Effect of S53P4 bioactive glass and low-level laser therapy on calvarial bone repair in rats submitted to zoledronic acid therapy

Abstract

To evaluate the influence of bioactive glass and photobiomodulation therapy (PBMT) in calvarial bone repair process in rats submitted to zoledronic acid therapy. Methods: Twenty-four rats were selected and treated with the dose of 0.035 mg/kg of zoledronic acid every two weeks, totalizing eight weeks, to induce osteonecrosis. After the drug therapy, surgical procedure was performed to create 5-mm diameter parietal bone defects in the calvarial region. The rats were then randomly assigned to groups according to the following treatments: AZC: control group, treated with blood clot;

β-TCP/S53P4 Scaffolds Obtained by Gel Casting: Synthesis, Properties, and Biomedical Applications

“…At 21 days, the β-TCP/S53P4 scaffolds induced greater bone formation in the defect area, presenting a significant difference (p < 0.05) when compared to β-TCP. Previous studies have proven the osteoconductive properties of this bioglass [78,79]. Signs of bone remodeling in defects filled with β-TCP scaffolds were observed in this study and agree with previous studies [7,8].…”

Influence of zoledronic acid and low-intensity laser on collagen fibers during the bone repair process

“…The bone defect is considered critical when it is not completely filled with bone tissue; in rat tibias, defects equal to or greater than 2 mm in diameter are considered critical 3 , 14 . Research carried out to evaluate the bone repair process in critical bone defects submitted to laser therapy associated with ZA is scarce in the literature 3 , 10 , 11 .…”

“…In the literature, there is little research evaluating the association of zoledronic acid and laser therapy in the bone repair process 3 , alveolar 10 , and with biomaterials 11 . Additionally, none of the aforementioned studies evaluated the quality and quantity of collagen fibers, which are directly related to the bone repair process.…”

The additive effects of bioactive glasses and photobiomodulation on enhancing bone regeneration

“…Recently, some studies combined biomaterials with PBM and have achieved positive results in tissue regeneration [ 48–50 ]. However, some other studies showed that the superiority of PBM cannot be exploited when combined with biomaterials, which may be related to the excessive stimulation of tissue caused by the inappropriate dosage of biomaterials and PBM [ 51 ].…”