Effect of pulsed electric fields and high pressure homogenization on the aqueous extraction of intracellular compounds from the microalgae Chlorella vulgaris

Carullo, Daniele; Abera, Biresaw Demelash; Casazza, Alessandro Alberto; Donsı̀, Francesco; Perego, Patrizia; Ferrari, Giovanna; Pataro, Gianpiero

doi:10.1016/j.algal.2018.01.017

Cited by 161 publications

(134 citation statements)

References 48 publications

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“…where C-CP is C-phycocyanin content in mg/g, P: is pressure in the bar, and S: is the solvent type (Table 4). Our results showed that after each disruption, the amounts of recovered C-PC from A. maxima increased in direct correlation with a higher operating pressure, which is confirmed by what is described by Halim, et al [34]. However, we also observed that the increase from 1400 to 1600 bar caused a decrease in the C-PC content of A. maxima.…”

Section: Optimization Of Hph Extraction Conditions For C-pc Of Arthrosupporting

confidence: 92%

Rapid Green Extractions of C-Phycocyanin from Arthrospira maxima for Functional Applications

et al. 2019

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Cyanobacteria are a rich source of bioactive compounds, mainly in the Arthospira sp., and one of the most interesting components in recent years has been C-phycocyanin (C-PC). There have been several conventional methods for their extraction, among which stand out: chemical products, freezing-thawing (FT); enzymatic, and maceration (M); which have come to be replaced by more environmentally friendly methods, such as those assisted by microwaves (MW) and high-pressure homogenization (HPH). The aim of the research was to use these two “green extraction processes” to obtain C-PC from cyanobacteria Arthrospira maxima because they improve functionality and are fast. Extractions of C-PC were studied by means of two experimental designs for MW and HPH, based on a response surface methodology (RSM) employing, firstly, a factorial design 33: power (100, 200, and 300 W), time (15, 30, and 60 s), and types of solvents (distiller water, Na-phosphate buffer and, distiller water: Na-phosphate buffer (Ph 7.0; 1:1, v/v); and secondly, two factors with different levels: Pressure (800, 1000, 1200, 1400, and 1600 bar) and, types of solvents (distilled water, Na-phosphate buffer (pH 7.0) 100 mM and, Na-phosphate buffer:water 1:1, (v/v)). Optimum C-PC content was achieved with the HPH process under Na-phosphate solvent at 1400 bar (291.9 ± 6.7 mg/g) and the MW method showed improved results using distilled water as a solvent at 100 W for 30 s (215.0 ± 5.5 mg/g). In the case of conventional methods, the freeze–thawing procedure reached better results than maceration using the buffer (225.6 ± 2.6 mg/g). This last one also did not show a significant difference between solvents (a range of 147.7–162.0 mg/g). Finally, the main advantage of using green extractions are the high C-PC yield achieved, effectively reducing both processing times, costs, and increasing the economic and functional applications of the bioactive compound.

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Section: Optimization Of Hph Extraction Conditions For C-pc Of Arthrosupporting

confidence: 92%

Rapid Green Extractions of C-Phycocyanin from Arthrospira maxima for Functional Applications

et al. 2019

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“…Additionally, it should be investigated whether a natural autolysis of microalgae cells induced for example by starvation could induce the same effects as PEFtreatment especially on lipid extraction. Note that in a recent published study on the effect of PEF-treatment on Chlorella vulgaris, very different results were reported since time course measurements of conductivity after PEF-treatment showed stabilization after about 1h(Carullo et al, 2018). The longer pulses used in this study, which are less prone to induce intracellular damages might partially explain the observed differences.…”

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confidence: 77%

Incubation time after pulsed electric field treatment of microalgae enhances the efficiency of extraction processes and enables the reduction of specific treatment energy

Silve

Kian

Papachristou

et al. 2018

Bioresource Technology

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Pulsed Electric Field (PEF) pre-treatment, applied on fresh microalgae Auxenochlorella protothecoides, induces spontaneous release of a substantial water fraction and enables subsequent lipid extraction using ethanol-hexane blends. In this study, fresh microalgae suspensions were treated with PEF and incubated under inert conditions. Incubation promotes the release of ions and carbohydrates and increases the yields of subsequent lipid extraction thus enabling a considerable reduction of PEF-treatment energy. With a 20 h incubation period at 25 °C, almost total lipid extraction is achieved with a specific PEF-treatment energy of only 0.25 MJ/kg. Incubation on ice remains beneficial but less efficient than at 25 °C. Additionally, incubating microalgae cells in suspension at 100g/L or in a dense paste, was almost equally efficient. Correlation between the different results suggests that spontaneous release of ions and carbohydrates facilitates more successful lipid extraction. A direct causality between the two phenomena remains to be demonstrated.

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“…The treatment improves extraction selectively, and thus the extracts obtained by PEF-assisted method are purer than those obtained using other methodologies that consist of the total destruction of cells such as bead-beating, crushing, ultrasound, or high pressure homogenization producing release of impurities. The energy consumption of PEF is much lower than in those conventional techniques (Carullo et al, 2018;de Boer, Moheimani, Borowitzka, & Bahri, 2012). In addition, regarding nonpolar compounds recovery from microalgae, which constitute a promising source of these metabolites, PEF treatment followed by autolysis induction during aqueous incubation permits to substitute the injurious organic chemicals by more safe and ecofriendly solvents (Martínez, Gojkovic et al, 2019c).…”

Section: Pef-assisted Extraction From Microalgaementioning

confidence: 99%

Pulsed electric field‐assisted extraction of valuable compounds from microorganisms

Martínez

Delso

Álvarez

et al. 2020

Comp Rev Food Sci Food Safe

127

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Microorganisms (bacteria, yeast, and microalgae) are a promising resource for products of high value such as nutrients, pigments, and enzymes. The majority of these compounds of interest remain inside the cell, thus making it necessary to extract and purify them before use. This review presents the challenges and opportunities in the production of these compounds, the microbial structure and the location of target compounds in the cells, the different procedures proposed for improving extraction of these compounds, and pulsed electric field (PEF)‐assisted extraction as alternative to these procedures. PEF is a nonthermal technology that produces a precise action on the cytoplasmic membrane improving the selective release of intracellular compounds while avoiding undesirable consequences of heating on the characteristics and purity of the extracts. PEF pretreatment with low energetic requirements allows for high extraction yields. However, PEF parameters should be tailored to each microbial cell, according to their structure, size, and other factors affecting efficiency. Furthermore, the recent discovery of the triggering effect of enzymatic activity during cell incubation after electroporation opens up the possibility of new implementations of PEF for the recovery of compounds that are bounded or assembled in structures. Similarly, PEF parameters and suspension storage conditions need to be optimized to reach the desired effect. PEF can be applied in continuous flow and is adaptable to industrial equipment, making it feasible for scale‐up to large processing capacities.

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Effect of pulsed electric fields and high pressure homogenization on the aqueous extraction of intracellular compounds from the microalgae Chlorella vulgaris

Cited by 161 publications

References 48 publications

Rapid Green Extractions of C-Phycocyanin from Arthrospira maxima for Functional Applications

Rapid Green Extractions of C-Phycocyanin from Arthrospira maxima for Functional Applications

Incubation time after pulsed electric field treatment of microalgae enhances the efficiency of extraction processes and enables the reduction of specific treatment energy

Pulsed electric field‐assisted extraction of valuable compounds from microorganisms

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