Effect of propolis, some isolated compounds and its source plant on antibody production

Propolis and its constituent caffeic acid suppress LPS-stimulated pro-inflammatory response by blocking NF-κB and MAPK activation in macrophages, Journal of Ethnopharmacology, http://dx.doi.org/ 10. 1016/j.jep.2013.06.004 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractEthnopharmacological relevance: Propolis is a bee product with numerous biological and pharmacological properties, such as immunomodulatory and anti-inflammatory activities.It has been used in folk medicine as a healthy drink and in food to improve health and prevent inflammatory diseases. However, little is known about its mechanism of action.Thus, the goal of this study was to verify the antioxidant activity and to explore the antiinflammatory properties of propolis by addressing its intracellular mechanism of action.Caffeic acid was investigated as a possible compound responsible for propolis action. Materials and Methods:The antioxidant properties of propolis and caffeic acid were evaluated by using the 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) scavenging method. To analyze the anti-inflammatory activity, Raw 264.7 macrophages were treated with different concentrations of propolis or caffeic acid, and nitric oxide (NO) production, a strong pro-inflammatory mediator, was evaluated by the Griess reaction. The concentrations of propolis and caffeic acid that inhibited NO production were evaluated on intracellular signaling pathways triggered during inflammation, namely p38 mitogenactivated protein kinase (MAPK), c-jun NH 2 -terminal kinase (JNK1/2), the transcription nuclear factor (NF)-NB and extracellular signal-regulated kinase (ERK1/2), through Western blot using specific antibodies. A possible effect of propolis on the cytotoxicity of hepatocytes was also evaluated, since this product can be used in human diets.Results: Caffeic acid showed a higher antioxidant activity than propolis extract. Propolis and caffeic acid inhibited NO production in macrophages, at concentrations without cytotoxicity. Furthermore, both propolis and caffeic acid suppressed LPS-induced signaling pathways, namely p38 MAPK, JNK1/2 and NF-NB. ERK1/2 was not affected by propolis extract and caffeic acid. In addition, propolis and caffeic acid did not induce hepatotoxicity at concentrations with strong anti-inflammatory potential. Conclusions:Propolis exerted an antioxidant and anti-inflammatory action and caffeic acid may be involved in its inhibitory effects on NO production and intracellular signaling cascades, suggesting its use as a natural source of safe anti-inflammatory drugs.

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“…After a week, extracts were filtered and the dry weight of the extracts was calculated (110 mg/mL) (Sforcin et al, 2005).…”

Section: Propolis Sample and Caffeic Acidmentioning

confidence: 99%

Propolis and its constituent caffeic acid suppress LPS-stimulated pro-inflammatory response by blocking NF-κB and MAPK activation in macrophages

Búfalo

Ferreira

Costa

et al. 2013

Journal of Ethnopharmacology

155

102

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“…Propolis (bee resin) has also been studied and stimulates antibody production. 24 A major problem with the investigation and use of herbal products is the lack of standardisation. 25 A recent meta-analysis by Shah et al found that Echinacea decreased the odds of developing the common cold by 58% and the duration of a cold by 1.4 days.…”

Section: Complementary and Alternative Medicine (Cam) For Urtimentioning

confidence: 99%

Management of upper respiratory tract infections in children

Cotton

Innes

Jaspan

et al. 2008

South African Family Practice

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Upper respiratory tract infection (URTI) occurs commonly in both children and adults and is a major cause of mild morbidity. It has a high cost to society, being responsible for absenteeism from school and work and unnecessary medical care, and is occasionally associated with serious sequelae. URTIs are usually caused by several families of virus; these are the rhinovirus, coronavirus, parainfluenza, respiratory syncytial virus (RSV), adenovirus, human metapneumovirus, influenza, enterovirus and the recently discovered bocavirus. This review will mainly focus on the rhinovirus, where significant advances have been made in understanding the epidemiology, natural history and relationship with other pathogens.

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“…8,9 A proporção dos compostos fenólicos é variável e também depende do local e da época da coleta. 1,3,10 Devido à composição química complexa e variável da própolis, várias são as atividades biológicas relatadas na literatura, tais como antimicrobiana, 5,11,12,13 anticariogêni-ca, 3,14,15 citotóxica, 5,8,12,16,17 anti-inflamatória, 18 imunomodulatória, 12,19,20 antioxidante 5,11,21 e antitumoral. 22 A amplitude das atividades biológicas da própolis é maior em áreas tropicais do planeta, refletindo a diversidade vegetal destas regiões.…”

Section: Introductionunclassified

Composição fenólica, atividade antibacteriana e antioxidante da própolis vermelha brasileira

Cabral¹,

Oldoni²,

Prado³

et al. 2009

Quím. Nova

132

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Recebido em 12/8/08; aceito em 4/2/09; publicado na web em 3/7/09 PHENOLIC COMPOSITION, ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF BRAZILIAN RED PROPOLIS. Propolis is a resinous hive product collected by honeybees from various plant sources. It has a complex chemical composition, constituted by various phenolic compounds. Extracts of increasing polarity (n-hexane, chloroform, and ethanol) were obtained from a sample of red propolis from the state of Alagoas. Assays were carried out for determination of contents of phenolics, along with antibacterial and antioxidant activities. The EEP, fractions and sub-fractions showed strong biological activities and were related with phenolic the content compounds contents. The sub-fractions were more bioactive than the EEP and fractions, demonstrating that the antioxidant and antibacterial activities are not a result of synergistic effect between the various chemical compounds in propolis.

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Effect of propolis, some isolated compounds and its source plant on antibody production

Cited by 122 publications

References 25 publications

Propolis and its constituent caffeic acid suppress LPS-stimulated pro-inflammatory response by blocking NF-κB and MAPK activation in macrophages

Propolis and its constituent caffeic acid suppress LPS-stimulated pro-inflammatory response by blocking NF-κB and MAPK activation in macrophages

Management of upper respiratory tract infections in children

Composição fenólica, atividade antibacteriana e antioxidante da própolis vermelha brasileira

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