Utilization Study of Stems and Leaves of Tienchi Ginseng. I. Anti-Hypertensive Effect of Stems and Leaves of Tienchi Ginseng on Stroke-Prone Spontaneously Hypertensive Rat (SHRSP)

Yanai, Kazuhiro; Sato, Kazumasa; Masuda, S.; Ikeda, Masahiko; Kinae, Naohide

doi:10.1271/bbb.60233

Cited by 2 publications

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SLPN was used to treat insomnia, anxiety, and neurasthenia neurosis . SLPN also elicits strong neuroprotective , antitumor , and anti‐hypertensive effects . In our previous study, our laboratory tested the bioactivities of constituents present in SLPN on cardiovascular diseases and found some bioactive compounds with effects on blood hemostasis or activating blood .…”

Section: Introductionmentioning

confidence: 99%

Analysis of bioactive components and multi‐component pharmacokinetics of saponins from the leaves of Panax notoginseng in rat plasma after oral administration by LC–MS/MS

Ju¹,

Li²,

Han³

et al. 2018

J of Separation Science

View full text Add to dashboard Cite

In this study, simple ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass tandem mass spectrometry is used to characterize the absorbed components in rat plasma after the oral administration of saponins from the leaves of Panax notoginseng. Seventeen prototype compounds are structurally characterized. Furthermore, a simple and sensitive liquid chromatography with tandem mass spectrometry method is also used for the simultaneous determination of notoginsenoside Fc, ginsenoside Rb1, ginsenoside Rc, ginsenoside Rb3, ginsenoside Rd, and notoginsenoside Fe in rat plasma within 5 min. After n-butanol mediated liquid-liquid extraction, all analytes were separated on a C column and monitored in negative ion mode. Linearity, sensitivity, intra- and inter-assay precision, accuracy, recovery, matrix effect, and stability were all within acceptable ranges. The validated liquid chromatography with tandem mass spectrometry method is successfully applied to the pharmacokinetic study of saponins from the leaves of Panax notoginseng in rats after oral administration. The results suggest that notoginsenoside Fc and ginsenoside Rb3 showed relatively higher exposure compared with other saponins. All saponins showed a long duration in plasma with a t longer than 15 h, except notoginsenoside Fe (t = 2.78 h). This study provides important information about the metabolism of saponins from the leaves of Panax notoginseng, which is useful for completely understanding its mechanism of action.

show abstract