Effect of pulsed light on postharvest disease control-related metabolomic variation in melon (Cucumis melo) artificially inoculated with Fusarium pallidoroseum

Abstract

<jats:title>Abstract</jats:title><jats:p>Pulsed light, as a postharvest technology, is an alternative to traditional fungicides, and can be used on a wide variety of fruit and vegetables for sanitization or pathogen control. In addition to these applications, other effects also are detected in vegetal cells, including changes in metabolism and production of secondary metabolites, which directly affect disease control response mechanisms. This study aimed to evaluate the possible applications of pulsed ultraviolet light in controlling postharvest rot, mainly caused by <jats:italic>Fusarium pallidoroseum</jats:italic> in yellow melon ‘Goldex’, <jats:italic>in natura</jats:italic>, and its implications in the disease control as a function of metabolomic expression to effect fungicidal or fungistatic. The dose of pulsed light (PL) that inhibited <jats:italic>F. pallidoroseum</jats:italic> growth in melons (<jats:italic>Cucumis</jats:italic> melo var. Spanish) was 9 KJ m<jats:sup>-2</jats:sup>. Ultra performance liquid chromatography (UPLC) coupled to a quadrupole time-of-flight (QTOF) mass analyzer identified 12 compounds based on the MS/MS fragmentation patterns. Chemometric analysis by Principal Components Analysis (PCA) and Orthogonal Partial Least Squared Discriminant Analysis (OPLS-DA and S-plot) were used to evaluate the changes in fruit metabolism. PL technology provided protection against postharvest disease in melons, directly inhibiting the growth of <jats:italic>F. pallidoroseum</jats:italic> through upregulation of specific fruit biomarkers such as pipecolic acid (<jats:bold>11</jats:bold>), saponarin (<jats:bold>7</jats:bold>), and orientin (<jats:bold>3</jats:bold>), which acted as major markers for the defense system against pathogens. PL can thus be proposed as a postharvest technology to avoid chemical fungicides and may be applied to reduce the decay of melon quality during its export and storage.</jats:p>

Controles alternativos no manejo da podridão por Fusarium sp. em pós-colheita do melão: uma revisão

“…Em outro estudo, Filho et al, (2020) observaram que o tratamento com Uvp forneceu proteção contra doenças póscolheita em melões, inibindo diretamente o crescimento de Fusarium pallidoroseum através da regulação positiva de biomarcadores específicos de frutas, como o ácido pipecólico, saponarina e orientina, que atuou como um dos principais marcadores do sistema de defesa contra patógenos.…”

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“…Research on the influence of UV irradiation (single exposure) on the most frequent postharvest fungal infections and human pathogens, such as Monilinia spp, Rhizopus, Penicillum species, Fusarium spp, Botrytis cinerea and Colletrichcum spp. Have been conducted (Filho et al, 2020).…”

Preharvest high-intensity, pulsed polychromatic light and low-intensity UV-C treatments control Botrytis cinerea on lettuce (Lactuca sativa)

“…A second study looking into HIPPL disease control showed that a 9 kJ/m 2 treatment reduced the mean lesion area of Fusarium pallidoroseum from 13 mm to 4 mm on melon (Cucumis melo) at 21 d post inoculation (DPI) (Filho et al 2020). Incidence was reduced from 100% to 33% when compared to the control.…”