Effect of regeneration procedures on the genetic integrity of Brassica oleracea accessions

Soengas, Pilar; Cartea, María Elena; Márquez, Margarita Lema; Velasco, Pablo

doi:10.1007/s11032-008-9241-z

Cited by 17 publications

(13 citation statements)

References 26 publications

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“…When Börner et al (2000) studied, with the help of microsatellite markers, the genetic identity of wheat accessions regenerated up to 24-times, genetic drift was observed in one out of eight wheat accession. Changes in allele frequencies due to the action of genetic drift, directional selection and assortative mating were detected also by Le Clerc et al (2003), van Hintum et al (2007) and Soengas et al (2009), who studied the effect of regeneration procedures on the integrity of several opencrossing species. Recently, the study of open-crossing Brassica oleracea L. landraces showed significant changes in the allele frequency of individual loci even after only two regeneration cycles (Soengas et al 2009).…”

Section: Discussionmentioning

confidence: 65%

“…Changes in allele frequencies due to the action of genetic drift, directional selection and assortative mating were detected also by Le Clerc et al (2003), van Hintum et al (2007) and Soengas et al (2009), who studied the effect of regeneration procedures on the integrity of several opencrossing species. Recently, the study of open-crossing Brassica oleracea L. landraces showed significant changes in the allele frequency of individual loci even after only two regeneration cycles (Soengas et al 2009). Similarly to our data, Parzies et al (2000) observed significant decline in the average gene diversity and allele number per locus over the time of barley landraces conserved for different time.…”

Section: Discussionmentioning

confidence: 65%

“…By analysis of temporally differing samples, the existence and importance of genetic drift was determined by morphological, biochemical and DNA markers (Chebotar et al 2002;Chwedorzewska et al 2002;Enjabert et al 1999;Parzies et al 2000). Besides genetic drift affecting all polymorphic loci, selection was reported affecting traits for which genetic variability was linked to different levels of survival and reproduction (Breese 1989;Le Clerc et al 2003;Soengas et al 2009). This is due to the conditions of regeneration, which are different from those in which original samples were collected (usually being more uniform in culture) leading to a selection advantage of certain genotypes and gradual reduction of genetic diversity for any given accession.…”

Section: Introductionmentioning

confidence: 99%

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Molecular evidence of genetic diversity changes in pea (Pisum sativum L.) germplasm after long-term maintenance

Cieslarová

Smýkal

Dočkalová

et al. 2010

Genet Resour Crop Evol

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Maintaining germplasm genetic integrity is a key objective of long-term ex situ conservation. Periodic regeneration, performed on limited plots with small number of individuals, increases the risk of genetic drift and genetic diversity changes. In this study, six accessions of white flowered, dry seed pea varieties (Pisum sativum L. subsp. sativum var. sativum): Bohatýr, Klatovský zelený, Hanák, Moravský hrotovický krajový, Raman and Viktoria-75 and four accessions of colour flowered, fodder pea (P. sativum subsp. sativum var. arvense (L.) Poiret: Arvika, Č eský banán, Moravská krajová and Niké, representing Czechoslovak varieties and landraces, bred over the last 40-80 years, were analyzed using ten microsatellite locus specific markers. Each accession was represented by 20 individual seeds of two temporally different samples, spanning the period of 20 or 40 years. Together with intra-accession variation (except of cv. Hanák), evidence of genetic changes, e.g. differences in allele frequencies as well as genetic composition of sample, was detected in six out of ten accessions (Arvika, Bohatýr, Č eský banán, Moravský hrotovický krajový, Moravská krajová and Raman). Evidence of genetic erosion was found in three accessions (Č eský banán, Moravský hrotovický krajový and Raman), while in another three (Arvika, Bohatýr and Moravská krajová) the level of diversity was found to have increased. Moreover in three samples of Bohatýr (2004) and Klatovský zelený (1963 and2004), low levels of heterozygosity was detected. These results demonstrate that in pea, a selfpollinating and highly homozygous plant, the danger of the loss of genetic integrity exists. These findings are significant for long-term ex situ germplasm management.

