Effect of ruminal ammonia supply on lysine utilization by growing steers1

Hussein, Ali H.; Batista, Erick Darlisson; Miesner, Matt D.; Titgemeyer, Evan C.

doi:10.2527/jas.2015-9717

Cited by 4 publications

(5 citation statements)

References 41 publications

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“…with P-LYS supplementation in the growth study is sequential to lower serum urea in that period which indicating that lysine was used for protein deposition and hence reduced AA catabolism which appeared as reduction in serum urea (Hussein et al, 2016). These results agree with Hussein et al (2016) who found that lysine infusion reduced serum urea. He also found no effect of abomasal infusion of lysine on blood glucose or insulin in growing steers.…”

Section: Table (3): Effects Of Free Lysine (F-lys) and Protected Lysisupporting

confidence: 81%

“…Also, unchanged digestibility of DM, OM, CP, ADF, NDF, cellulose and hemicellulose (Table 6) in response to P-LYS supplementation indicated that P-LYS did not affect ruminal fermentation and nutrient digestibility in the concentration used in the current study. In accordance to these results, Hussein et al (2016) reported no effect of infused lysine in OM and DM digestibility of diet-deficient in lysine when given to growing steers. Elwakeel et al (2012) reported that high concentrations of P-LYS that were used in-vitro had negative effect on ruminal fermentation when used at concentrations of 25 and 50 mM.…”

Section: Table (4): Effects Of Free Lysine (F-lys) and Protected Lysisupporting

confidence: 75%

“…Therefore, lysine was used for protein deposition. Many studies have shown that lysine supplementation either in protected form or abomasally infused, increased NB and reduced urine N when added to diets that are deficient in lysine (Oke et al, 1986;Brookes et al, 1973;Nimrick et al, 1970 andHussein et al, 2016). The tendency to improve RN as percent of NI (P=0.05) is emphasized in the improvement in ADG in the last 2 wk.…”

Section: Nitrogen Intake Feces and Urine N N Balance And Retained Nmentioning

confidence: 99%

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Effect of Rumen-Protected Lysine (Hydroxy Methyl Lysine) Supplementation on Performance and Blood Metabolites in Barki Sheep

Elwakeel

El-Zaiat

Morsy

2018

Egyptian Journal of Nutrition and Feeds

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ffect of rumen-protected lysine in the form of Hydroxyl methyl lysine (HML) on average daily gain (ADG), dry matter intake (DMI), nutrient digestibility, nitrogen balance blood metabolites, were evaluated in two experiments using growing Barki lambs. In first experiment, fifteen lambs were housed individually and assigned into three equal groups fed on basal diet (no lysine-control), free lysine (positive control, F-LYS) and protected lysine (P-LYS) equivalent to 3 g/d of lysine. The experiment lasted for 10 week to evaluate feed intake, ADG and blood metabolites. No effect (P>0.05) of treatment on ADG, DMI and feed efficiency was observed. Whereas, treatment × time interaction tendency (P=0.07) as showed an increased in ADG as lysine supplemented as P-LYS compared to F-LYS (P=0.05) and control (P<0.05) at the last 2 weeks. No effect (P>0.05) of treatment was observed on serum total protein (TP), albumin (Alb), globulin (Glob), urea, glucose (G), cholesterol (CH) and triglycerides (TG) concentrations. However, treatment × time interaction (P=0.06) tendency existed as P-LYS increased (P<0.05) TP, Alb and Glob only at wk. 2 and reduced (P<0.05) serum urea only at the last 2 weeks. Further, no treatment effect (P>0.05) was found on rumen ammonia nitrogen (NH 3-N), pH and protozoa numbers. In Exp.2, (digestibility trial), twelve lambs from Exp.1 were kept in metabolic cages and received their respective treatments for 7 days as collection period. No response (P>0.05) to P-LYS supplementation was observed on nutrient digestibility of dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fibre (NDF) and acid detergent fibre (ADF) compared to F-LYS and control. A tendency (P=0.09) for treatment on nitrogen balance (NB) and retained nitrogen (RN, P=0.06) was found. P-LYS increased (P=0.05) NB to 7.1±0.14 vs. 4.8±0.3g/d and RN (P<0.05) from 37.9 vs. 25.59% in the control. However, P-LYS did not improve lambs performance under the basal diet fed that might have not been deficient enough in LYS amino acid, the improved ADG and decreased plasma urea at the last two weeks along with the slight improvement in NB and RN indicates the P-LYS has a potential under specific feeding regimes.

