Providing low-protein diets, that are supplemented with free amino acids to cover the amino acid requirement of pigs, might be helpful in reducing the use of soybean meal of Latin American origin, and thus in reducing the amount of non-EU protein in the diet. In pig farming, liquid feeding is frequently applied. Research from Denmark demonstrated that free amino acids can be highly sensitive for degradation during fermentation of liquid feed. The aim of the current study was to determine the degradation of free amino acids in freshly produced liquid feeds under very mild fermentation conditions. Two liquid feeds, without and with free amino acids, were compared. On a dry matter basis, feed consisted of 50%, 30%, 10%, and 10% complement diet, liquid wheat starch, potato steam peels, and wheat yeast concentrate, respectively. Free lysine, methionine, threonine, and tryptophan were supplemented to the complement feed with free amino acids. After mixing the liquid feeds, samples of the liquid feed were analysed for pH and amino acid contents after 0, 1, 2, 4 and 8h of incubation. Samples were treated to receive separate fractions with insoluble protein, plant protein, bacterial protein and free amino acids, where amino acid contents were determined in all fractions at each sampling time. The most important conclusions of this study are summarised below. The analysed amino acid contents of the liquid coproducts and the complement feeds were in agreement with the calculated table values. The analysed contribution of the free amino acids to the liquid feed was slightly lower than calculated. The sum of analysed amino acids in the distinguished fractions insoluble protein, plant protein, bacterial protein and free amino acids was considerably lower than expected based on the analysed amino acid contents of the liquid coproducts and the complement feeds. The pH was 4.2 at the start of the incubation and increased to 4.5-4.6 after 8h of incubation, while pH level was not affected by the free amino acid concentration. The liquid feed at the start of the incubation period had a low microbial load and a high concentration of lactic acid. The amounts of free Lysine, methionine and threonine that were degraded over the 8h incubation period were estimated to be 9%, 3%, and 0-30%, respectively. Compared to findings in Danish studies, a relatively low level of degradation of free lysine, methionine, and threonine was observed. These low levels of degradation of free AA might be explained by the limited availability of Enterobacteriaceae, as well as by the high concentration of lactic acid at the start of the incubation period. The pH level of the liquid feed remained below 4.6 during the 8h incubation period, indicating no large shift in the presence of volatile fatty acids, while this low pH hampers the development of coliforms, which are able to degrade free amino acids for the production of biogenic amines. It can be concluded that the level of degradation of free amino acids in liquid feeds might be limited, provided...