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 65%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular evidence of genetic diversity changes in pea (Pisum sativum L.) germplasm after long-term maintenance

Cieslarová

Smýkal

Dočkalová

et al. 2010

Genet Resour Crop Evol

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“…Only a few investigation of the genetic integrity have been performed on long-term conserved accessions undergoing periodical regeneration and changes in allelic frequencies were detected in various species (Enjalbert et al 1999;Parzies et al 2000;Le Clerc et al 2003;van Hintum et al 2007;Smýkal et al 2008;Soengas et al 2009;Cieslarová et al 2011). These findings imply that regeneration protocols should be improved to accommodate more numerous samples (larger population, Ne) and the composition of the collection should be continuously monitored to prevent the risk of genetic diversity loss.…”

Section: Developing Global Conservation Strategiesmentioning

confidence: 99%

Legume genetic resources: management, diversity assessment, and utilization in crop improvement

et al. 2011

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Grain legumes contribute significantly to total world food production. Legumes are the primary source of dietary proteins in many developing countries, where protein hunger and malnutrition are widespread. Grain legumes germplasm constitute ~15% of the 7.4 M accessions preserved globally. Nearly, 78% of the CGIAR?s, 0.217 M accessions, have been characterized, compared to 34% of national genebank collections. Interestingly, limited data on grain quality are available as the primary focus has been on morpho-agronomic traits. Clearly, more resources should be targeted on biochemical evaluation to identify nutritionally rich and genetically diverse germplasm. The formation of core and mini core collections has provided crop breeders with a systematic yet manageable entry point into global germplasm resources. These subsets have been reported for most legumes and have proved useful in identifying new sources of variation. They may however not eliminate the need to evaluate entire collections, particularly for very rare traits. Molecular characterization and association mapping will further aid to insights into the structure of legume diversity and facilitate greater use of collections. The use of high resolution elevational climate models has greatly improved our capacity to characterize plant habitats and species? adaptive responses to stresses. Evidence suggests that there has been increased use of wild relatives as well as new resources resulting from mutagenesis to enhance the genetic base of legume cultigens.Peer reviewe

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“…Much eff ort has been put into establishing ex situ collections representing landrace and wild relative diversity of cultivated crop species. Regardless of the level of intra-accession diversity, there have been relatively few empirical studies that directly examine the impact of ex situ conservation procedures on genetic variation (Parzies et al, 2000;Börner et al, 2000;Chebotar et al, 2003;Van Hintum et al, 2007;Soengas et al, 2009;Hirano et al, 2009). Trade-off s typically can be viewed as a logistic limitation (increasing regeneration sizes) vs. a quality limitation (loss of genetic diversity).…”

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confidence: 99%

Modeling Demographics and Genetic Diversity in Ex Situ Collections during Seed Storage and Regeneration

Richards¹,

Lockwood

Volk³

et al. 2010

Crop Science

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Gene banks have a critical role in preserving genetic diversity for many conservation uses. A key challenge in this task is maintaining both the genetic integrity and the viability of a collection during regeneration and long‐term storage. In this study we examine the interacting effects of sampling and viability decline from seed aging on genetic diversity. To do this, we developed a model that simulates sampling during regeneration of individual genotypes using two commonly used but contrasting regeneration protocols. Critically, the model simulates a realistic nonlinear decline in viability across time, and the combined effects of sampling and mortality were evaluated for their effect on allelic diversity. In addition, the model was used to contrast the kinetics of allelic richness among rounds of regeneration in accessions with different heterozygosity levels (e.g., cultivars vs. wild) and different breeding systems (outcrossing vs. inbred). Results underscore a significant interaction between regeneration timing and regeneration size. Regeneration procedures should consider viability kinetics and intra‐accession diversity to best manage genetic erosion. These changes highlight the trade‐off faced by curators between the logistics of increasing regeneration sampling size and improving the technical procedures for seed storage and monitoring.

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Effect of regeneration procedures on the genetic integrity of Brassica oleracea accessions

Cited by 17 publications

References 26 publications

Molecular evidence of genetic diversity changes in pea (Pisum sativum L.) germplasm after long-term maintenance

Molecular evidence of genetic diversity changes in pea (Pisum sativum L.) germplasm after long-term maintenance

Legume genetic resources: management, diversity assessment, and utilization in crop improvement

Modeling Demographics and Genetic Diversity in Ex Situ Collections during Seed Storage and Regeneration

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