Effect of reduced temperatures and brefeldin A on prolactin secretion and on subcellular distribution of the secretory product and membrane antigens in GH3 pituitary cells*

Nasciutti, Luiz-Eurico; Picart, R; Rosenbaum, Etelka; Tixier-Vidal, A; Tougard, Claude

doi:10.1016/0248-4900(92)90121-g

Cited by 5 publications

(3 citation statements)

References 25 publications

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“…In vitro PRL secretion from GH3 cells was suppressed at low culture temperatures (10°-20°C) [60]. Both basal production and secretion of PRL in the pituitary cells from the pig reared at 2JD-34°C were higher than those from the pig reared at 20°C.…”

Section: Prolactinmentioning

confidence: 94%

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Endocrine Responses to Heat and Cold Stress

Matsumoto

Nishiyama

Nishimura

et al. 2001

Thermotherapy for Neoplasia, Inflammation, and Pain

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Summary. This review focuses on the endocrine responses to thermal stimuli during passive heat or cold exposure, with particular reference to the relation of these responses to the changes in the body core temperature (Teare). Mild to moderate hyperthermia (<1°C rise in Teare) induces the release of growth hormone and prolactin (PRL). Moderate hypothermia (la_2°C fall in Teore) suppresses PRL release. A positive correlation between plasma PRL and Teare suggests some role for PRL in thermoregulation. Hypothermia activates the hypothalamo-pituitary-thyroid (HPT) axis and releases thyrotropin-releasing hormone, thyroid-stimulating hormone (TSH), and thyroid hormones and increases the metabolic rate. Enhancement of extrathyroidal production of triiodothyronine (T3) from thyroxine (T4) may precede the TSH response to cold. Both severe hyperthermia and hypothermia (1°_3°C changes in Teare) activate the hypothalamo-pituitary-adrenal (HPA) axis and the sympathetic nervous system, resulting in release of corticotropin-releasing factor, adrenocorticotropic hormone, cortisol, and norepinephrine. The responses in the HPT axis and the HPA axis are not apparent in humans, as they are in rats, probably owing to the larger body mass of humans. Hyperthermia stimulates the renin-angiotensin-aldosterone system and the release of arginine vasopressin (AVP) and atrial natriuretic peptide, but this might be due to nonthermal factors. Diuresis due to suppression of AVP release is induced by cold. Gonadal response to thermal stimuli is possibly suppressive. The hormonal responses induced by thermal stress are mostly dependent on the change in Teare in humans; in small animals they are also dependent on the change in skin temperature. I ntrod uctionBody core temperature (Teare) may increase during physical exercise and passive heating (while sauna bathing, with head-out water immersion, and exposure to hot environments); it decreases during cold exposure. Changes in Teare induce various endocrine responses to maintain homeostasis of the body. Recently, local and whole-body hyperthermia therapy for cancer and some benign diseases and induced hypothermia treatment to protect the important organs from hypoxic damage have been developed and widely utilized. On these occasions, the endocrine responses induced by the changes in Teare should be considered. Hormonal responses and their relation to the immune system during exercise in the heat and passive hyperthermia have been reviewed [1][2][3], but little attention has been paid to the relation of endocrine responses to the change in Teare. Body heating and cooling paradigms may include concurrent psychological stress. This review focuses on the endocrine responses to thermal stimuli during passive heating and cooling, with particular reference to their relation to changes in Teare. The study was undertaken to elucidate the thermal influence alone, excluding the influence of non thermal stress. Growth HormoneThere have been many consistent findings that hyperthermia induces a rapid rise in ci...

