Effect of protective agents, rehydration media and initial cell concentration on viability of Pantoea agglomerans strain CPA-2 subjected to freeze-drying

Costa, E.; Usall, J.; Teixidó, Neus; Garcia, Nayara Fernanda Lisbôa; Viñas, I.

doi:10.1046/j.1365-2672.2000.01182.x

Cited by 158 publications

(105 citation statements)

References 34 publications

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“…The major damagesr esulting fromf reezing are fusion and thermotropic phase transitions of the cellmembranes.Cryoprotectants can preventthisvia the increase in the charged ensity around the headgroups of the biolayer and therefore inhibiting fusion by electrostatic repulsion, or they can limit fusion by steric hinderance. Beside the used protectivea gents the initialc elll oad, the rehydration media [18], as well as the growing conditions [ 19] influence the resulting viability after freeze-drying. Another important parameter affecting the stability of the producti st he value of the water activity (a w -value).…”

Section: Introductionmentioning

confidence: 99%

Effect of protective agents on the viability of Lactococcus lactis subjected to freeze-thawing and freeze-drying

Berner

2006

Sci Pharm

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The effecto fd ifferentp rotectants and the impact of the initial cell densityo n the viability of Lactococcus lactis Sr. 3.54 subjected to freeze-thawing and freezedrying was studied. Several additivesw ere tested as protective agents against freezing and drying injuries. Maximumv iability of the cells after freeze-thawing was obtained with sucrose and skim milk mixtures as protective agents (78% viability).

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Section: Introductionmentioning

confidence: 99%

Effect of protective agents on the viability of Lactococcus lactis subjected to freeze-thawing and freeze-drying

Berner

2006

Sci Pharm

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“…Protective additives have an important role in the conservation of viability. A good protectant should provide cryoprotection of cells during freezedrying, be easily dried, and provide a good matrix to allow stability and ease of rehydration [4]. Sorbitol and monosodium glutamate (MSG) have been reported as efficient protectants [1,11,17,22].…”

Section: Introductionmentioning

confidence: 99%

Protective effect of sorbitol and monosodium glutamate during storage of freeze-dried lactic acid bacteria

Carvalho

Silva

et al. 2003

Lait

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-The effects of sorbitol and monosodium glutamate upon survival during storage of freeze-dried Lactobacillus bulgaricus, Lactobacillus plantarum, Lactobacillus rhamnosus, Enterococcus durans and Enterococcus faecalis were examined. There were no significant differences in survival during freeze-drying after addition of sorbitol or monosodium glutamate. However, these compounds were found to increase the stability of most strains during long-term storage. Various survival patterns were observed among the strains considered. However, for most of them, both compounds were proven effective in maintaining high degrees of viability over long periods of storage.Starter culture / preservation / additive / processing / viability Résumé -Effet protecteur du sorbitol et du glutamate monosodique pendant le stockage de bactéries lactiques lyophilisées. Les effets du sorbitol et du glutamate monosodique sur la survie des souches lyophilisées de Lactobacillus bulgaricus, Lactobacillus plantarum, Lactobacillus rhamnosus, Enterococcus durans et Enterococcus faecalis pendant le stockage ont été étudiés. On n'a pas trouvé de différences significatives pendant la lyophilisation, après l'addition de sorbitol ou de glutamate monosodique. Cependant, l'addition de ces composés a augmenté la stabilité de la plupart des souches pendant le stockage prolongé. Différents profils de survie ont été observés parmi les souches considérées ; toutefois, pour la plupart des souches les deux composés étaient efficaces dans le maintien de degrés élevés de viabilité pendant de longues périodes de stockage. Levain / conservation / additif / traitement / viabilité

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“…Teixidó et al, 2001;Nunes et al, 2001Nunes et al, , 2002aTorres et al, 2007). Other work related to the modes of action of this strain and to its production and viability has also been published (Costa et al, 2000(Costa et al, , 2001(Costa et al, , 2002aPoppe et al, 2003). This indicates that P. agglomerans strain CPA-2 could serve as the basis of a new biocontrol product.…”

Section: Discussionmentioning

confidence: 91%

Development and application of a SCAR marker to monitor and quantify populations of the postharvest biocontrol agent Pantoea agglomerans CPA-2

Nunes

Bajji

Stepien

et al. 2008

Postharvest Biology and Technology

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Pantoea agglomerans CPA-2 is an effective biocontrol agent of postharvest diseases of citrus and pome fruit. A monitoring technique was developed for its identification and to quantify its populations. The methodology used consisted of (i) searching for a semi-selective medium, (ii) identification of molecular markers and (iii) monitoring population dynamics in a commercial trial. As a semi-selective medium, Malonate Broth Agar supplemented with tetracycline hydroxychloride and incubation at high temperature (max. of 40 • C) facilitated the selective recovery of P. agglomerans CPA-2 colonies. The RAPD technique was applied to a collection of 13 strains of P. agglomerans, including CPA-2. Among the 12 primers tested, OPL-11 amplified a fragment (about 720 bp) specific to strain CPA-2. On the basis of this fragment, two SCAR markers were amplified using a primer pair derived from OPL-11 elongation. A first SCAR marker of 720 bp was specifically amplified for the strain CPA-2 and a second one of 270 bp was obtained for all P. agglomerans strains tested, including CPA-2. Commercial trials demonstrated a significant reduction of decay with the treatment of formulated cells of P. agglomerans CPA-2. Population dynamics of CPA-2 in commercial trials were determined on fruit surfaces and in the environment using both the classical plating technique and PCR with SCAR primers. In general, no significant differences were observed between results obtained from the two methods. On fruit surfaces, 1 day after CPA-2 applied its population by classical methods was 4.37 × 10 6 cfu wound −1 and at the end of the experiment the population increased to 5.8 × 10 5 cfu wound −1 . The percentages of colonies identified as P. agglomerans CPA-2 at these sampling times using SCAR primers were 90 and 95%, respectively. Population dynamics in the environment to evaluate the environmental fate of P. agglomerans CPA-2 showed that it has a limited persistence and limited capacity for dispersion.

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Effect of protective agents, rehydration media and initial cell concentration on viability of Pantoea agglomerans strain CPA-2 subjected to freeze-drying

Cited by 158 publications

References 34 publications

Effect of protective agents on the viability of Lactococcus lactis subjected to freeze-thawing and freeze-drying

Effect of protective agents on the viability of Lactococcus lactis subjected to freeze-thawing and freeze-drying

Protective effect of sorbitol and monosodium glutamate during storage of freeze-dried lactic acid bacteria

Development and application of a SCAR marker to monitor and quantify populations of the postharvest biocontrol agent Pantoea agglomerans CPA-2

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