Effect of resveratrol on proliferation and apoptosis of human pancreatic cancer MIA PaCa-2 cells may involve inhibition of the Hedgehog signaling pathway

Abstract

Abstract. We previously demonstrated that resveratrol (Res) regulates the expression of PKC α and δ, to eventually inhibit growth and induce apoptosis in human gastric cancer cells. In the present study, the effect of Res on the growth of human pancreatic cancer cells was investigated, to further unveil the underlying mechanism. The human pancreatic cancer cell line MIA PaCa-2 was treated with three different concentrations of Res. Cell proliferation was assessed by the MTT assay, and apoptosis was detected by flow cytometry. Reverse transcription-quantitative PCR (RT-qPCR) and western blot analysis were used to measure the mRNA and protein levels of the Hedgehog (Hh) signaling proteins Ihh, Ptch and Smo. Our results revealed that Res can inhibit the cell proliferative ability in a time-and dose-dependent manner. The number of formed colonies in the Res-and 5-Fu (positive control)-treated groups was decreased as compared to the negative control group. Res further induced apoptosis of MIA PaCa-2 cells in a dose-dependent manner. In addition, the levels of Ihh, Ptch and Smo were decreased by Res treatment. Our findings suggest that Res inhibits proliferation and induces apoptosis of MIA PaCa-2 pancreatic cancer cells in vitro, which may be related to its inhibitory effect on the Hh signaling pathway.

Resveratrol inhibits hypoxia-driven ROS-induced invasive and migratory ability of pancreatic cancer cells via suppression of the Hedgehog signaling pathway

“…Our previous study demonstrated that resveratrol plays an important role in suppressing the proliferation and EMT of pancreatic cancer cells via the PI-3K/Akt/NF-κB signaling pathway (22). In addition, we also confirmed that resveratrol inhibited the growth of human pancreatic cancer cells in vitro by inhibiting cell proliferation and promoting cell apoptosis via inhibition of the Hh signaling pathway (23).…”

YAP Inhibition by Resveratrol via Activation of AMPK Enhances the Sensitivity of Pancreatic Cancer Cells to Gemcitabine

“…Over the past several years, many reports have demonstrated that resveratrol acts as a cancer chemo-preventive agent to induce growth inhibition, cell cycle arrest, apoptosis, and changes in biomarker expression in several human cancer cell lines [19,20,21]. Our previous study showed that resveratrol plays an important role in suppressing the proliferation of pancreatic cancer cells via the PI-3K/Akt/NF-κB signaling pathway [22] and the hedgehog signaling pathway [23,24]. However, whether resveratrol can inhibit YAP expression and its molecular mechanism have not been elucidated.…”

Daucosterol inhibits cancer cell proliferation by inducing autophagy through reactive oxygen species-dependent manner

“…Bioassay-guided analysis of ethyl acetate fraction identified eight compounds, including epigallocatechin-3-gallate (ECCG), kaempferol, naringenin, beta-sitosterol, resveratrol, taxifolin and daucosterol. The anti-cancer effects of ECCG [5,6], kaempferol [7,8], naringenin [9,10], beta-sitosterol [11,12], resveratrol [13,14] and taxifolin [15] were all reported previously. Daucosterol is not endogenously synthesized but can be obtained through diet.…”