Effect of Salmonella typhimurium infection on rat's cell oxidation and in vivo antioxidant activity of Vitellaria paradoxa and Ludwigia abyssinica aqueous extract

Fodouop, Siméon Pierre Chegaing; Gatsing, Donatien; Tangue, Benjamin Talom; Tagne, Richard Simo; Tala, Sédric Donald; Tchoumboué, J.; Kuiaté, Jules Roger

doi:10.1016/s2222-1808(14)60624-1

Cited by 11 publications

(8 citation statements)

References 16 publications

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“…Homogenates of various organs were prepared at 15% (15 g per 100 ml of body) [9], in Tris/HCl Buffer (pH7.2). This was done by grinding 600 mg of organ in 4 ml of buffer.…”

Section: Methodsmentioning

confidence: 99%

“…Although no scientific work has not yet been done on antibacterial activity of V. paradoxa leaves extract, the antibacterial activity of molecules isolated from its root bark have recently been reported [8]. In the West region of Cameroon, leaves and stem bark of V. paradoxa are used for the treatment of skin diseases, rheumatism, typhoid fever and microfilaria (in association with other medicinal plants) [9]. Keeping in view the traditional uses and previous work done in vitro on this plant, the present study proposed to investigate the in vivo antibacterial activity of its aqueous leaf extract against Salmonella typhimurium- infected rats.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Vitellaria paradoxa (C.F. Gaertn.) aqueous leaf extract administration on Salmonella typhimurium-infected rats

Fodouop

Tala

Keilah

et al. 2017

BMC Complement Altern Med

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BackgroundThe present study investigates the effects of Vitellaria paradoxa crude extract administration on Salmonella typhimurium infected Wistar rats.MethodsRats were infected by single dose oral administration of Salmonella typhimurium (1.5×108 CFU). Negative control groups were infected and treated orally with distilled water (vehicle), neutral control group were not infected, while the four test groups were treated up to 18 days with 55 mg/kg, 110 mg/kg, 220 mg/kg and 440 mg/kg body weight of aqueous extract of V. paradoxa respectively. The effects of this extract administration on serum markers (total protein, creatinine, transaminases, bilirubin and lipid profile) as well as acute toxicity test and phytochemical screening were also investigated.ResultsFollowing in vivo studies, aqueous extract of V. paradoxa allowed to clear salmonellosis in previously infected rats within twelve days of treatment. Infection has resulted in a significant increase of transaminases activity. Besides, significant decrease was observed in liver and kidney relative weight and their protein content. Nevertheless, administration of this plant extract at higher doses has resulted in the correction of some of these injuries. Results obtained from acute toxicity study showed that mice administered with the aqueous leaf extract exhibited a mild reaction to noise and pinch; excreted watery discharges and the LD50 value was 12.0 g/kg. In addition, the extract showed no toxic effect after 14 days. However, it may have a sedative effect or depressant effect on the central nervous system, may induce a decrease in plasma levels of algogenic substances, and may cause diarrhea at high doses. Phytochemical screening of the extract revealed the presence of flavonoids, alkaloids, tannins, phenols and polyphenols, saponins, anthocyanins, steroids and anthraquinones.ConclusionsThese results support the ethnomedicinal use of V. paradoxa, and suggest that its leave can be used in the management antibacterial phytomedicine.

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“…Homogenates of various organs were prepared at 15% (15 g per 100 ml of body) [9], in Tris/HCl Buffer (pH7.2). This was done by grinding 600 mg of organ in 4 ml of buffer.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Vitellaria paradoxa (C.F. Gaertn.) aqueous leaf extract administration on Salmonella typhimurium-infected rats

Fodouop

Tala

Keilah

et al. 2017

BMC Complement Altern Med

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“…Previous studies reported that triterpene possesses anti-inflammatory, antioxidation, anti-allergies, and antipruritic effects as well as improved diabetic nephropathy. [15][16][17] Documented studies of triterpene have shown reduced inflammation in patients with osteoarthritis, joint pain, cartilage disintegration and bone loss. 18 A previous study reported that in histological evidence, the triterpene from shea nut extract attenuated knee OA development in the standard-fed (normal) diet rat model.…”

Section: Introductionmentioning

confidence: 99%

<p><em>Vitellaria paradoxa</em> Nut Triterpene-Rich Extract Ameliorates Symptoms of Inflammation on Post-Traumatic Osteoarthritis in Obese Rats</p>

