant regeneration are the difference in explant sources such as leaves, lateral shoot buds and internodes as was reported by Jamaluddin et al (2018). The success of the transformation is also influenced by age of explants, different ratios and concentrations of growth regulators and chemicals/antibiotics used as selective agents of transformed tissues (Bangash et al 2013).…”

Section: Introductionmentioning

confidence: 98%

See 3 more Smart Citations

Performance of Transgenic Chrysanthemum Harbouring Wasabi Defensin Gene for White Rust Disease Resistance

et al. 2022

View full text Add to dashboard Cite

This study was intended to obtain white rust (Puccinia horiana) disease resistance Chrysanthemum transformed with wasabi defensin gene through mediation of Agrobacterium tumefaciens from three explant sources, i.e., leaf, lateral shoot bud, and internode. Observations were made on transformation efficiency, PCR analysis, in vitro and ex vitro disease resistance tests. Results showed that efficiency of transgenic callus and shoot regeneration was found both highest from lateral shoot buds (57.5% and 50.0%, respectively). PCR analysis showed that three putative transgenic plantlets from lateral shoot buds and one from leaf explant were putative transgenic carrying the wasabi, hpt, and nptII genes. Rooting test showed that the highest number of rooted plants was found in treatment of hygromycin (Hg) 25 mg L-1 (81%) and lowest was in treatment combination of kanamycin (Km) 50 mg L-1 + Hg 25 mg L-1 (25%). In vitro disease resistance test with sorus inoculation of P. horiana, directly on the leaves, resulted in 20 resistant plants out of 30 putative transgenic plants (66.67%). Ex vitro testing on adult plants of the same samples in a confined closed greenhouse (CGH) resulted in average of 80% transgenic Chrysanthemum plants were resistant, whereas in control plants caused white rust disease symptom.

show abstract

Section: Introductionsupporting

confidence: 92%

Section: Transformation and Transgenic Plant Regenerationmentioning

confidence: 99%

Section: Transformation and Transgenic Plant Regenerationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Performance of Transgenic Chrysanthemum Harbouring Wasabi Defensin Gene for White Rust Disease Resistance

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Among them, Agrobacterium-mediated transformation has a unique significance and is being used for the insertion of specific genes into numerous ornamental plant species because of its authenticity and reliability. Several studies have been reported concerning the evaluation of Agrobacterium-mediated transformation in chrysanthemum [6][7][8]. The susceptibility of chrysanthemum to Agrobacterium-mediated transformation is well established and has been characterized by low transformation efficiency since 1975 [9,10].…”

Section: Introductionmentioning

confidence: 99%

Standardized Genetic Transformation Protocol for Chrysanthemum cv. ‘Jinba’ with TERMINAL FLOWER 1 Homolog CmTFL1a

Haider

Gao

2020

Genes

View full text Add to dashboard Cite

Chrysanthemum (Chrysanthemum x morifolium Ramat.) cultivar Jinba is a distinctive short-day chrysanthemum that can be exploited as a model organism for studying the molecular mechanism of flowering. The commercial value of Jinba can be increased in global flower markets by developing its proper regeneration and genetic transformation system. By addressing typical problems associated with Agrobacterium-mediated transformation in chrysanthemum, that is, low transformation efficiency and high cultivar specificity, we designed an efficient, stable transformation system. Here, we identify the features that significantly affect the genetic transformation of Jinba and standardize its transformation protocol by using CmTFL1a as a transgene. The appropriate concentrations of various antibiotics (kanamycin, meropenem and carbenicillin) and growth regulators (6-BA, 2,4-D and NAA) for the genetic transformation were determined to check their effects on in vitro plant regeneration from leaf segments of Jinba; thus, the transformation protocol was standardized through Agrobacterium tumefaciens (EHA105). In addition, the presence of the transgene and its stable expression in CmTFL1a transgenic plants were confirmed by polymerase chain reaction (PCR) analysis. The CmTFL1a transgene constitutively expressed in the transgenic plants was highly expressed in shoot apices as compared to stem and leaves. Overexpression of CmTFL1a led to a delay in transition to the reproductive phase and significantly affected plant morphology. This study will help to understand the biological phenomenon of TFL1 homolog in chrysanthemum. Moreover, our findings can explore innovative possibilities for genetic engineering and breeding of other chrysanthemum cultivars.

show abstract

Breeding of the transgenic chrysanthemum (Chrysanthemum morifolium Ramat.) carrying aphid-resistance gene, Pinellia ternata agglutinin (PTA)

Pak¹,

Han²,

Li³

et al. 2020

Plant Biotechnol Rep

View full text Add to dashboard Cite

Effect of selection agents to Chrysanthemum (Chrysanthemum morifolium) callus growth after Agrobacterium-mediated genetic transformation

Cited by 5 publications

References 11 publications

Performance of Transgenic Chrysanthemum Harbouring Wasabi Defensin Gene for White Rust Disease Resistance

Performance of Transgenic Chrysanthemum Harbouring Wasabi Defensin Gene for White Rust Disease Resistance

Standardized Genetic Transformation Protocol for Chrysanthemum cv. ‘Jinba’ with TERMINAL FLOWER 1 Homolog CmTFL1a

Breeding of the transgenic chrysanthemum (Chrysanthemum morifolium Ramat.) carrying aphid-resistance gene, Pinellia ternata agglutinin (PTA)

Contact Info

Product

Resources

About