Effect of SDF-1 with biphasic ceramic-like bone graft on the repair of rabbit radial defect

Wang, Fuke; Yang, Guiran; Yu, Xiao; He, Chuan; Cai, Guofeng; Song, En; Liu, Yanlin

doi:10.1186/s13018-019-1277-8

Cited by 6 publications

(6 citation statements)

References 26 publications

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“…Among all the groups, the PCL@BG20-GelMA/SDF-1α group showed the largest amount and greatest thickness of new bone, which was consistent with the results of micro-CT analysis. These results indicate that the addition of SDF-1α can promote the aggregation of rBMSCs, ensure that bone defect sites have a sufficient cell supply, and promote the rapid formation of new bone tissue, which was also consistent with the findings of Wang et al [ 37 ]. Wang et al [ 37 ] found that scaffolds carrying SDF-1α exhibited better bone conduction and induction ability.…”

Section: Resultssupporting

confidence: 91%

3D-printed PCL@BG scaffold integrated with SDF-1α-loaded hydrogel for enhancing local treatment of bone defects

Wang,

Dong,

Liu

et al. 2024

J Biol Eng

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Background The long-term nonunion of bone defects is always a difficult problem in orthopaedics treatment. Artificial bone implants made of polymeric materials are expected to solve this problem due to their suitable degradation rate and good biocompatibility. However, the lack of mechanical strength, low osteogenic induction ability and poor hydrophilicity of these synthetic polymeric materials limit their large-scale clinical application. Results In this study, we used bioactive glass (BG) (20%, W/W) and polycaprolactone (PCL, 80%, W/W) as raw materials to prepare a bone repair scaffold (PCL@BG20) using fused deposition modelling (FDM) three-dimensional (3D) printing technology. Subsequently, stromal cell-derived factor-1α (SDF-1α) chemokines were loaded into the PCL@BG20 scaffold pores with gelatine methacryloyl (GelMA) hydrogel. The experimental results showed that the prepared scaffold had a porous biomimetic structure mimicking that of cancellous bone, and the compressive strength (44.89 ± 3.45 MPa) of the scaffold was similar to that of cancellous bone. Transwell experiments showed that scaffolds loaded with SDF-1α could promote the recruitment of bone marrow stromal cells (BMSCs). In vivo data showed that treatment with scaffolds containing SDF-1α and BG (PCL@BG-GelMA/SDF-1α) had the best effect on bone defect repair compared to the other groups, with a large amount of new bone and mature collagen forming at the bone defect site. No significant organ toxicity or inflammatory reactions were observed in any of the experimental groups. Conclusions The results show that this kind of scaffold containing BG and SDF-1α serves the dual functions of recruiting stem cell migration in vivo and promoting bone repair in situ. We envision that this scaffold may become a new strategy for the clinical treatment of bone defects.

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Section: Resultssupporting

confidence: 91%

3D-printed PCL@BG scaffold integrated with SDF-1α-loaded hydrogel for enhancing local treatment of bone defects

Wang,

Dong,

Liu

et al. 2024

J Biol Eng

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“…Defect non-union represents the most dramatic clinical scenario, once the surgeon has to deal with a critical sized bone gap, and just few options are available for modify the devastated biological and mechanical environments (GIANNOUDIS;MARSH, 2007). Numerous biomaterials have been investigated in the field of bone tissue engineering and the critical sized bone defect has been the most common and effective experimental model for testing then (CASTAÑEDA et al, 2006;NIELSEN;KARRING;GOGOLEWSKI, 1992;NIEMEYER et al, 2010;WANG et al, 2019;ZHANG et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

“…Several studies have shown that using a radial ostectomy without cutting the ulna allows early and full limb function in rabbits, which is remarkably desirable (GEIGER et al, 2007;NIEMEYER et al, 2010;ZHAO et al, 2016). The bone region may vary between diaphyseal or metaphyseal, being the first one most commonly used to perform critical size defects in rabbits (CSD) (MA et al, 2018;WANG et al, 2019;XIE et al, 2017;ZHANG et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