Sudirman¹,

Chen²,

Long³

et al. 2020

JPR

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Purpose: To investigate the ameliorative effects of Vitellaria paradoxa (VP) nut extract for an anterior cruciate ligament transection with medial meniscectomy (ACLT+MMx)-induced osteoarthritis (OA) in high-fat diet (HFD)-induced obese rats. Methods: The rats were fed by HFD for 5 weeks before surgery-induced OA. Rats were treated orally with three different doses of VP nut extract (111.6, 223.2, and 446.4 mg/kg) for 8 weeks. Results: The VP nut triterpene-rich extract decreased the level of triglycerides and increased high-density lipoprotein-cholesterol. The level of nitric oxide, interleukin (IL)-1β, IL-6, and tumor necrosis factor-α decreased after treatment with VP nut triterpene-rich extract, especially in high-doses. The VP nut triterpene-rich extracts also alleviated swelling in the knee OA, weight-bearing difference, and suppressed cartilage degradation. Conclusion:The Vitellaria paradoxa nut triterpene-rich extract suppressed proinflammatory mediators and attenuated the cartilage degradation and pain in osteoarthritis with an obesity rat model. As such, Vitellaria paradoxa nut triterpene-rich extract can be used as an alternative for osteoarthritis treatment.

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“…The lipid peroxidation index by the measurement of malondialdehyde (MDA) was measured in tissues using thiobarbituric acid (TBA) according to the method of [29], with certain modifications, malondialdehyde is one of the final products of the decomposition of polyunsaturated fatty acids (PUFAs) under the effect of free radicals released during stress. In a hot acidic medium (pH 2 to 3; 100°C), an MDA molecule condenses with two thiobarbituric molecules (TBA) to form a pink colored complex (reading at 532 nm).…”

Section: Determination Of Malondialdehyde (Mda)mentioning

confidence: 99%

In vitro and in vivo Antioxidant potential of aqueous and hydroethanolic extracts leaves ofDracaena deistelianaEng. (Dracacenaceae)

Feudjio

Goka

Josias

et al. 2023

Preprint

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Enterobacteria such as those of the genus Salmonella express the oxyR gene that codes for several proteins that allow it to resist free radicals. This resistance of Salmonella is often at the origin of an overproduction of these free radicals that can lead to oxidative stress. To investigate the antioxidant activity in vitro (aqueous and hydroethanolic extracts in vivo (of the 55% hydroethanolic extract) of Dracaena deisteliana leaves in albino rats of Wistar strain previously infected with the Salmonella strain Typhi ATCC 6539. The in vitro antioxidant activity of these extracts was determined by studying their anti-radical power with DPPH•, then their iron-reducing power and determining their flavonoids and total phenols content. In vivo antioxidant activity was determined in plasma and tissues of albino rats of wistar strain aged between 8 and 10 weeks previously infected with Salmonella typhi ATCC 6539. These infected rats concurrently received daily doses of Dracaena deisteliana extract (10, 20 and 51.84 mg/kg) or ciprofloxacin (14 mg/kg) as positive control, for 15 days. At the end of the treatment period, the animals were sacrificed and blood, liver, kidney, heart, lung, spleen testis and ovaris were collected for evaluation of antioxidants parameters, which included malondialdehyde, nitric oxide, catalase and peroxidase, as well as biological responses. Regarding in vitro antioxidant tests, the 65% hydroethanolic extract showed an anti-radical activity with DPPH• superior to that of all hydroethanolic extracts at 100 μg/ml. However, the infused showed lower antiradical activity than all extracts at 12.5 and 200 μg/ml concentrations. The 55% hydroethanolic extract had the best IC50of (11.99 μg/ml). The iron reducing power of this extract was higher than the other extracts at 200 μg/ml. The highest levels of flavonoids and total phenols were obtained respectively with the 55% and 95% hydroethanolic extract. The hydroethanol extract of Dracaena deisteliana (10, 20 and 51.84 mg/kg) cured the infected rats between the 9th and 13th day of treatment. The extract also significantly reduced (p < 0.05) blood malondialdehyde and nitric oxide levels, and significantly increased (p >0.05) the activity of catalase and peroxidase in the infected rats. The results suggest that leaves extract of Dracaena deisteliana contains antisalmonella and antioxidant substances, which could be used for the treatment of typhoid fever and another salmonellosis. In addition, 55% hydroethanolic extract of this plant possesses antisalmonella activity and reduces the state of oxidative stress caused by S. typhi during rat's infection.

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Effect of Salmonella typhimurium infection on rat's cell oxidation and in vivo antioxidant activity of Vitellaria paradoxa and Ludwigia abyssinica aqueous extract

Cited by 11 publications

References 16 publications

Effects of Vitellaria paradoxa (C.F. Gaertn.) aqueous leaf extract administration on Salmonella typhimurium-infected rats

Effects of Vitellaria paradoxa (C.F. Gaertn.) aqueous leaf extract administration on Salmonella typhimurium-infected rats

<p><em>Vitellaria paradoxa</em> Nut Triterpene-Rich Extract Ameliorates Symptoms of Inflammation on Post-Traumatic Osteoarthritis in Obese Rats</p>

In vitro and in vivo Antioxidant potential of aqueous and hydroethanolic extracts leaves ofDracaena deistelianaEng. (Dracacenaceae)

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