Effect of risperidone on proliferation and apoptosis of MC3T3-E1 cells

Abstract

This aim of this study was to assess the molecular mechanism of osteoporosis in schizophrenia patients with risperidone use. Here, we investigated the effects of risperidone on cellular proliferation and apoptosis of a preosteoblast cell line, MC3T3-E1. Cell viability and apoptotic rate of MC3T3-E1 were detected by cell counting kit-8 and flow cytometry at a serial dose of risperidone and at different time points, respectively. Bone transformation relevant gene serum osteocalcin (BGP), collagen 1, tumor necrosis factor-α (TNF-α), osteoprotegerin (OPG), and receptor activator of nuclear factor-κB ligand (RANKL) mRNA levels were determined by real-time PCR (qPCR). Their protein expression patterns were evaluated using western blot. The results revealed that risperidone dramatically inhibited MC3T3-E1 cell proliferation in a dose-dependent manner. It also significantly induced MC3T3-E1 cell apoptosis. TNF-α gene and protein levels were greatly enhanced after risperidone treatment. In contrast, BGP, collagen 1, OPG, and RANKL gene and protein levels were markedly downregulated. Our study indicated that risperidone suppressed MC3T3-E1 cell proliferation and induced apoptosis. It also regulated BGP gene and protein expression.

Housing Temperature Influences Atypical Antipsychotic Drug‐Induced Bone Loss in Female C57BL/6J Mice

“…RIS suppresses MC3T3‐E1 cell osteoblast differentiation in a dose‐dependent manner. ( 50 ) However, dopamine also suppresses osteoblast differentiation, suggesting RIS‐mediated effects may not be through dopamine receptor inhibition. ( 5 ) Furthermore, RIS promotes osteoclast differentiation in vitro, which can also be blocked by dopamine.…”

“…Studies from cell lines and primary osteoblasts and osteoclasts indicate that RIS may also have direct effects on bone. RIS suppresses MC3T3-E1 cell osteoblast differentiation in a dosedependent manner (53) . However, dopamine also suppresses osteoblast differentiation, suggesting RIS-mediated effects may not be through dopamine receptor inhibition (5) .…”

Production and identification of peptides with activity promoting osteoblast proliferation from meat dregs of Pinctada martensii

“…Mouse preosteoblastic MC3T3‐E1 cells were purchased from the Shanghai cell bank of the Chinese Academy of Sciences (Shanghai, China), culturing in α‐MEM medium including 10% FBS and 1% penicillin–streptomycin in a CO 2 incubator which included 5% CO 2 at 37°C (Zheng et al., 2019).…”

The Synergistic Effect of Nicotine and Staphylococcus aureus on Peri-Implant Infections

“…For the nicotine and S. aureus –nicotine combination group, the culture medium was supplemented with 1 μM nicotine. Cells were harvested for RT-qPCR ( Widaa et al, 2012 ; Zheng et al, 2019 ).…”

“…The RT-qPCR were then proceeded following the SYBR® PremixEx TaqTM kit (Takara Inc.) two-step strategy: 1) 95°C for 30 s; 2) 40 PCR cycles (95°C for 5 s, a gene-specific annealing temperature for 30 s). All primer sequences used are listed in Table 1 ( Zheng et al, 2019 ), and 16S rDNA and β-actin were chosen as the reference genes. RT-qPCRs were run on LightCycler 480 II (Roche, Basel, Switzerland).…”