Effect of Recombinant Human Bone Morphogenetic Protein-2 and Adipose Tissue-Derived Stem Cell on New Bone Formation in High-Speed Distraction Osteogenesis

Lee, Sung Joo; Kim, Byung Jun; Kim, Young Il; Sohn, Chul‐Ho; Jeon, Yoon Kyung; Xu, Lei; Kim, Sang Hyon; Kwon, Sun Young; Choi, Tae Hyun; Kim, Suk Wha

doi:10.1597/12-290

Cited by 9 publications

(9 citation statements)

References 24 publications

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“… 15 , 49 The number of transplanted cells ranged from 1 to 30×10 6 . The cell injection timing included three different time points: in three studies, MSCs were injected during the distraction phase 15 , 37 , 49 or during the consolidation phase; 30 , 38 , 43 , 44 , 45 , 47 , 48 , 51 in three studies, the MSCs were injected on the day of the operation; 16 , 20 , 50 and in one study the time of injection was not stated 52 . The majority of the studies reported that MSC transplantation at the end of the lengthening phase with MSCs over 1 x 10 6 enhanced bone consolidation or formation in DO in animal models.…”

Section: Resultsmentioning

confidence: 99%

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Stem cell therapy for enhancement of bone consolidation in distraction osteogenesis

Yang

Lin

Wang

et al. 2017

Bone & Joint Research

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ObjectivesDistraction osteogenesis (DO) mobilises bone regenerative potential and avoids the complications of other treatments such as bone graft. The major disadvantage of DO is the length of time required for bone consolidation. Mesenchymal stem cells (MSCs) have been used to promote bone formation with some good results.MethodsWe hereby review the published literature on the use of MSCs in promoting bone consolidation during DO.ResultsStudies differed in animal type (mice, rabbit, dog, sheep), bone type (femur, tibia, skull), DO protocols and cell transplantation methods.ConclusionThe majority of studies reported that the transplantation of MSCs enhanced bone consolidation or formation in DO. Many questions relating to animal model, DO protocol and cell transplantation regime remain to be further investigated. Clinical trials are needed to test and confirm these findings from animal studies.Cite this article: Y. Yang, S. Lin, B. Wang, W. Gu, G. Li. Stem cell therapy for enhancement of bone consolidation in distraction osteogenesis: A contemporary review of experimental studies. Bone Joint Res 2017;6:385–390. DOI: 10.1302/2046-3758.66.BJR-2017-0023.

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Section: Resultsmentioning

confidence: 99%

“…Dehghan et al, 36 Lee et al, 51 and Kinoshita et al 37 used the higher rate of lengthening, and the MSC therapy significantly promoted new bone formation and shortened the consolidation period in their DO models.…”

Section: Discussionmentioning

confidence: 99%

Stem cell therapy for enhancement of bone consolidation in distraction osteogenesis

Yang

Lin

Wang

et al. 2017

Bone & Joint Research

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“…First, only one injection time point was evaluated. Most previous studies have implanted cells into the distraction gap during either the distraction [11,14,20] or consolidation [12,13,15,16,19,23] phases. It is possible that exogenous MSCs do not play a significant role so early in latency, and systemic administration would be effective during later phases.…”

Section: Discussionmentioning

confidence: 99%

“…A body of pre-clinical research over the past 20 years indicates that addition of cells enhances the amount and quality of new bone generated by DO. [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] These studies have used stem cells or osteogenically differentiated cells with success. This has been shown in both mandibular [12,13,[16][17][18][19]25,26] and long bone distraction [11,14,15,[20][21][22][23][24] and could theoretically decrease the consolidation time.…”

Section: Introductionmentioning

confidence: 99%

Systemically administered MSCs given 24hrs after osteotomy do not affect bone formation in rat distraction osteogenesis

