Effect of relative humidity on the aerodynamic diameter and respiratory deposition of fungal spores

Reponen, Tiina; Willeke, Klaus; Ulevičius, Vidmantas; Reponen, Auvo; Grinshpun, Sergey A.

doi:10.1016/1352-2310(96)00128-8

Cited by 151 publications

(96 citation statements)

References 18 publications

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“…Although sampling issues due to particle size effects are not unique to biological aerosol sampling, PBAP size and mass are often hard to predict based on complicated relationships between the biological tissue and environmental variables such as temperature and relative humidity (e.g. Madelin and Johnson, 1992;Reponen et al, 1996). In addition, many biological particles (e.g.…”

Section: Pbap Sampling Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Primary biological aerosol particles in the atmosphere: a review

Després¹,

Huffman

Burrows³

et al. 2012

Tellus B: Chemical and Physical Meteorology

1,105

1,103

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A B S T R A C T Atmospheric aerosol particles of biological origin are a very diverse group of biological materials and structures, including microorganisms, dispersal units, fragments and excretions of biological organisms. In recent years, the impact of biological aerosol particles on atmospheric processes has been studied with increasing intensity, and a wealth of new information and insights has been gained. This review outlines the current knowledge on major categories of primary biological aerosol particles (PBAP): bacteria and archaea, fungal spores and fragments, pollen, viruses, algae and cyanobacteria, biological crusts and lichens and others like plant or animal fragments and detritus. We give an overview of sampling methods and physical, chemical and biological techniques for PBAP analysis (cultivation, microscopy, DNA/RNA analysis, chemical tracers, optical and mass spectrometry, etc.). Moreover, we address and summarise the current understanding and open questions concerning the influence of PBAP on the atmosphere and climate, i.e. their optical properties and their ability to act as ice nuclei (IN) or cloud condensation nuclei (CCN). We suggest that the following research activities should be pursued in future studies of atmospheric biological aerosol particles: (1) develop efficient and reliable analytical techniques for the identification and quantification of PBAP; (2) apply advanced and standardised techniques to determine the abundance and diversity of PBAP and their seasonal variation at regional and global scales (atmospheric biogeography); (3) determine the emission rates, optical properties, IN and CCN activity of PBAP in field measurements and laboratory experiments; (4) use field and laboratory data to constrain numerical models of atmospheric transport, transformation and climate effects of PBAP.

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Section: Pbap Sampling Methodsmentioning

confidence: 99%

“…Laboratory studies of ice nucleation active biological particles. Since the first ice-nucleating biological particles were discovered, numerous microorganisms have Reponen et al (1996) Wex et al (2010) been screened for ice nucleation activity. A selection of results is summarised in Table 6.…”

Section: Pbap As Ice Nucleimentioning

confidence: 99%

Primary biological aerosol particles in the atmosphere: a review

Després¹,

Huffman

Burrows³

et al. 2012

Tellus B: Chemical and Physical Meteorology

1,105

1,103

View full text Add to dashboard Cite

show abstract

“…In addition, the size of fungal spores has been found to depend on the relative humidity, i.e. higher the relative humidity is, the larger the spores are (Reponen et al, 1996;Madsen, 2012). The spores of basidiomycete fungi are in general larger than those of ascomycete (Reponen et al, 2001;Manninen et al, 2014;Hussein et al, 2013;, and the basidiomycete fungi are known to sporulate mainly during autumn when the relative humidity is high (Kauserud et al, 2011).…”

Section: Overview Of Meteorological Factors and Sourcesmentioning

confidence: 99%

Characterization of free amino acids, bacteria and fungi in size-segregated atmospheric aerosols in boreal forest: seasonal patterns, abundances and size distributions

et al. 2017

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Abstract. Primary biological aerosol particles (PBAPs) are ubiquitous in the atmosphere and constitute ∼ 30 % of atmospheric aerosol particle mass in sizes > 1 µm. PBAP components, such as bacteria, fungi and pollen, may affect the climate by acting as cloud-active particles, thus having an effect on cloud and precipitation formation processes. In this study, size-segregated aerosol samples (< 1.0, 1-2.5, 2.5-10 and > 10 µm) were collected in boreal forest (Hyytiälä, Finland) during a 9-month period covering all seasons and analysed for free amino acids (FAAs), DNA concentration and microorganism (bacteria, Pseudomonas and fungi). Measurements were performed using tandem mass spectrometry, spectrophotometry and qPCR, respectively. Meteorological parameters and statistical analysis were used to study their atmospheric implication for results. Distinct annual patterns of PBAP components were observed, late spring and autumn being seasons of dominant occurrence. Elevated abundances of FAAs and bacteria were observed during the local pollen season, whereas fungi were observed at the highest level during autumn. Meteorological parameters such as air and soil temperature, radiation and rainfall were observed to possess a close relationship with PBAP abundances on an annual scale.

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“…Previous culture-based methods for environmental airborne fungi and time-of-flight based methods applied to pure culture (Madelin and Johnson, 1992;McCartney et al, 1993;Reponen et al, 1996) may underestimate fungal quantities in larger particles as they are incapable of quantifying multiple cells in an aggregate (Heikkila et al, 1988).…”

Section: Abundance Of Allergenic and Pathogenic Fungimentioning

confidence: 99%

Particle-size distributions and seasonal diversity of allergenic and pathogenic fungi in outdoor air

et al. 2012

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Fungi are ubiquitous in outdoor air, and their concentration, aerodynamic diameters and taxonomic composition have potentially important implications for human health. Although exposure to fungal allergens is considered a strong risk factor for asthma prevalence and severity, limitations in tracking fungal diversity in air have thus far prevented a clear understanding of their human pathogenic properties. This study used a cascade impactor for sampling, and quantitative real-time PCR plus 454 pyrosequencing for analysis to investigate seasonal, size-resolved fungal communities in outdoor air in an urban setting in the northeastern United States. From the 20 libraries produced with an average of ∼800 internal transcribed spacer (ITS) sequences (total 15 326 reads), 12 864 and 11 280 sequences were determined to the genus and species levels, respectively, and 558 different genera and 1172 different species were identified, including allergens and infectious pathogens. These analyses revealed strong relationships between fungal aerodynamic diameters and features of taxonomic compositions. The relative abundance of airborne allergenic fungi ranged from 2.8% to 10.7% of total airborne fungal taxa, peaked in the fall, and increased with increasing aerodynamic diameter. Fungi that can cause invasive fungal infections peaked in the spring, comprised 0.1–1.6% of fungal taxa and typically increased in relative abundance with decreasing aerodynamic diameter. Atmospheric fungal ecology is a strong function of aerodynamic diameter, whereby through physical processes, the size influences the diversity of airborne fungi that deposit in human airways and the efficiencies with which specific groups of fungi partition from outdoor air to indoor environments.

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Effect of relative humidity on the aerodynamic diameter and respiratory deposition of fungal spores

Cited by 151 publications

References 18 publications

Primary biological aerosol particles in the atmosphere: a review

Primary biological aerosol particles in the atmosphere: a review

Characterization of free amino acids, bacteria and fungi in size-segregated atmospheric aerosols in boreal forest: seasonal patterns, abundances and size distributions

Particle-size distributions and seasonal diversity of allergenic and pathogenic fungi in outdoor air

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