Effect of pulsed millisecond current magnetic field on the proliferation of C6 rat glioma cells

Xu, Wenjun; Sun, Jinru; Le, Yangjing; Chen, Jingliang; Lu, Xiaoyun; Yao, Xueling

doi:10.1080/15368378.2019.1608233

Cited by 7 publications

(2 citation statements)

References 25 publications

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“…Recently, the use of ELF-MFs has been considered a safe alternative treatment for cancer (21). In this study, the effects of ELF-MFs on c-Myc and circ-CCDC66 expression changes in a gastric cell line (AGS) were evaluated.…”

Section: Discussionmentioning

confidence: 99%

Extremely Low Frequency Electromagnetic Fields Alter Expression of C-Myc and circ-CCDC66 in Gastric Cancer Cell Line

et al. 2022

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: The biological effects of electromagnetic fields (EMF), in particular their beneficial or adverse impacts on the promotion and progression of cancer, have attracted considerable attention. C-Myc has a critical regulatory role in cell transformation and causes metabolic changes, that accompany malignant transformation. Observations have shown that c-Myc expression can be altered by circular RNAs (circRNA). In this study, the changes in the expression of c-Myc and circ-CCDC66 in the AGS cell line after the extremely low-frequency magnetic fields (ELF-MF) exposure were investigated. The AGS cells were exposed to different magnetic flux densities for 18 h, continuously and discontinuously (2h on/2h off). To evaluate the expression changes of c-Myc and circ-CCDC66 real-time PCR was used. The results showed that discontinuous magnetic fields could reduce c-Myc expression. Continuous exposure of 0.25 mT could reduce the c-Myc expression, but with increasing the magnetic flux density c-Myc was upregulated. The expression level of circ-CCDC66 decreased under exposure to continuous and discontinuous ELF-MFs. The difference between the results of c-Myc under the influence of magnetic fields may be described by the hypothesis of the window effect of the fields. Our results indicated that ELF-MFs can induce changes in c-Myc and circ-CCDC66 expression.

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Section: Discussionmentioning

confidence: 99%

Extremely Low Frequency Electromagnetic Fields Alter Expression of C-Myc and circ-CCDC66 in Gastric Cancer Cell Line

et al. 2022

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“…Attention to electromagnetic exposure as a targeted tumor therapy is increasing recently (20). In addition, due to modern living conditions, exposure to electromagnetic fields caused by various devices is inevitable, which can be created from natural sources or by man-made sources such as diagnostic equipment, nuclear power plants, television receivers, and the like.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the PTEN and circRNA-CDR1as Gene Expression Changes in Gastric Cancer and Normal Cell Lines Following the Exposure to Weak and Moderate 50 Hz Electromagnetic Fields

et al. 2021

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Background: Attention to the electromagnetic exposure as a targeted tumor therapy has been recently increasing. Objectives: The aim of the current study was to investigate the effect of continuous and discontinuous electromagnetic fields on cell viability as well as phosphatase and tensin homolog (PTEN) and circular (circ)-RNA CDR1as genes expression in the normal and gastric cancer (GC) cell lines. Methods: After preparing gastric cancer cell lines (AGS) and normal cells (HU02 line), they were exposed to magnetic flux densities of 0.25, 0.5, 1, and 2 mT continuously and discontinuously (1h on/1h off) for 18 hours. The 3-(4,5-dimethylthiazoyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate cell viability. In addition, after designing the primers, the expression of the PTEN and circ-CDR1as genes was studied using the real-time polymerase chain reaction (real-time-PCR) technique. The results were analyzed using SPSS software version 25. Results: The exposed normal and tumor cells to discontinuous electromagnetic fields resulted in increasing of cell survival rate in both normal and tumor cells. In contrast, the exposure of continuous electromagnetic field showed no effect on the viability of the normal and tumor cells at intensities of 0.25, 0.5, and 1 mT. The electromagnetic field showed a significant effect on the expression of the circ-CDR1as gene and this effect depended on the intensity of the electromagnetic field used and the cell type. We have found that the activity of PTEN gene in the normal and tumor cells increased and decreased with increasing intensity of discontinuous electromagnetic field, respectively. Conclusions: In general, the effect of electromagnetic field on gastric cancer seems to depend on the kind of exposure as well as an extent of intensity and can be used for cancer therapeutic purposes. However, more research is needed on this subject.

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Low-frequency repetitive magnetic stimulation suppresses neuroblastoma progression by downregulating the Wnt/β-catenin signaling pathway

Seo

et al. 2022

Bioelectrochemistry

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Effect of pulsed millisecond current magnetic field on the proliferation of C6 rat glioma cells

Cited by 7 publications

References 25 publications

Extremely Low Frequency Electromagnetic Fields Alter Expression of C-Myc and circ-CCDC66 in Gastric Cancer Cell Line

Extremely Low Frequency Electromagnetic Fields Alter Expression of C-Myc and circ-CCDC66 in Gastric Cancer Cell Line

Evaluation of the PTEN and circRNA-CDR1as Gene Expression Changes in Gastric Cancer and Normal Cell Lines Following the Exposure to Weak and Moderate 50 Hz Electromagnetic Fields

Low-frequency repetitive magnetic stimulation suppresses neuroblastoma progression by downregulating the Wnt/β-catenin signaling pathway

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