Effect of rosmarinic acid as a chemopreventive modality on experimentally induced hamster buccal pouch carcinogenesis

Abstract

Aim: It was directed to evaluate rosmarinic acid (RA) effect as a chemopreventive modality on experimentally induced hamster buccal pouch (HBP) carcinogenesis. Material and methods: Forty Syrian male hamsters five weeks old, weighing 120-80g, were divided into four groups of ten hamsters in each as follows, GI: Only topical application of liquid paraffin (3 times a week for 14 weeks), GII: Topical application of 12 ,7 dimethyl benz[a]anthracene (DMBA) alone (%0.5 in liquid paraffin, 3 times a week for 14 weeks), GIII: Topical application of DMBA (%0.5 in liquid paraffin, 3 times a week for 14 weeks) associated by oral administration of RA (100mg/kg b.w in 1ml distilled water by oral gavage, 3 times a week for 14 weeks on opposite days of DMBA application), GIV : Oral administration of RA alone (100mg/kg b.w in 1ml distilled water by oral gavage, 3 times a week for 14 weeks). Results: Gross observation and histopathological findings revealed a-GI: normal stratified squamous epithelium b-GII: well and moderately differentiated SCC c-: GIII: revealed considerably reversed tumor incidence d: GIV: normal similar to GI. Immunohistochemical results revealed, oral administration of RA to DMBA treated hamsters restored the normal expression of bcl2-and Bax indicating that RA may have chemopreventive mechanism and/or pro-apoptotic effects. Conclusion: RA may act through different mechanisms, such as applying anti-inflammatory, antioxidant results as well as impede cell proliferation, migration, and selectively convince cancer cells apoptosis.

FOXD1-mTOR Signaling Pathway on Oral Squamous Cell Carcinoma and Its Inhibition by Rosemary Extract (Invitro-Study)

“…More results of RE apoptotic effects were documented in Abd El Wahab et al, (2020) study, evaluating the apoptotic effect of rosmarinic acid, which is also a component of RE to treated hamsters induced OSCC. This was clarified that rosmarinic acid may act through numerous mechanisms such as exerting anti-inflammatory and antioxidant effects, in addition to inhibiting cell proliferation, migration, and inducing cancer cells apoptosis.…”