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Section: Prolactinmentioning

confidence: 94%

“…There is some in vitro evidence that temperature directly influences PRL secretion from the pituitary cells [60,61). In vitro PRL secretion from GH3 cells was suppressed at low culture temperatures (10°-20°C) [60].…”

Section: Prolactinmentioning

confidence: 98%

Endocrine Responses to Heat and Cold Stress

Matsumoto

Nishiyama

Nishimura

et al. 2001

Thermotherapy for Neoplasia, Inflammation, and Pain

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“…We have already described the effect of these treatments on the distribution and secretion of prolactin in GH3B6 cells (30). BFA is a fungal metabolite that blocks anterograde transport of proteins at different steps of the secretory pathway (reviewed in Ref.…”

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confidence: 99%

Proteolytic Processing of Sulfated Secretogranin II in the trans-Golgi Network of GH3B6 Prolactin Cells

Muller

Barret

Picart

et al. 1997

Journal of Biological Chemistry

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Secretogranin II (SgII) is a protein specific to the matrix of the secretory granules in neurons and neuroendocrine cells. We have already demonstrated the precursor-product relationship between sulfated SgII and four N-terminal derived peptides in GH3B6 prolactin cells. In this study, we have investigated the subcellular compartment in which the cleavage of SgII is initiated by taking advantage of its tyrosine sulfation in the transGolgi network (TGN). In order to prevent export of radiosulfated SgII from the TGN, we used brefeldin A (BFA) as well as incubation at 20°C. BFA completely inhibited the cleavage of SgII when added immediately post-pulse. BFA added a few minutes post-pulse or after a 20°C incubation, however, permitted the cleavage of SgII in the presence of the drug. These SgII-derived peptides generated in the presence of BFA could not be released upon stimulation of the cells by either thyroliberin, a physiological secretagogue, or KCl. These results demonstrate that SgII can be cleaved in the TGN. They also evidence that the cleavage occurs in a distal compartment of the TGN different from the sulfation site. The transfer of SgII from the sulfation site to this distal compartment of the TGN involves BFA-sensitive membrane dynamics.Bioactive peptides and hormones secreted by neurons and neuroendocrine cells are synthesized as precursors that must undergo post-translational processing before their release. The different steps of this processing occur during the vectorial transport of the proteins from the rough endoplasmic reticulum, where they are synthesized, to the secretory granules, in which they are stored. Proteolytic processing of the precursors is one of the last of these post-translational modifications. Although it occurs mainly in the secretory granules, it can be initiated in the trans-Golgi network (TGN) 1 (reviewed in Ref. 1). Indeed, immunocytochemical (2-5) and biochemical studies (6 -9) have evidenced the cleavage of precursors in the TGN as well as in the secretory granules. In this study, we have investigated the intracellular cleavage site of secretogranin II (SgII) in GH3B6 prolactin cells.SgII is a member of the granin family (reviewed in Ref. 10).These proteins are specific to the dense core secretory granules of neuroendocrine cells and neurons (reviewed in Ref. 35 S]sulfate has already allowed the use of sulfated SgII as a marker of intracellular transport between the TGN and the plasma membrane via the dense core secretory granules (23-25). PC12 cells, however, lack the prohormone convertases PC1 and PC2, which are involved in the processing of SgII (26,27). Our experiments were performed in GH3B6 cells, a subclone of GH3 cells, which express PC2 but not PC1 (28). Cultivating these cells in the presence of insulin, 17␤-estradiol, and epidermal growth factor increases the number of secretory granules (29), the expression of PC2 (28) and the storage of SgII-derived peptides in the granules (19). GH3B6 cells therefore provide an ideal model for studying the proteolytic proce...

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The Intermediate Compartment between Endoplasmic Reticulum and Golgi Complex in Mammalian Cells

Bonatti

Torrisi

1993

Subcellular Biochemistry

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Effect of reduced temperatures and brefeldin A on prolactin secretion and on subcellular distribution of the secretory product and membrane antigens in GH3 pituitary cells*

Cited by 5 publications

References 25 publications

Endocrine Responses to Heat and Cold Stress

Endocrine Responses to Heat and Cold Stress

Proteolytic Processing of Sulfated Secretogranin II in the trans-Golgi Network of GH3B6 Prolactin Cells

The Intermediate Compartment between Endoplasmic Reticulum and Golgi Complex in Mammalian Cells

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