Molecular evidence of genetic diversity changes in pea (Pisum sativum L.) germplasm after long-term maintenance

Cieslarová, Jaroslava; Smýkal, Petr; Dočkalová, Zuzana; Hanáček, Pavel; Procházka, Stanislav; Hýbl, Miroslav; Griga, Miroslav

doi:10.1007/s10722-010-9591-3

Cited by 28 publications

(22 citation statements)

References 33 publications

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“…In addition, they were obtained from genebanks where they have certainly undergone several cycles of regeneration. Depletion of genetic diversity throughout successive regenerations of accessions has been demonstrated elsewhere (Cieslarová et al, 2011). Genetic diversity indices (Ho and He) and fixation index (F) estimated in this study were close to those reported from several previous studies based on SSR markers (Nantoumé et al, 2013;Gbotto et al, 2015) and were generally low.…”

Section: Discussionsupporting

confidence: 90%

Microsatellite-based genetic diversity among accessions of Citrullus spp. collected from 36 countries

Angui¹,

Koffi²,

Koffi³

et al. 2017

Aust J Crop Sci

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Watermelon (Citrullus lanatus) is one of the most economically important vegetable cucurbits. However, the genetic and taxonomic statuses of its closely related species remain scantly documented, limiting their full use for agronomic purposes. The genetic diversity and structure of 74 accessions covering 47 dessert type (C. lanatus subsp. vulgaris), 21 oilseed type (C. mucosospermus), and 6 citron melon (C. lanatus subsp. lanatus var citroides) collected from 36 countries throughout 4 continents, were analyzed using 18 polymorphic SSR markers. The mean values of proportion of polymorphic loci (P = 29.73), number of alleles per locus (A = 1.243), effective number of alleles per locus (Ae = 1.153) Shannon index (I = 0.191), observed and expected heterozygosities (Ho = 0.124; He = 0.149) confirmed the narrow genetic basis of C. lanatus. According to molecular variance analysis the most important component of the genetic variation was obtained among accessions (70%). On the contrary, lower genetic variation was noted among species (16%), countries (37%), and continents (14%). It is suggested that the cultivated forms of Citrullus spp. originated from or successive selection cycles aimed at few and/or common traits, in few ancestral populations. Clustering based on both Bayesian approach and an unweighted pair group method with arithmetic mean pointed out three groups of accessions corresponding to use types and collecting countries. Based on these results, future collecting missions could be focused mainly on representative ecological sites in Citrullus spp. distribution areas, and increasing the number of accessions and seeds per accession.

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Section: Discussionsupporting

confidence: 90%

Microsatellite-based genetic diversity among accessions of Citrullus spp. collected from 36 countries

Angui¹,

Koffi²,

Koffi³

et al. 2017

Aust J Crop Sci

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“…This study investigated changes in the genetic diversity of pea genetic resources over the period of 70 years by the analysis of microsatellite (SSR) and retrotransposon (RBIP) markers, while in our previous study we examined the genetic erosion in ex situ collections, as a result of regeneration and storage practices (Cieslarová et al 2011a). We focused on pea genetic resources, where there is a limited possibility of in situ conservation and thus only ex situ germplasm collections are the essential reservoir for pea genetic diversity.…”

Section: Discussionmentioning

confidence: 99%

“…Previously we investigated changes in the genetic integrity of pea collection in the process of germplasm maintenance (Cieslarová et al 2011a) and reported an evidence of genetic erosion. This study focuses on the temporal diversity changes in the Czech/Czechoslovak pea germplasm collection as assessed by microsatellite and retrotransposon markers in three time periods spanning the 70 years of pea breeding.…”

mentioning

confidence: 99%

Molecular analysis of temporal genetic structuring in pea (Pisum sativum L.) cultivars bred in the Czech Republic and in former Czechoslovakia since the mid-20th century

Cieslarová¹,

Hýbl²,

Griga³

et al. 2012

Czech J. Genet. Plant Breed.

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Changes in genetic diversity of peas bred in the Czech Republic and in former Czechoslovakia since the mid-20 th century were analysed using 38 molecular marker loci, including retrotransposons and microsatellites, differentiating a total of 84 alleles. Both marker types were comparably effective in revealing the genetic diversity, with a high correlation (r = 0.81), although the pairwise genetic distances of each marker type differed. In total, 175 accessions, selected from the Czech pea gene bank collection and representing the pea cultivars collected or bred in the country, were divided into three groups according to their date of sampling or variety registration. The first group contained 70 old cultivars and landraces collected prior to 1961. The second group contained 46 cultivars released from 1961 to 1980. The third group contained 59 cultivars released between 1981 and 2004. In spite of the decline in several diversity measures, differences in allele frequencies and even allele loss in three microsatellite loci were recorded over the 70-year period, while these differences between the groups were not statistically significant. In addition, genetic heterogeneity was detected in 29 accessions (15%). This indicates that although no genetic erosion could be observed since then, it is important to monitor the genetic diversity, furthermore it highlights the vital role of germplasm collections for the crop diversity conservation.

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“…However, genebank accessions collected across several decades showed no consistent loss of genetic diversity, and we found both high-and low-level genetic diversity in different accessions collected at the same time. In contrast, loss of genetic diversity in pea accessions during genebank maintenance has previously been reported in a study of Czechoslovak pea landraces and cultivars (Cieslarová et al, 2010). The low-diversity NordGen accessions can be because of either low diversity of the source populations or the sampling procedures during the genebank donation.…”

Section: Discussionmentioning

confidence: 88%

Twentieth-century changes in the genetic composition of Swedish field pea metapopulations

Leino¹,

Boström

Hagenblad

2012

Heredity

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Landrace crops are formed by local adaptation, genetic drift and gene flow through seed exchange. In reverse, the study of genetic structure between landrace populations can reveal the effects of these forces over time. We present here the analysis of genetic diversity in 40 Swedish field pea (Pisum sativum L.) populations, either available as historical seed samples from the late nineteenth century or as extant gene bank accessions assembled in the late twentieth century. The historical material shows constant high levels of within-population diversity, whereas the extant accessions show varying, and overall lower, levels of within-population diversity. Structure and principal component analysis cluster most accessions, both extant and historical, in groups after geographical origin. County-wise analyses of the accessions show that the genetic diversity of the historical accessions is largely overlapping. In contrast, most extant accessions show signs of genetic drift. They harbor a subset of the alleles found in the historical accessions and are more differentiated from each other. These results reflect how, historically present metapopulations have been preserved during the twentieth century, although as genetically isolated populations.

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Molecular evidence of genetic diversity changes in pea (Pisum sativum L.) germplasm after long-term maintenance

Cited by 28 publications

References 33 publications

Microsatellite-based genetic diversity among accessions of Citrullus spp. collected from 36 countries

Microsatellite-based genetic diversity among accessions of Citrullus spp. collected from 36 countries

Molecular analysis of temporal genetic structuring in pea (Pisum sativum L.) cultivars bred in the Czech Republic and in former Czechoslovakia since the mid-20th century

Twentieth-century changes in the genetic composition of Swedish field pea metapopulations

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