2017
DOI: 10.5194/acp-17-13089-2017
|View full text |Cite
|
Sign up to set email alerts
|

Characterization of free amino acids, bacteria and fungi in size-segregated atmospheric aerosols in boreal forest: seasonal patterns, abundances and size distributions

Abstract: Abstract. Primary biological aerosol particles (PBAPs) are ubiquitous in the atmosphere and constitute ∼ 30 % of atmospheric aerosol particle mass in sizes > 1 µm. PBAP components, such as bacteria, fungi and pollen, may affect the climate by acting as cloud-active particles, thus having an effect on cloud and precipitation formation processes. In this study, size-segregated aerosol samples (< 1.0, 1-2.5, 2.5-10 and > 10 µm) were collected in boreal forest (Hyytiälä, Finland) during a 9-month period covering a… Show more

Help me understand this report
View preprint versions

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

4
54
1

Year Published

2018
2018
2022
2022

Publication Types

Select...
5
1

Relationship

1
5

Authors

Journals

citations
Cited by 46 publications
(59 citation statements)
references
References 74 publications
4
54
1
Order By: Relevance
“…Compilations of speciated cloud water organics have shown that small acids, such as formic and acetic acids, comprise a large fraction (at least ∼ 30 %) of the characterized fraction of cloud water organics (e.g., Fig. 6 in Herckes et al, 2013). The calculated rates, R Bact,WSOC , are summarized in Fig.…”
Section: Calculation Of Microbial and Chemical Wsoc Loss Ratesmentioning
confidence: 97%
See 3 more Smart Citations
“…Compilations of speciated cloud water organics have shown that small acids, such as formic and acetic acids, comprise a large fraction (at least ∼ 30 %) of the characterized fraction of cloud water organics (e.g., Fig. 6 in Herckes et al, 2013). The calculated rates, R Bact,WSOC , are summarized in Fig.…”
Section: Calculation Of Microbial and Chemical Wsoc Loss Ratesmentioning
confidence: 97%
“…In turn, in the same study, it was shown that when substrate availability is limited, values from ∼ 7 % to ∼ 14 % are generally observed. As the conditions in cloud water can be considered oligotrophic with typical concentrations of dissolved organic carbon (DOC) of less than 0.1 mM (Herckes et al, 2013), low BGEs in the range of 0.1 %-10 % can be expected, i.e., DOC is efficiently converted into CO 2 . Bacterial cells are composed not only of carbon but also other elements such as nitrogen and phosphorus, and the proportions of which can vary widely depending on nutrient condition (e.g., Vrede et al, 2002;Chrzanowski and Kyle, 1996).…”
Section: Bacterial Growth Efficiency (Bge)mentioning
confidence: 99%
See 2 more Smart Citations
“…Fungal 18S ribosomal RNA (rRNA) and bacterial 16S rRNA sequences were determined by quantitative PCR (qPCR) using target-specific primer pairs: FF390 (5 0 -ATTACCGCGGCTGCTGG-3 0 ) and FR1 (5 0 -AIC-CATTCAATCGGTAIT-3 0 ) (Vainio and Hantula 2000) for fungi, and Eub338F (5 0 -ACTCCTACGG-GAGGCAGCAG-3 0 ) and Eub518R (5 0 ATTACC GCGGCTGCTGG-3 0 ) (Fierer and Jackson 2005) for bacteria. qPCR was carried out using a Bio-Rad CFX96 iCycler on 96-well white-welled polypropylene plates (Bio-Rad) as previously described (Helin et al 2017). Briefly, the reaction mixture contained a 19 SsoAdvanced universal SYBR Green Supermix (Bio-Rad, USA), 0.3-0.6 ng of template DNA, 250 nM of Eub338F and Eub518R primers for bacteria, or 3-6 ng of template DNA, 250 nM FF390 and 200 nM FR1 primers for fungi with the reaction volume set to 20 ll using nuclease-free water.…”
Section: Dna Extraction and Quantitative Pcrmentioning
confidence: 99%