Effect of puerarin on bone formation

Wong, Ricky W.K.; Rabie, Bakr

doi:10.1016/j.joca.2007.02.009

Cited by 70 publications

(63 citation statements)

References 34 publications

(42 reference statements)

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“…The active ingredients have been identified as the isoflavones: puerarin, daidzin and daidzein, which have estrogenic activity (21)(22)(23)(24). Puerariae radix has shown positive therapeutic effects in animal studies (25)(26)(27) and in cell culture studies, Puerariae radix was shown to induce osteogenic activity (20,(28)(29)(30)(31). The studies showed that Puerariae radix had no effect on viability, but enhanced Alkaline phosphatase (ALP activity), vascular endothelial growth factor (VEGF), bone matrix proteins such as osteocalcin (OCN), osteopontin (OPN), and collagen type I, and mineralization (20).…”

Section: Introductionmentioning

confidence: 99%

Effects of a surface topography composite with puerariae radix on human STRO-1-positive stem cells

et al. 2010

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Section: Introductionmentioning

confidence: 99%

Effects of a surface topography composite with puerariae radix on human STRO-1-positive stem cells

et al. 2010

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“…Genistein were used in both prophylaxis and treatment of diseases in recent years, acting such as antioxidant (Ganai & Farooqi 2015;Kanazawa et al 1995), anticancer properties (Sarkar & Li 2002;Qi et al 2011), cardioprotection (Deodato et al 1999) and immunosupressor agent (Yellayi et al 2002). Puerarin has been demonstrated to possess a variety of beneficial activities on alleviating angiocardiopathy (Pan et al 2009), osteoporosis (Wong & Rabie 2007) and hangover (Penetar et al 2012). In the process of daidzein biosynthesis, the important step is that FIGURE 5.…”

Section: Discussionmentioning

confidence: 99%

Expression and Functional Analysis of a Transgenic Cytochrome P450 Monooxygenase in Pueraria mirifica

Xu¹,

Kaopong²,

Nualkaew³

et al. 2017

JSM

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“…This result was consistent with the report of Tiyasatkulkovit et al 9) Studies exhibited that puerarin in collagen matrix increased new bone formation locally and could be used for bone graft and bone induction. 15) Further, there were data suggested that puerarin stimulated osteoblastic proliferation and Akt activation via phosphatidylinositol 3-kinase (PI3K)-dependent manner, 16) or through caspase-3, 17) estrogen receptor, p38 mitogen-activated protein kinase (MAPK), and Wnt/β-catenin pathways.…”

Section: Fig 2 Microtomographic Histomorphometry Of the Proximal Tibiamentioning

confidence: 99%

Puerarin Enhances Bone Mass by Promoting Osteoblastogenesis and Slightly Lowering Bone Marrow Adiposity in Ovariectomized Rats

Liu

Jia

2014

Biological & Pharmaceutical Bulletin

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We investigated the effect of puerarin on bone mass and marrow adiposity in ovariectomy (OVX)-induced osteoporosis. The rats were divided into four groups: control; OVX; OVX estradiol (OVX-E); and OVX puerarin treatment (OVX-GE). In vivo, bone mineral density (BMD) and histomorphometry were measured under microCT. The mechanical properties of tibia were obtained in 3-point bending test. Plasma osteocalcin and adiponectin were determined using enzyme-linked immunosorbent assay (ELISA). Alkaline phosphatase (ALP) were measured using biochemical methods. In vitro, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Oil Red O staining were used to compare osteoblast proliferation and adipocyte differentiation, respectively. Osteocalcin and adiponectin in culture supernatants were determined using ELISA. The results showed that puerarin significantly enhanced bone volume density and trabecular number compared with OVX and OVX-E groups (p<0.05, p<0.05, respectively). Puerarin increased energy to ultimate load, plasma osteocalcin and ALP (p<0.01). However, BMD in OVX-GE group was less than that in control (p<0.01) and OVX-E groups (p<0.05). The culture supernatants from OVX-GE group showed increased osteocalcin compared with those from OVX ( p<0.01) and OVX-E groups ( p<0.05). Puerarin lowered adiponectin in culture supernatant compared with supernatant from OVX group and inhibited the increase in adipocytes caused by OVX (p<0.01). However, the amount of lipids did not differ between OVX-GE and OVX groups. These findings suggest that puerarin likely enhances bone formation by stimulating the proliferation and differentiation of osteoblasts while slightly inhibiting the adipotic differentiation.Key words puerarin; bone mass; bone formation; adiposity; osteoporosis Puerariae radix, the root of Pueraria lobata (WILD.) OTRWI, a wild creeper leguminous plant, is one of the earliest and most important crude herbs used in Chinese medicine for various medicinal purposes. For the near decade, it has been reported using osteoporosis animal model that total isoflavones of puerariae isoflavone could protect osteoporosis induced by ovariectomized rats 1,2) and could inhibit the secondary osteoporosis induced by dexamethasone in rats.3) As a kind of phytoestrogen, puerarin is regarded as one of isoflavone extract which exerting preventive effect on bone loss in ovariectomized rats.4) However, all the related animal experiment used total isoflavones of Pueraria lobata (WILD.) OTRWI. Their results were the comprehensive effects of daidzein, genistein and puerarin. We couldn't judge the independent effect of puerarin on bone gain.Puerarin is an active component extracted from the Chinese traditional medicine Pueraria lobata (WILD.) OTRWI. Structurely, puerarin is similar to estrogen. It has three -OH, where they combine with estradiol receptors and then exhibit estrogenic action. Therefore, it's believed to exhibit beneficial effect on bone tissue by mimicking the biological responses of estrogens.5,6) Previously, we...

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Effect of puerarin on bone formation

Abstract: Puerarin in collagen matrix has the effect of increasing new bone formation locally and can be used for bone grafting or for bone induction often required in surgery.

Cited by 70 publications

References 34 publications

Effects of a surface topography composite with puerariae radix on human STRO-1-positive stem cells

Effects of a surface topography composite with puerariae radix on human STRO-1-positive stem cells

Expression and Functional Analysis of a Transgenic Cytochrome P450 Monooxygenase in Pueraria mirifica

Puerarin Enhances Bone Mass by Promoting Osteoblastogenesis and Slightly Lowering Bone Marrow Adiposity in Ovariectomized Rats

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