Effect of Pyrophosphate on the Ferredoxin: Nadp+ Oxidoreductase Activity in Vitro

Bojko, Monika; Wiçckowski, Stanislaw

doi:10.1007/978-94-011-3953-3_158

Cited by 1 publication

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“…It has been demonstrated that inhibition of this enzyme by only 20% can dramatically reduce the rate of ribulose-1,5-bisphosphate regeneration (Henkes et al, 2001). Second, there is evidence that diphosphate and its analogs are inhibitors of any Fd-dependent reaction (Forti and Meyer, 1969;Bojko and Wiȩckowski, 1999). Thus, the reduction in the rate of photosynthetic electron transport in bisphosphonate-inhibited leaves can result from the inhibition of Fd-NADP+ reductase (Fig.…”

Section: Why Is the Large Dark Pool Of Isoprenoid Substrates Not Convmentioning

confidence: 99%

“…However, there is evidence that diphosphate and its analogs are inhibitors of any ferredoxin (Fd)-dependent reaction (Forti and Meyer, 1969;Bojko and Wiȩckowski, 1999). This could be potentially relevant given that DXP/MEP pathwayreducing steps, at the level of 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate (HMBDP) synthase (HDS) and reductase (HDR), directly accept electrons from Fd in light (Eisenreich et al, 2001;Seemann et al, 2006;Li and Sharkey, 2013a).…”

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confidence: 99%

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Bisphosphonate Inhibitors Reveal a Large Elasticity of Plastidic Isoprenoid Synthesis Pathway in Isoprene-Emitting Hybrid Aspen

et al. 2015

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Recently, a feedback inhibition of the chloroplastic 1-deoxy-D-xylulose 5-phosphate (DXP)/2-C-methyl-D-erythritol 4-phosphate (MEP) pathway of isoprenoid synthesis by end products dimethylallyl diphosphate (DMADP) and isopentenyl diphosphate (IDP) was postulated, but the extent to which DMADP and IDP can build up is not known. We used bisphosphonate inhibitors, alendronate and zoledronate, that inhibit the consumption of DMADP and IDP by prenyltransferases to gain insight into the extent of end product accumulation and possible feedback inhibition in isoprene-emitting hybrid aspen (Populus tremula 3 Populus tremuloides). A kinetic method based on dark release of isoprene emission at the expense of substrate pools accumulated in light was used to estimate the in vivo pool sizes of DMADP and upstream metabolites. Feeding with fosmidomycin, an inhibitor of DXP reductoisomerase, alone or in combination with bisphosphonates was used to inhibit carbon input into DXP/MEP pathway or both input and output. We observed a major increase in pathway intermediates, 3-to 4-fold, upstream of DMADP in bisphosphonateinhibited leaves, but the DMADP pool was enhanced much less, 1.3-to 1.5-fold. In combined fosmidomycin/bisphosphonate treatment, pathway intermediates accumulated, reflecting cytosolic flux of intermediates that can be important under strong metabolic pull in physiological conditions. The data suggested that metabolites accumulated upstream of DMADP consist of phosphorylated intermediates and IDP. Slow conversion of the huge pools of intermediates to DMADP was limited by reductive energy supply. These data indicate that the DXP/MEP pathway is extremely elastic, and the presence of a significant pool of phosphorylated intermediates provides an important valve for fine tuning the pathway flux.

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Section: Why Is the Large Dark Pool Of Isoprenoid Substrates Not Convmentioning

confidence: 99%

mentioning

confidence: 99%

Bisphosphonate Inhibitors Reveal a Large Elasticity of Plastidic Isoprenoid Synthesis Pathway in Isoprene-Emitting Hybrid Aspen

et al. 2015

View full text Add to dashboard Cite

show abstract

Effect of Pyrophosphate on the Ferredoxin: Nadp+ Oxidoreductase Activity in Vitro

Cited by 1 publication

References 5 publications

Bisphosphonate Inhibitors Reveal a Large Elasticity of Plastidic Isoprenoid Synthesis Pathway in Isoprene-Emitting Hybrid Aspen

Bisphosphonate Inhibitors Reveal a Large Elasticity of Plastidic Isoprenoid Synthesis Pathway in Isoprene-Emitting Hybrid Aspen

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