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Section: Table (3): Effects Of Free Lysine (F-lys) and Protected Lysisupporting

confidence: 81%

Section: Table (4): Effects Of Free Lysine (F-lys) and Protected Lysisupporting

confidence: 75%

Section: Nitrogen Intake Feces and Urine N N Balance And Retained Nmentioning

confidence: 99%

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Effect of Rumen-Protected Lysine (Hydroxy Methyl Lysine) Supplementation on Performance and Blood Metabolites in Barki Sheep

Elwakeel

El-Zaiat

Morsy

2018

Egyptian Journal of Nutrition and Feeds

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“…In the current study, while BUN differences were insignificant, BUN concentrations at 4–6 weeks postpartum in the CON group were higher than those in the RPL group ( p = .120). BUN reduction by RPL supplementation is already known (Hussein et al., 2016; Kamiya et al., 2021; Zou et al., 2023), and a similar mechanism may have been involved in this study. Yet, as noted earlier, the cows in the RPL group probably had an AA imbalance before the commencement of the experiment.…”

Section: Discussionsupporting

confidence: 68%

“…Low‐protein diets supplemented with rumen‐protected lysine (RPL) and methionine reduced blood urea nitrogen (BUN) levels in steers (Kamiya et al., 2021) and bulls (Zou et al., 2023). Infusing ammonia into the rumen and lysine into the abomasum has led to reduced urea in urine and increased retained protein in steers (Hussein et al., 2016), suggesting that lysine supplementation improves nitrogen use efficiency. The negative effect of high‐protein diets on the reproductive performance of dairy cows is attributed to elevated blood ammonia and BUN levels, which affect the uterus and ovaries (Ferguson & Chalupa, 1989; Tamminga, 2006).…”

Section: Introductionmentioning

confidence: 99%

Supplementation of rumen‐protected lysine during the close‐up period improves vaginal discharge clearance in Holstein dairy cows

Kawashima,

Hayakawa,

Taniguchi

et al. 2024

Reprod Domestic Animals

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We aimed to evaluate the effects of rumen‐protected lysine (RPL) supplementation during the close‐up period on uterine involution and the resumption of ovarian function in dairy cows. Fifty‐two multiparous Holstein cows were categorized based on parity and expected calving date and randomly assigned to the RPL or control (CON) groups. The RPL group received 80 g of RPL daily from day 21 before the expected calving date until parturition. Blood samples were obtained twice weekly from pre‐supplementation to 6 weeks postpartum. The onset of luteal activity postpartum was determined via ultrasonography twice weekly for up to 6 weeks postpartum. Uterine involution was tracked at 3 and 5 weeks postpartum through the vaginal discharge score, percentage of polymorphonuclear cells (PMN) in endometrial cytology samples, presence of intrauterine fluid, and gravid horn diameter via ultrasonography. Before supplementation, the RPL group showed amino acid imbalance, which was improved by RPL supplementation. There were no significant differences in the onset of luteal activity, percentage of PMN, intrauterine fluid, or the diameter of the uterine horn between the two groups. The vaginal discharge score in the RPL group decreased from 3 to 5 weeks postpartum, whereas that in the CON groups did not decrease. The number of cows with clinical endometritis was lower in the RPL group. Overall, RPL supplementation during the close‐up period enhanced vaginal discharge clearance, potentially averting clinical endometritis, but did not affect the first ovulation in dairy cows.

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