Guevara

Toth

Kim

et al. 2018

Preprint

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Distraction osteogenesis is a unique postnatal bone formation employed by orthopaedic surgeons to treat many conditions, however, the overall time to external frame removal can be extensive. Any strategies that accelerate healing would improve patient care. Distraction osteogenesis research in the past decade has shown that direct stem cell implantation enhances new bone formation. Systemic implantation would be more clinically desirable. Systemically delivered stem cells have been shown to home to a mandibular distraction site; however, effects on bone formation have not been studied. Ten-week-old, male Sprague-Dawley rats underwent surgery to implant an external fixator-distractor and an osteotomy was performed. Twenty-four hours postoperatively, each rat received tail vein injections of either saline or 10^6 fluorescently labeled primary mesenchymal stem cells. Animals in the validation groups were euthanized two days after surgery and the femora processed for histology. Animals in the experimental groups were given five days of latency, then the femur was lengthened once daily for five days (0.75mm/day, 3.75mm total). Following four weeks of consolidation, the animals were euthanized and the femora were evaluated by microCT and histology to quantify new bone formation. Labeled stem cells were found at the distraction site in validation animals. However, there were no differences in any bone or soft tissue outcomes. Systemic stem cell administration 24 hours after surgery does not improve DO outcomes. It is possible that the animal model was not challenging enough to discriminate any augmentation provided by stem cells.

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“…In order to reduce complication and improve the bone healing, several regenerative techniques have been associated with DO protocols. Low level laser therapy has been showed to reduce the time for bone regeneration of the DO in rabbits, gene therapy like addition of BMPs on the lengthened callus are reported to be able to accelerate bone maturation with different levels of success and cost [13,28] .…”

Section: Discussionmentioning

confidence: 99%

Adipose Derived Stem Cell on the Regeneration of Irradiated Mandible of Adult Rabbit Submitted to Distraction Osteogenesis

Soares¹,

Segreto²,

Aloise³

et al. 2018

JPP

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Purpose: To evaluate the effect of adipose derived mesenchymal stem cell (ADSC) on the regeneration of irradiated mandible submitted to distraction osteogenesis on adult rabbits. Methods: Ten adult rabbits were selected and the dorsal fat was harvested for isolation, dissociation and culture of adipose derived adult mesenchymal stem cells. The mandibles were irradiated on a single dose of 20 Gy. After 30 days the animals underwent mandibular lengthening by distraction osteogenesis. After 10 mm of lengthening, the animals were distributed into two groups. Saline solution (1 mL) was injected the control group, and supplemented with ADSC, on the study group. After 4 weeks the mandibles were removed and evaluated by tomography and histomorphometric analysis. Histomorphometric was obtained and statistical analysis done by Mann-Withney test (p > 0.05). Results: The tomography shows a significant improvement on the Hounsfield scale for lateral fibrovascular zone (114.5 ± 9.7 control group to 148 ± 24, p = 0.0045) and central fibrovascular zone (37 ± 13.4 control group to 96 ± 41.8 on study group p = 0.0045). The Histomorphometric analysis demonstrated an increase area of new bone formation. The mineralized area was of 61.2 ± 9.9% in the study group and 31.3 ± 10.6% in the control group (p = 0.0045) for the central fibrovascular zone, 79.1 ± 9.1% in the study group and 39.1 ± 14.7 on control group for anterior fibrovascular zone (p = 0.0001) and 67.7 ± 12.9% in the study group and 38.4 ÷ 12.9% in control group for posterior fibrovascular zone (p = 0.0001). Conclusions: The injection of adipose derived mesenchymal stem cell was effective to improve bone regeneration distraction callus on the irradiated mandible.

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Effect of Recombinant Human Bone Morphogenetic Protein-2 and Adipose Tissue-Derived Stem Cell on New Bone Formation in High-Speed Distraction Osteogenesis

Abstract: This study suggests that the use of rhBMP-2 in combination with or without ADSC is helpful to promote bone regeneration in high-speed distraction osteogenesi s of adult rabbit cranium.

Cited by 9 publications

References 24 publications

Stem cell therapy for enhancement of bone consolidation in distraction osteogenesis

Stem cell therapy for enhancement of bone consolidation in distraction osteogenesis

Systemically administered MSCs given 24hrs after osteotomy do not affect bone formation in rat distraction osteogenesis

Adipose Derived Stem Cell on the Regeneration of Irradiated Mandible of Adult Rabbit Submitted to Distraction Osteogenesis

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