Effect of Scutellarin inhibits collagen-induced arthritis through TLR4/NF-κB-mediated inflammation

Abstract

Scutellarin is the major effective constituent of the commonly used Chinese medicine Erigeron breviscapus. It has been applied in the clinic to treat various diseases, and is characterized by high content, a stable source, controllable quality, high efficiency and low toxicity. In addition, its potential pharmacological effects have been increasingly identified and elucidated. The present study was performed to examine the role of scutellarin on collagen‑induced arthritis (CIA). Mice were injected subcutaneously with bovine collagen type II and administered scutellarin for 2 weeks by gavage 20 mg/kg/day. ELISA kits were used to measure the levels of interleukin (IL)‑1β, IL‑6, tumor necrosis factor‑α (TNF‑α), oxidative stress markers [superoxide dismutase (SOD) and malondialdehyde (MDA)] and caspase‑3/-9 activity. Bax, Bcl‑2, toll like receptor 4 (TLR4) and nuclear factor (NF)‑κB protein expression was analyzed using western immunoblot analyses. The present study demonstrated that scutellarin prevented CIA, and inhibited the expression of inflammation factors, IL‑1β, IL‑6 and TNF‑α. In addition, scutellarin reduced the levels of oxidative stress markers, SOD and MDA, as well as intercellular adhesion molecule‑1 and monocyte chemoattractant protein 1 in CIA mice. Caspase‑3/-9, Bax/Bcl‑2, TLR4 and NF‑κB protein expression were reduced in CIA mice following scutellarin treatment. The results of the current study suggest a novel effect of scutellarin involving the inhibition of TLR4/NF‑κB‑mediated inflammation.

Scutellarin Attenuates the IL-1β-Induced Inflammation in Mouse Chondrocytes and Prevents Osteoarthritic Progression

“…Previous work has shown that the ability of SCU to inhibit NF-kB activity is linked to the AKT/NF-kB, p38/c-Jun Nterminal kinase (JNK), and NF-kB/mitogen-activated protein kinase (MAPK) pathways (Zhang et al, 2017). However, Nrf2/ HO-1 signaling is also a key anti-oxidative stress response pathway in cells (Wang et al, 2018a), with Nrf2 targeting HO-1, which in turn mediates anti-inflammatory (Tenhunen et al, 1968;Kim et al, 2007;Hsu et al, 2014).…”

Loganin attenuates intestinal injury in severely burned rats by regulating the toll‑like receptor 4/NF‑κB signaling pathway

“…Loganin suppresses severe burn-induced TLR4/NF-κB signaling pathway activation in intestinal tissues. The TLR/NF-κB signaling pathway plays an important role in inflammation and oxidative stress (9,10). Hence, the present study investigated whether loganin could inhibit burns-induced TLR4/NF-κB signaling pathway activation.…”

“…It has become increasingly clear that the inflammatory response and oxidative stress play crucial roles in the pathological process of burn injury (8). Toll-like receptor 4 (TLR4) is an innate immunity pattern recognition receptor, which initiates the immune response and oxidative damage by activating NF-κB, a crucial proinflammatory transcription factor (9,10). Therefore, it has been proposed that therapeutic targets that abolish intestinal inflammation and oxidative stress may function by mediating the TLR4 signaling pathway (11).…”

Antibiofilm activity of flavonoids on staphylococcal biofilms through targeting BAP amyloids

“…Two 19 mm intravenous catheters (24G; B. Braun) were implanted into the subcutaneous interscapular of each mouse and inoculated with 100 µl of S. aureus V329. Polyphenols were administered subcutaneously at the interscapular space during 10 days at non-toxic concentrations of QC 100 mg/kg, MC 20 mg/kg and SC 17.5 mg/kg 65 – 68 . After 10 days, animals were anesthetized by isoflurane inhalation followed by euthanatized by cervical dislocation.…”