“…However, isoprene synthase responsible for isoprene synthesis from DMADP has an exceptionally large K m value with respect to its substrate DMADP, two to three orders of magnitude greater than the K m values for mono-and sesquiterpene synthases to their substrates (Köksal et al, 2010). Thus, isoprene-emitting species typically support very high chloroplastic pools of DMADP and upstream metabolites that can sometimes reach impressive magnitudes (Li et al, 2011;Rasulov et al, 2011Rasulov et al, , 2015Li and Sharkey, 2013a). In fact, these pools can support isoprene emission in the darkness for extended periods of time (Li et al, 2011;Rasulov et al, 2011Rasulov et al, , 2015Li and Sharkey, 2013a).…”