Effect of Rhaphidophora korthalsii methanol extract on human peripheral blood mononuclear cell (PBMC) proliferation and cytolytic activity toward HepG2

Yeap, Swee Keong; Alitheen, Noorjahan Banu; Ali, Abdul Manaf; Omar, Abdul Rahman; Raha, Abdul Rahim; Suraini, A.A.; Muhajir, A.H.

doi:10.1016/j.jep.2007.08.020

Cited by 42 publications

(49 citation statements)

References 22 publications

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“…Similar findings were obtained by other researchers. 38,39 We speculate that the decrease in cell viability with longer exposure periods could be partly attributed to the depletion of nutrients in the culture medium, which was not changed for the duration of the experimental period. This interpretation is supported by the observation that the control cells exhibited the same trend, indicating that the decrease was not totally drug induced.…”

Section: Discussionmentioning

confidence: 92%

The Effects ofSyzygium aromaticum-Derived Oleanolic Acid on Kidney Function of Male Sprague–Dawley Rats and on Kidney and Liver Cell Lines

et al. 2012

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Studies indicate that Syzygium spp-derived oleanolic acid (OA) enhances renal function of streptozotocin (STZ)-induced diabetic rats as evidenced by its reversal of the previously reported inability of the kidney to excrete Na þ in these animals. We postulated that OA influences Na þ excretion in the proximal tubule, the site where two-thirds of filtered NaCl is reabsorbed through a process mediated by transport proteins. Therefore, the study investigated the effects of OA on proximal tubular Na þ handling in male Sprague-Dawley rats using renal lithium clearance (C Li ). Renal C Li has been used widely in animal and clinical studies to assess proximal tubular function. Sub-chronic doses of OA were administered to rats twice every third day for 5 weeks. Rats treated with deionized water served as control animals. Cytotoxicity of OA on kidney and liver cell lines was assessed by the MTT and comet assays. OA increased Na þ excretion of conscious male Sprague-Dawley rats from week 3 to week 5. By the end of the 5-week experimental period, OA treatment significantly reduced (p < 0.05) plasma creatinine concentration of STZ-induced diabetic rats with a concomitant elevation in glomerular filtration rate (GFR). Acute OA infusion was also associated with increases in fractional excretion of sodium (FE Na ) and lithium (FE Li ) in anesthetized rats in the absence of significant changes in GFR. The MTT assay studies demonstrated that OA increased the metabolic activity of kidney and liver cell lines. Taken together with previous observations, this study implicates the proximal tubule in OA-evoked increases in urinary Na þ output.

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Section: Discussionmentioning

confidence: 92%

The Effects ofSyzygium aromaticum-Derived Oleanolic Acid on Kidney Function of Male Sprague–Dawley Rats and on Kidney and Liver Cell Lines

et al. 2012

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“…The cells migrating into the lower chamber were collected and then transferred to 96-well plates (Corning, USA). The number of cells was quantified as previously described [14].…”

Section: Chemotaxis Assaymentioning

confidence: 99%

Screening and Mechanism of Antagonist Peptide for CC Chemokine Receptor 1 (CCR1) Derived from Viral Macrophage Inflammatory Protein II

Li¹,

Ding²,

Wei³

et al. 2014

Trop. J. Pharm Res

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“…There are several plants have been recognized to have mitogenic effect on the immune cells. For examples, Rhaphidophora korthalsii was found to stimulate immune cell proliferation, cytokines expression and natural killer cell cytotoxicity in vitro and in vivo (Yeap et al, 2007;2011a;2011b). On the other, Elephantopus scaber and Vernonia amygdalina were found to be the major ingredient in a traditional herbal formula, which carries the immunomodulatory effect (Ho et al, 2009;Yeap et al, 2010;Hertiani et al, 2010).…”

Section: Plant Mitogensmentioning

confidence: 99%

Evaluation of Immunomodulatory Effect: Selection of the Correct Targets for Immunostimulation Study

Chun-Chu¹

2011

American Journal of Immunology

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Problem statement: Numerous plants or remedies that are traditionally used for various diseases had been claimed to maintain general good health, particularly the immune system. With the advanced understanding on immunology and ethnopharmacology, study on the interaction of this herb with the immune system is critical to understand the safety and its efficacy as a potent immunomodulatory agent. Approach: Selecting proper immune cells from a suitable immune organ allowed for the understanding on the mode of immunomodulation. Lymphocytes isolated from mammalian thymus, spleen and bone marrow represented great candidates for this immunomodulatory study. Results: A number of herbal extracts including that of Rhaphidophora korthalsii and compounds isolated from this plant, namely lectin and zerumbone, had been identified as potent immunostimulators. Conclusion/Recommendations: Future studies are needed to elucidate the mechanism underlaying activites of these immunomodulatory agents.

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Effect of Rhaphidophora korthalsii methanol extract on human peripheral blood mononuclear cell (PBMC) proliferation and cytolytic activity toward HepG2

Cited by 42 publications

References 22 publications

The Effects ofSyzygium aromaticum-Derived Oleanolic Acid on Kidney Function of Male Sprague–Dawley Rats and on Kidney and Liver Cell Lines

The Effects ofSyzygium aromaticum-Derived Oleanolic Acid on Kidney Function of Male Sprague–Dawley Rats and on Kidney and Liver Cell Lines

Screening and Mechanism of Antagonist Peptide for CC Chemokine Receptor 1 (CCR1) Derived from Viral Macrophage Inflammatory Protein II

Evaluation of Immunomodulatory Effect: Selection of the Correct Targets for Immunostimulation Study

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