Effect of Royal Jelly Treated with Protease on Blood Pressure in Spontaneously Hypertensive Rats

Abstract

Protease digested royal jelly (ProRJ) proved to inhibit angiotensin I converting enzyme (ACE) activity in vitro.And we have demonstrated that peptides isolated from ProRJ (Ile-Tyr (IY), Val-Tyr (VY)) also inhibited ACE activity.We examined an antihypertensive effect of RJ, ProRJ and RJ peptides (IY and VY) on spontaneously hypertensive rat (SHR). In chronic oral administration of ProRJ and RJ at the dose of 10 mg/kg/day for 28 days, the systolic blood pressure (SBP) of SHR was significantly decreased against the control.The blood pressure of normotensive rat (Wistar Kyoto rat) did not changed on the repeated administration of ProRJ.In repeated oral administration of RJ peptides at the dose of 10mg/kg/day for 28 days, SBP was significantly reduced against the control. These results suggest that the hypotensive effect of ProRJ is correlated with ACE inhibitory activity of RJ peptides, and ProRJ would be beneficial for improving blood pressure in hypertension.

Antihypertensive Effect of Peptides from Royal Jelly in Spontaneously Hypertensive Rats

“…We have shown that Protease N treated RJ (ProRJ) and peptides (IY, VY, IVY) from ProRJ inhibited angiotensin I-converting enzyme (ACE) activity 6) and they have an antihypertensive effect in repeated oral administration for 28 d on spontaneously hypertensive rats (SHR). 7) Their ACE inhibitory activity (IC 50 ) of IY, VY and IVY are 0.008, 0.020 and 0.018 (mg/ml) respectively. 5) Generally, the Renin-angiotensin (RA) system is well known to regulate blood pressure (BP) in the circulatory system and localized organs.…”

Utilization Study of Stems and Leaves of Tienchi Ginseng. I. Anti-Hypertensive Effect of Stems and Leaves of Tienchi Ginseng on Stroke-Prone Spontaneously Hypertensive Rat (SHRSP)

“…In this present study, no PPT was apparent in the aboveground parts of Tienchi ginseng. There have been many reports describing hypotensive effect of various food material, [9][10][11][12][13] but they are considered to be expensive and sometimes need special processing to y To whom correspondence should be addressed. Tel: +81-54-264-5526; Fax: +81-54-264-5528; E-mail: riceball 2@ybb.ne.jp Abbreviations: SHRSP, stroke-prone spontaneously hypertensive rat; WKY, Wistar Kyoto rat; PPD, 20(s)-protopanaxadiol; PPT, 20(s)-protopanaxatriol; GABA, -aminobutyric acid; SBP, systolic blood pressure; DBP, diastolic blood pressure; HR, heart rate; LAT, locomotive activity prepare the product.…”