Effect of Rumen-Protected Lysine (Hydroxy Methyl Lysine) Supplementation on Performance and Blood Metabolites in Barki Sheep

Abstract

ffect of rumen-protected lysine in the form of Hydroxyl methyl lysine (HML) on average daily gain (ADG), dry matter intake (DMI), nutrient digestibility, nitrogen balance blood metabolites, were evaluated in two experiments using growing Barki lambs. In first experiment, fifteen lambs were housed individually and assigned into three equal groups fed on basal diet (no lysine-control), free lysine (positive control, F-LYS) and protected lysine (P-LYS) equivalent to 3 g/d of lysine. The experiment lasted for 10 week to evaluate feed intake, ADG and blood metabolites. No effect (P>0.05) of treatment on ADG, DMI and feed efficiency was observed. Whereas, treatment × time interaction tendency (P=0.07) as showed an increased in ADG as lysine supplemented as P-LYS compared to F-LYS (P=0.05) and control (P<0.05) at the last 2 weeks. No effect (P>0.05) of treatment was observed on serum total protein (TP), albumin (Alb), globulin (Glob), urea, glucose (G), cholesterol (CH) and triglycerides (TG) concentrations. However, treatment × time interaction (P=0.06) tendency existed as P-LYS increased (P<0.05) TP, Alb and Glob only at wk. 2 and reduced (P<0.05) serum urea only at the last 2 weeks. Further, no treatment effect (P>0.05) was found on rumen ammonia nitrogen (NH 3-N), pH and protozoa numbers. In Exp.2, (digestibility trial), twelve lambs from Exp.1 were kept in metabolic cages and received their respective treatments for 7 days as collection period. No response (P>0.05) to P-LYS supplementation was observed on nutrient digestibility of dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fibre (NDF) and acid detergent fibre (ADF) compared to F-LYS and control. A tendency (P=0.09) for treatment on nitrogen balance (NB) and retained nitrogen (RN, P=0.06) was found. P-LYS increased (P=0.05) NB to 7.1±0.14 vs. 4.8±0.3g/d and RN (P<0.05) from 37.9 vs. 25.59% in the control. However, P-LYS did not improve lambs performance under the basal diet fed that might have not been deficient enough in LYS amino acid, the improved ADG and decreased plasma urea at the last two weeks along with the slight improvement in NB and RN indicates the P-LYS has a potential under specific feeding regimes.

The Effect of Adding Protected Amino Acids in the Rumen (Methionine and Lysine and their Mixtures) on Some Physiological Characteristics of Male Arabi Lambs

“…The means of cholesterol, triglyceride, ALT and AST concentration were (87.00, 76.28, 85.40, 88.52) mg / 100 ml, and (63.49, 57.98, 60.11, 62.99) mg / 100 mL and ALT (14.64,15.51,16.91,14.84) IU / L, AST (40.71,42.22,43.37,41.33) IU / L, respectively. The results were in agreement with the results of Mousavi et al (2016) and Elwakeel et al (2018) when adding amino acids (methionine and lysine) to the diets of Iranian ewes and lambs of the Egyptian Barqi breed, which they did not recorded significant differences in the concentration of triglyceride and cholesterol. Whereas, it did not agree with the results of Rodriguez-Guerrero et al (2018) when using protected methionine amino acid, which reported significant differences in cholesterol and triglyceride concentrations.…”

“…Different small letter within column means significant difference (*) (p<0.05) between groups. Table (6) shows that there are significant differences (p <0.05) in the concentration of albumin in the fourth group, which recorded (3.81) g / 100 ml compared with the control group, reaching (3.20) g / 100 ml, may be due to increasing the absorption of the true protein in the small intestine when feeding the protected protein (Oh et al, 2008), and this is similar to the results of Gavade et a., (2019), that confirmed that a significant difference was obtained in albumin concentration when feeding calves on a mixture of the protected amino acids methionine and lysine (3 + 20 g / animal / day) when compared with the control group, whereas, it is different from the results of Elwakeel et a., (2018), which did not record significant differences in the albumin concentration compared to the control group when they added the protected amino acid lysine at (3) g / animal / day to the diets of lambs of the Egyptian Barqi breed. A significant (p <0.05) increase was observed in glucose concentration in the fourth and second groups, reaching (69.11, 68.43) mg / 100 ml, respectively, compared to the control group, reaching (43.85) mg / 100 ml, and the reason may be due to the metabolism of the amino acid methionine in the liver and its association with an increase in glucose concentration (Ganong, 2005;Sun et a., 2016) .…”

The effect of adding protected amino acids (methionine and lysine) on the performance and carcass characteristics of male Arabi lambs

“…As for the results of the study of Yahya and Abubakar (2012), when added protected methionine at different levels (0.2%, 0.4%/kg of dry matter) to the diets of fattening lambs where they noticed a significant increase in the average daily gain compared with the control group. Elwakeel et al (2018) did not record significant differences in the average daily gain when fed Barki lambs on diets contain 3g of protected lysine. Also, Abdelrahm (2010) did not find significant differences in the average total gain when fed Baladi goat breed on protected methionine (0, 2.5, 5) g/head/day.…”