A Critical Size Defect Model in the Radius of Rabbits

Minto¹,

Neto²,

Sprada³

et al. 2020

Ars Vet

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This study aimed to characterize a preclinical model of critical size defect (CSD) in the radius of rabbits.Twenty adults (> 7 months), female, New Zealand Rabbits, weighing between 3,5 to 5 Kg were used. They underwent a 1.5 cm long ostectomy of the diaphysis of the radius, starting 2 cm from the carpus joint. Radiographic analyses were performed at 15, 30, 60, and 90 days postoperatively, in order to evaluated bone callus formation, periosteal reaction, and bone bridge formation.The methodology allowed to precisely create bone defects of 1.5cm. Over time, no bone deposition was found at 15 days, but mild bone callus formation was observed in three animals after 60 days postoperative. At 90 days postoperatively only one rabbit presented bone consolidation radiographically, the others presented non-union. The critical bone defect proposed in this study was satisfactory, feasible with very low risk of complications.

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“…Subsequently, an ascending series of alcohol rinses were used for sample dehydration, and specimens were embedded in paraplast (Sherwood Medical Industries, Deland, FL, USA). Embedded samples were sectioned to a 5 μm thickness with a microtome (Cambridge Instruments, Germany), and slides were stained with hematoxylin and eosin (H&E), Masson-Goldner trichrome (GT), and Van Gieson’s (VG) for microscopic observation ( 27 , 28 ). In the VG microscopic images, the new bone area was measured for quantitative analysis with the Image J program (National Institute of Health, Stapleton, NY, United States).…”

Section: Methodsmentioning

confidence: 99%

Application of modified porcine xenograft by collagen coating in the veterinary field: pre-clinical and clinical evaluations

Jo,

Jang,

Shim

et al. 2024

Front. Vet. Sci.

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IntroductionThis study aimed to identify a collagen-coating method that does not affect the physicochemical properties of bone graft material. Based on this, we developed a collagen-coated porcine xenograft and applied it to dogs to validate its effectiveness.MethodsXenografts and collagen were derived from porcine, and the collagen coating was performed through N-ethyl-N’-(3- (dimethylamino)propyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) activation. The physicochemical characteristics of the developed bone graft material were verified through field emission scanning electron microscope (FE-SEM), brunauer emmett teller (BET), attenuated total reflectance-fourier transform infrared (ATR-FTIR), and water absorption test. Subsequently, the biocompatibility and bone healing effects were assessed using a rat calvarial defect model.ResultsThe physicochemical test results confirmed that collagen coating increased bone graft materials’ surface roughness and fluid absorption but did not affect their porous structure. In vivo evaluations revealed that collagen coating had no adverse impact on the bone healing effect of bone graft materials. After confirming the biocompatibility and effectiveness, we applied the bone graft materials in two orthopedic cases and one dental case. Notably, successful fracture healing was observed in both orthopedic cases. In the dental case, successful bone regeneration was achieved without any loss of alveolar bone.DiscussionThis study demonstrated that porcine bone graft material promotes bone healing in dogs with its hemostatic and cohesive effects resulting from the collagen coating. Bone graft materials with enhanced biocompatibility through collagen coating are expected to be widely used in veterinary clinical practice.

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Effect of SDF-1 with biphasic ceramic-like bone graft on the repair of rabbit radial defect

Cited by 6 publications

References 26 publications

3D-printed PCL@BG scaffold integrated with SDF-1α-loaded hydrogel for enhancing local treatment of bone defects

3D-printed PCL@BG scaffold integrated with SDF-1α-loaded hydrogel for enhancing local treatment of bone defects

A Critical Size Defect Model in the Radius of Rabbits

Application of modified porcine xenograft by collagen coating in the veterinary field: pre-clinical and clinical evaluations

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