Effect of pulsed electric field on diabetes-induced glycated enzyme, oxidative stress, and inflammatory markers in vitro and in vivo

Abstract

This study focused on the effect of pulsed electric field (PEF) on the modification of superoxide dismutase (SOD) to recover its activity (in vitro) and thereby reverse oxidative stress in diabetic mice (in vivo). The result of the in vitro assay demonstrated that PEF could modify the glycated SOD structure through a change in the electrostatic environment of the protein. This modification leads to an 83% and 31% increase in the enzymatic activity of in vitro and in vivo assays, respectively. The in vivo assessment results show a decrease in oxidative stress biomarker indices, advanced glycation end products content, and inflammatory cytokines concentrations. However, the change in blood glucose level is not significant. According to the results, PEF would be effective in diabetes therapy and could be employed as a complementary method.

Effects of pulsed electric field technology on the nutritional value and biological function of plant food

“…Furthermore, the cytoprotective capacity of PEF extracts was linearly related to total carotenoid content and total anthocyanin content. For Yellow Solar carrots, which had a low total carotenoid content, PEF significantly increased the carotenoid content (Leong et al, 2016a;Rezaeinezhad et al, 2022). Leong et al (2016b) also found that PEF treatment shortened the immersion time and improved the protection against H 2 O 2 oxidative stress in Vitis Vinifera.…”

“…found that the antioxidant capacity of PEF-treated microalgae bio extracts was significantly enhanced. PEF can increase the number of nutrients synthesized in plant-based foods, which can be utilized to customize extraction parameters for low levels of active substances and synthesize more of the nutrients required by humans(Leong et al, 2016a;Rezaeinezhad et al, 2022).…”